IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ muia-2014-4 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. esadler@dom.wustl.edu Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Only protein-protein interactions muia-2014-1 in vitro In vitro protease assay protease assay tag fluorescence tag visualisation by fluorescence tag fluorescence Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. Cleavage was detected as an increase in fluorescence esadler@dom.wustl.edu Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Only protein-protein interactions muia-2014-2 in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. Plasma VWF was vortexed (with ADAMTS13 variants) to imitate fluid shear stress and start cleavage of multimeric VWF. esadler@dom.wustl.edu Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Only protein-protein interactions muia-2014-3 in vitro In vitro protease assay protease assay weight by staining molecular weight estimation by staining weight by staining Muia J., Zhu J., Gupta G., Haberichter SL., Friedman KD., Feys HB., Deforche L., Vanhoorelbeke K., Westfield LA., Roth R., Tolia NH., Heuser JE., Sadler JE. esadler@dom.wustl.edu Proceedings of the National Academy of Sciences of the United States of America 2014 imex curation Only protein-protein interactions vwf_human von Willebrand factor VWF F8VWF protein protein human Homo sapiens Human MIPARFAGVLLALALILPGTLCAEGTRGRSSTARCSLFGSDFVNTFDGSMYSFAGYCSYLLAGGCQKRSFSIIGDFQNGKRVSLSVYLGEFFDIHLFVNGTVTQGDQRVSMPYASKGLYLETEAGYYKLSGEAYGFVARIDGSGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFMTQEGTLTSDPYDFANSWALSSGEQWCERASPPSSSCNISSGEMQKGLWEQCQLLKSTSVFARCHPLVDPEPFVALCEKTLCECAGGLECACPALLEYARTCAQEGMVLYGWTDHSACSPVCPAGMEYRQCVSPCARTCQSLHINEMCQERCVDGCSCPEGQLLDEGLCVESTECPCVHSGKRYPPGTSLSRDCNTCICRNSQWICSNEECPGECLVTGQSHFKSFDNRYFTFSGICQYLLARDCQDHSFSIVIETVQCADDRDAVCTRSVTVRLPGLHNSLVKLKHGAGVAMDGQDVQLPLLKGDLRIQHTVTASVRLSYGEDLQMDWDGRGRLLVKLSPVYAGKTCGLCGNYNGNQGDDFLTPSGLAEPRVEDFGNAWKLHGDCQDLQKQHSDPCALNPRMTRFSEEACAVLTSPTFEACHRAVSPLPYLRNCRYDVCSCSDGRECLCGALASYAAACAGRGVRVAWREPGRCELNCPKGQVYLQCGTPCNLTCRSLSYPDEECNEACLEGCFCPPGLYMDERGDCVPKAQCPCYYDGEIFQPEDIFSDHHTMCYCEDGFMHCTMSGVPGSLLPDAVLSSPLSHRSKRSLSCRPPMVKLVCPADNLRAEGLECTKTCQNYDLECMSMGCVSGCLCPPGMVRHENRCVALERCPCFHQGKEYAPGETVKIGCNTCVCQDRKWNCTDHVCDATCSTIGMAHYLTFDGLKYLFPGECQYVLVQDYCGSNPGTFRILVGNKGCSHPSVKCKKRVTILVEGGEIELFDGEVNVKRPMKDETHFEVVESGRYIILLLGKALSVVWDRHLSISVVLKQTYQEKVCGLCGNFDGIQNNDLTSSNLQVEEDPVDFGNSWKVSSQCADTRKVPLDSSPATCHNNIMKQTMVDSSCRILTSDVFQDCNKLVDPEPYLDVCIYDTCSCESIGDCACFCDTIAAYAHVCAQHGKVVTWRTATLCPQSCEERNLRENGYECEWRYNSCAPACQVTCQHPEPLACPVQCVEGCHAHCPPGKILDELLQTCVDPEDCPVCEVAGRRFASGKKVTLNPSDPEHCQICHCDVVNLTCEACQEPGGLVVPPTDAPVSPTTLYVEDISEPPLHDFYCSRLLDLVFLLDGSSRLSEAEFEVLKAFVVDMMERLRISQKWVRVAVVEYHDGSHAYIGLKDRKRPSELRRIASQVKYAGSQVASTSEVLKYTLFQIFSKIDRPEASRITLLLMASQEPQRMSRNFVRYVQGLKKKKVIVIPVGIGPHANLKQIRLIEKQAPENKAFVLSSVDELEQQRDEIVSYLCDLAPEAPPPTLPPDMAQVTVGPGLLGVSTLGPKRNSMVLDVAFVLEGSDKIGEADFNRSKEFMEEVIQRMDVGQDSIHVTVLQYSYMVTVEYPFSEAQSKGDILQRVREIRYQGGNRTNTGLALRYLSDHSFLVSQGDREQAPNLVYMVTGNPASDEIKRLPGDIQVVPIGVGPNANVQELERIGWPNAPILIQDFETLPREAPDLVLQRCCSGEGLQIPTLSPAPDCSQPLDVILLLDGSSSFPASYFDEMKSFAKAFISKANIGPRLTQVSVLQYGSITTIDVPWNVVPEKAHLLSLVDVMQREGGPSQIGDALGFAVRYLTSEMHGARPGASKAVVILVTDVSVDSVDAAADAARSNRVTVFPIGIGDRYDAAQLRILAGPAGDSNVVKLQRIEDLPTMVTLGNSFLHKLCSGFVRICMDEDGNEKRPGDVWTLPDQCHTVTCQPDGQTLLKSHRVNCDRGLRPSCPNSQSPVKVEETCGCRWTCPCVCTGSSTRHIVTFDGQNFKLTGSCSYVLFQNKEQDLEVILHNGACSPGARQGCMKSIEVKHSALSVELHSDMEVTVNGRLVSVPYVGGNMEVNVYGAIMHEVRFNHLGHIFTFTPQNNEFQLQLSPKTFASKTYGLCGICDENGANDFMLRDGTVTTDWKTLVQEWTVQRPGQTCQPILEEQCLVPDSSHCQVLLLPLFAECHKVLAPATFYAICQQDSCHQEQVCEVIASYAHLCRTNGVCVDWRTPDFCAMSCPPSLVYNHCEHGCPRHCDGNVSSCGDHPSEGCFCPPDKVMLEGSCVPEEACTQCIGEDGVQHQFLEAWVPDHQPCQICTCLSGRKVNCTTQPCPTAKAPTCGLCEVARLRQNADQCCPEYECVCDPVSCDLPPVPHCERGLQPTLTNPGECRPNFTCACRKEECKRVSPPSCPPHRLPTLRKTQCCDEYECACNCVNSTVSCPLGYLASTATNDCGCTTTTCLPDKVCVHRSTIYPVGQFWEEGCDVCTCTDMEDAVMGLRVAQCSQKPCEDSCRSGFTYVLHEGECCGRCLPSACEVVTGSPRGDSQSSWKSVGSQWASPENPCLINECVRVKEEVFIQQRNVSCPQLEVPVCPSGFQLSCKTSACCPSCRCERMEACMLNGTVIGPGKTVMIDVCTTCRCMVQVGVISGFKLECRKTTCNPCPLGYKEENNTGECCGRCLPTACTIQLRGGQIMTLKRDETLQDGCDTHFCKVNERGEYFWEKRVTGCPPFDEHKCLAEGGKIMKIPGTCCDTCEEPECNDITARLQYVKVGSCKSEVEVDIHYCQGKCASKAMYSIDINDVQDQCSCCSPTRTEPMQVALHCTNGSVVYHEVLNAMECKCSPRKCSK ats13_human A disintegrin and metalloproteinase with thrombospondin motifs 13 von Willebrand factor-cleaving protease ADAMTS13 C9orf8 UNQ6102/PRO20085 protein protein human Homo sapiens Human MHQRHPRARCPPLCVAGILACGFLLGCWGPSHFQQSCLQALEPQAVSSYLSPGAPLKGRPPSPGFQRQRQRQRRAAGGILHLELLVAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPSLGAQFRVHLVKMVILTEPEGAPNITANLTSSLLSVCGWSQTINPEDDTDPGHADLVLYITRFDLELPDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLGVTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASDGAAPRAGLAWSPCSRRQLLSLLSAGRARCVWDPPRPQPGSAGHPPDAQPGLYYSANEQCRVAFGPKAVACTFAREHLDMCQALSCHTDPLDQSSCSRLLVPLLDGTECGVEKWCSKGRCRSLVELTPIAAVHGRWSSWGPRSPCSRSCGGGVVTRRRQCNNPRPAFGGRACVGADLQAEMCNTQACEKTQLEFMSQQCARTDGQPLRSSPGGASFYHWGAAVPHSQGDALCRHMCRAIGESFIMKRGDSFLDGTRCMPSGPREDGTLSLCVSGSCRTFGCDGRMDSQQVWDRCQVCGGDNSTCSPRKGSFTAGRAREYVTFLTVTPNLTSVYIANHRPLFTHLAVRIGGRYVVAGKMSISPNTTYPSLLEDGRVEYRVALTEDRLPRLEEIRIWGPLQEDADIQVYRRYGEEYGNLTRPDITFTYFQPKPRQAWVWAAVRGPCSVSCGAGLRWVNYSCLDQARKELVETVQCQGSQQPPAWPEACVLEPCPPYWAVGDFGPCSASCGGGLRERPVRCVEAQGSLLKTLPPARCRAGAQQPAVALETCNPQPCPARWEVSEPSSCTSAGGAGLALENETCVPGADGLEAPVTEGPGSVDEKLPAPEPCVGMSCPPGWGHLDATSAGEKAPSPWGSIRTGAQAAHVWTPAAGSCSVSCGRGLMELRFLCMDSALRVPVQEELCGLASKPGSRREVCQAVPCPARWQYKLAACSVSCGRGVVRRILYCARAHGEDDGEEILLDTQCQGLPRPEPQEACSLEPCPPRWKVMSLGPCSASCGLGTARRSVACVQLDQGQDVEVDEAACAALVRPEASVPCLIADCTYRWHVGTWMECSVSCGDGIQRRRDTCLGPQAQAPVPADFCQHLPKPVTVRGCWAGPCVGQGTPSLVPHEEAAAPGRTTATPAGASLEWSQARGLLFSPAPQPRRLLPGPQENSVQSSACGRQHLEPTGTIDMRGPGQADCAVAIGRPLGEVVTLRVLESSLNCSAGDMLLLWGRLTWRKMCRKLLDMTFSSKTNTLVVRQRCGRPGGGVLLRYGSQLAPETFYRECDMQLFGPWGEIVSPSLSPATSNAGGCRLFINVAPHARIAIHALATNMGAGTEGANASYILIRDTHSLRTTAFHGQQVLYWESESSQAEMEFSEGFLKAQASLRGQYWTLQSWVPEMQDPQSWKGKEGT sspa_staau Glutamyl endopeptidase sspA Endoproteinase Glu-C Staphylococcal serine proteinase V8 protease V8 proteinase protein protein staau Staphylococcus aureus MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDAA w47sa5ys-1 2876527 N/A 2876531 unspecified role unspecified role bait bait regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P04275:p.Cys2773Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876532 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 2876527 Supp 4 2-RHS vwf-adamts13-1 2876527 N/A 2876531 