DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ glatt-2015-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264715X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264724X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264718X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264720X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-5 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264721X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264722X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264717X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-8 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264719X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-9 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264716X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation glatt-2015-10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264723X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions imex curation ats1_yeast Protein KTI13 Alpha-tubulin suppressor 1 ATS1 KTI13 Kluyveromyces lactis toxin-insensitive protein 13 FUN28 YAL006 YAL020C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSCVYAFGSNGQRQLGLGHDEDMDTPQRSVPGDDGAIVRKIACGGNHSVMLTNDGNLVGCGDNRRGELDSAQALRQVHDWRPVEVPAPVVDVACGWDTTVIVDADGRVWQRGGGCYEFTQQHVPLNSNDERIAVYGCFQNFVVVQGTRVYGWGSNTKCQLQEPKSRSLKEPVLVYDTGSVAVDYVAMGKDFMVIVDEGGRIVHASGRLPTGFELKQQQKRHNLVVLCMWTSIHLWNARLNTVESFGRGTHSQLFPQERLDFPIVGVATGSEHGILTTANQEGKSHCYNVYCWGWGEHGNCGPQKGSQPGLQLVGQYSGKPRVFGGCATTWIVL 3C6F4BE180479096 dph3_yeast Diphthamide biosynthesis protein 3 DPH3 Kluyveromyces lactis toxin-insensitive protein 11 YBL071W-A KTI11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTYDEIEIEDMTFEPENQMFTYPCPCGDRFQIYLDDMFEGEKVAVCPSCSLMIDVVFDKEDLAEYYEEAGIHPPEPIAAAA 8B27A307831B0ECE dph1_yeast 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 DPH1 Diphthamide biosynthesis protein 1 Diphtheria toxin resistance protein 1 S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1 YIL103W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGSTESKKQPRRRFIGRKSGNSNNDKLTTVAENGNEIIHKQKSRIALGRSVNHVPEDILNDKELNEAIKLLPSNYNFEIHKTVWNIRKYNAKRIALQMPEGLLIYSLIISDILEQFCGVETLVMGDVSYGACCIDDFTARALDCDFIVHYAHSCLVPIDVTKIKVLYVFVTINIQEDHIIKTLQKNFPKGSRIATFGTIQFNPAVHSVRDKLLNDEEHMLYIIPPQIKPLSRGEVLGCTSERLDKEQYDAMVFIGDGRFHLESAMIHNPEIPAFKYDPYNRKFTREGYDQKQLVEVRAEAIEVARKGKVFGLILGALGRQGNLNTVKNLEKNLIAAGKTVVKIILSEVFPQKLAMFDQIDVFVQVACPRLSIDWGYAFNKPLLTPYEASVLLKKDVMFSEKYYPMDYYEAKGYGRGETPKHAIE 22E93852E03C6722 elp3_yeast Elongator complex protein 3 ELP3 Gamma-toxin target 3 HPA1 TOT3 tRNA uridine(34) acetyltransferase YPL086C protein protein yeast Saccharomyces cerevisiae Baker's yeast MARHGKGPKTNKKKLAPEKERFIQCCADITLELTDSLTSGTTREINLNGLITKYSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRVEQLKQLGHSIDKVEYVLMGGTFMSLPKEYREDFIVKLHNALSGFNGNDIDEAILYSQQSLTKCVGITIETRPDYCTQTHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVRSVCETFAVSKDAGYKVVSHMMPDLPNVGMERDIEQFKEYFENPDFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYSANALVDLVARILALVPPWTRIYRVQRDIPMPLVTSGVDNGNLRELALARMKDLGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKKDILIGLLRLRKASKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAKEEHGSEKISVISGVGVRNYYGKLGYELDGPYMSKRI 64EF8B42D3C3102E kti11-kti13-1 3273696 N/A 3273706 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3273707 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q3E840:p.Tyr4Ala Y4A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Asp5Ala D5A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Glu8Ala E8A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 1-7 Region 1-7 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false kti11-dph1-4 3273697 N/A 3273708 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3273707 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q3E840:p.Cys50Ser C50S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Cys25Ser C25S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Cys27Ser C27S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Cys47Ser C47S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false kti11-kti13-2 3273698 N/A 3273706 unspecified role unspecified role unspecified role unspecified role P31386:p.Trp229Ala W229A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P31386:p.Glu296Ala E296A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P31386:p.His297Ala H297A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3273707 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false kti11-kti13-3 3273699 N/A 3273706 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3273707 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false kti11-kti13-4 3273700 N/A 3273707 unspecified role unspecified role bait bait Q3E840:p.Tyr4Ala Y4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 1-7 Region 1-7 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Tyr4_Asp5delinsAlaAla Y4A/D4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q3E840:p.[Ile7Ala;Ile9Ala] I7A/I9A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3273706 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false kti11-kti13-5 3273701 N/A 3273707 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3273706 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P31386:p.Trp229Cys W229C mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false kti11-kti13-6 3273702 N/A 3273706 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3273707 unspecified role unspecified role unspecified role unspecified role Q3E840:p.Tyr4Ala Y4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Asp5Ala D5A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Tyr4_Asp5delinsAlaAla Y4A/D4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3E840:p.Glu8Ala E8A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3E840:p.[Glu6Ala;Glu8Ala] E6A/E8A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false kti11-kti13-7 3273703 N/A 3273706 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3273707 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false kti11-kti13-8 3273704 N/A 3273706 unspecified role unspecified role prey prey P31386:p.Trp229Cys W229C mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P31386:p.Trp229Ala W229A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P31386:p.Trp294Ala W294A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3273707 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false kti11-elp3-1 3273705 N/A 3273709 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3273707 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q3E840:p.Cys50Ser C50S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false