DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mari-2014-1 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. DIP original experiment: DIP-264300X Structure (London, England : 1993) 2014 IMEx Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases. Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. Only protein-protein interactions imex curation mari-2014-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. DIP original experiment: DIP-264304X Structure (London, England : 1993) 2014 IMEx Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases. Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. Only protein-protein interactions imex curation mari-2014-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. DIP original experiment: DIP-264302X Structure (London, England : 1993) 2014 IMEx Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases. Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. Only protein-protein interactions imex curation mari-2014-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. DIP original experiment: DIP-264306X Structure (London, England : 1993) 2014 IMEx Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases. Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. Only protein-protein interactions imex curation mari-2014-5 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. DIP original experiment: DIP-264301X Structure (London, England : 1993) 2014 IMEx Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases. Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. Only protein-protein interactions imex curation mari-2014-6 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. DIP original experiment: DIP-264305X Structure (London, England : 1993) 2014 IMEx Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases. Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. Only protein-protein interactions imex curation mari-2014-7 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. DIP original experiment: DIP-264303X Structure (London, England : 1993) 2014 IMEx Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases. Mari S., Ruetalo N., Maspero E., Stoffregen MC., Pasqualato S., Polo S., Wiesner S. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG p46934-4 E3 ubiquitin-protein ligase NEDD4 NEDD4 KIAA0093 NEDD4-1 Cell proliferation-inducing gene 53 protein HECT-type E3 ubiquitin transferase NEDD4 Neural precursor cell expressed developmentally down-regulated protein 4 PIG53 protein protein human Homo sapiens Human MATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD Initiator Met-1 is removed. Contains a N-acetylalanine at position 2. ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG smuf2_human E3 ubiquitin-protein ligase SMURF2 SMURF2 SMAD ubiquitination regulatory factor 2 SMAD-specific E3 ubiquitin-protein ligase 2 HECT-type E3 ubiquitin transferase SMURF2 protein protein human Homo sapiens Human MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE scnng_rat Amiloride-sensitive sodium channel subunit gamma Scnn1g Epithelial Na(+) channel subunit gamma Gamma-NaCH Nonvoltage-gated sodium channel 1 subunit gamma SCNEG protein protein rat Rattus norvegicus (Rat) Rat MAPGEKIKAKIKKNLPVRGPQAPTIKDLMHWYCMNTNTHGCRRIVVSRGRLRRLLWIAFTLTAVALIIWQCALLVFSFYTVSVSIKVHFQKLDFPAVTICNINPYKYSAVSDLLTDLDSETKQALLSLYGVKESRKRREAGSMPSTLEGTPPRFFKLIPLLVFNENEKGKARDFFTGRKRKISGKIIHKASNVMHVHESKKLVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAEELLVTCFFDGMSCDARNFTLFHHPMYGNCYTFNNKENATILSTSMGGSEYGLQVILYINEDEYNPFLVSSTGAKVLIHQQNEYPFIEDVGMEIETAMSTSIGMHLTESFKLSEPYSQCTEDGSDVPVTNIYNAAYSLQICLYSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLYQAFVREELGCQSVCKQSCSFKEWTLTTSLAQWPSEASEKWLLNVLTWDQSQQINKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQWHKAKDWWARRQTPPSTETPSSRQGQDNPALDTDDDLPTFTSAMRLPPAPGSTVPGTPPPRYNTLRLDRAFSSQLTDTQLTNEL nedd4-hub-1 3271977 N/A 3271984 unspecified role unspecified role prey prey K63-Ub K63-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3271985 unspecified role unspecified role bait bait Region 565-900 Region 565-900 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P46934-4:p.Leu553Ala L553A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P46934-4:p.Tyr605Ala Y605A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P46934-4:p.Tyr634Ala Y634A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false bub-nedd4-1 3271978 N/A 3271986 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3271985 unspecified role unspecified role unspecified role unspecified role Region 565-900 Region 565-900 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false smurf2-hub-1 3271979 N/A 3271987 unspecified role unspecified role bait bait Region 414-748 Region 414-748 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3271984 unspecified role unspecified role prey prey K63-Ub K63-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bub-smurf2-1 3271980 N/A 3271986 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine N/A 3271987 unspecified role unspecified role unspecified role unspecified role Region 414-748 Region 414-748 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nedd4-bub-1 3271981 N/A 3271986 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine N/A 3271985 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 565-900 Region 565-900 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false nedd4-smurf2-1 3271982 N/A 3271987 unspecified role unspecified role unspecified role unspecified role Region 414-748 Region 414-748 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3271985 unspecified role unspecified role unspecified role unspecified role Region 1-152 Region 1-152 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false scnn1g-bub-1 3271983 N/A 3271988 unspecified role unspecified role bait bait Region 141-646 Region 141-646 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3271986 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine physical association physical association false false false