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2876532 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false MDTCS_construct sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 2876527 Supp 4 1-LHS vwf-adamts13-2 2876527 N/A 2876531 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2876532 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 2876527 Supp 4 1-RHS vwf-adamts13-3 2876527 N/A 2876531 unspecified role unspecified role bait bait regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P04275:p.Cys2773Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false P04275:p.Gly1505Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876532 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 2876527 Supp 4 3-RHS vwf-adamts13-4 2876527 N/A 2876531 unspecified role unspecified role bait bait c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P04275:p.Gly1505Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P04275:p.Cys2773Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2876532 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false MDTCS_construct sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 2876527 Supp 4 3-LHS vwf-adamts13-5 2876527 N/A 2876531 unspecified role unspecified role bait bait regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P04275:p.Cys2773Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. N/A 2876532 unspecified role unspecified role prey prey Q76LX8:p.Glu225Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 2876527 Supp 4 4-RHS adamts13-vwf-2 2876528 N/A 2876532 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component P04275:p.Asn1610Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P04275:p.Glu1598Gly mutation mutation certain certain sequence position certain certain certain sequence position certain false P04275:p.Lys1617Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false IRDye QC-1 dye labelled dye label dye labelled certain certain sequence position certain certain certain sequence position certain false DyLight 633 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) protein cleavage protein cleavage false false false 1, Supp 1 increase in pH, but only for full length version of ADAMTS13 Full length ADAMTS13 showed maximum activity at pH6 and decreased activity at pH 7.4. The MDTCS construct (ADAMTS13 aas 1-684) maintained maximal activity at all pH values tested. adamts13-vwf-3 2876528 N/A 2876532 enzyme enzyme neutral component neutral component MDTCS_construct sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component P04275:p.Asn1610Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P04275:p.Glu1598Gly mutation mutation certain certain sequence position certain certain certain sequence position certain false P04275:p.Lys1617Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false IRDye QC-1 dye labelled dye label dye labelled certain certain sequence position certain certain certain sequence position certain false DyLight 633 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 2876598 2876593 protein cleavage protein cleavage false false false 1D, supp 1 increase in pH, but only for full length version of ADAMTS13 Full length ADAMTS13 showed maximum activity at pH6 and decreased activity at pH 7.4. The MDTCS construct (ADAMTS13 aas 1-684) maintained maximal activity at all pH values tested. adamts13-vwf-4 2876528 N/A 2876531 stimulator stimulator neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-plasma homo sapiens blood plasma plasma blood plasma N/A 2876531 stimulator stimulator neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-plasma homo sapiens blood plasma plasma blood plasma N/A 2876532 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component P04275:p.Asn1610Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P04275:p.Glu1598Gly mutation mutation certain certain sequence position certain certain certain sequence position certain false P04275:p.Lys1617Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false IRDye QC-1 dye labelled dye label dye labelled certain certain sequence position certain certain certain sequence position certain false DyLight 633 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 2876607 2876605 protein cleavage protein cleavage false false false 2D adamts13-vwf-5 2876528 N/A 2876532 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component P04275:p.Asn1610Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P04275:p.Glu1598Gly mutation mutation certain certain sequence position certain certain certain sequence position certain false P04275:p.Lys1617Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false IRDye QC-1 dye labelled dye label dye labelled certain certain sequence position certain certain certain sequence position certain false DyLight 633 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2876531 stimulator stimulator neutral component neutral component D4_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. protein cleavage protein cleavage false false false 2D Adding VWF cleavage fragments had little effect on ADAMTS13 activity. adamts13-vwf-6 2876529 N/A 2876532 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-plasma homo sapiens blood plasma plasma blood plasma N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-plasma homo sapiens blood plasma plasma blood plasma 2876634 2876632 protein cleavage protein cleavage false false false 3 full-length ADAMTS13 cleaved VWF ∼1.7-fold faster than MDTCS. adamts13-vwf-11 2876529 N/A 2876532 enzyme enzyme neutral component neutral component MDTCS_construct sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mouse_IgM_signal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n_terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-plasma homo sapiens blood plasma plasma blood plasma N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-plasma homo sapiens blood plasma plasma blood plasma 2876643 2876641 protein cleavage protein cleavage false false false 3i full-length ADAMTS13 cleaved VWF ∼1.7-fold faster than MDTCS. adamts13-vwf-10 2876530 N/A 2876533 enzyme enzyme neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-plasma homo sapiens blood plasma plasma blood plasma N/A 2876531 enzyme target enzyme target substrate substrate neutral component neutral component whole_molecule sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-plasma homo sapiens blood plasma plasma blood plasma 2876649 2876647 protein cleavage protein cleavage false false false Supp 3