DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ guo-2014-12 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264678X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-13 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264668X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-14 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264676X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-15 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264670X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-16 caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264672X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-17 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264674X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-18 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264669X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-19 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264677X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-20 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264671X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-21 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264673X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation guo-2014-22 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. DIP original experiment: DIP-264675X Nature cell biology 2014 IMEx O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation. Guo B., Liang Q., Li L., Hu Z., Wu F., Zhang P., Ma Y., Zhao B., Kovács AL., Zhang Z., Feng D., Chen S., Zhang H. Only protein-protein interactions imex curation snp29_human Synaptosomal-associated protein 29 Vesicle-membrane fusion protein SNAP-29 Soluble 29 kDa NSF attachment protein SNAP29 protein protein human Homo sapiens Human MSAYPKSYNPFDDDGEDEGARPAPWRDARDLPDGPDAPADRQQYLRQEVLRRAEATAASTSRSLALMYESEKVGVASSEELARQRGVLERTEKMVDKMDQDLKISQKHINSIKSVFGGLVNYFKSKPVETPPEQNGTLTSQPNNRLKEAISTSKEQEAKYQASHPNLRKLDDTDPVPRGAGSAMSTDAYPKNPHLRAYHQKIDSNLDELSMGLGRLKDIALGMQTEIEEQDDILDRLTTKVDKLDVNIKSTERKVRQL stx6_human Syntaxin-6 STX6 protein protein human Homo sapiens Human MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL vamp8_human Vesicle-associated membrane protein 8 Endobrevin VAMP8 protein protein human Homo sapiens Human MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLICVIVFIIILFIVLFATGAFS stx17_human Syntaxin-17 STX17 protein protein human Homo sapiens Human MSEDEEKVKLRRLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHINAGRTVQQLRSNIREIEKLCLKVRKDDLVLLKRMIDPVKEEASAATAEFLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGAFHTTEAEASSQSLTQIYALPEIPQDQNAAESWETLEADLIELSQLVTDFSLLVNSQQEKIDSIADHVNSAAVNVEEGTKNLGKAAKYKLAALPVAGALIGGMVGGPIGLLAGFKVAGIAAALGGGVLGFTGGKLIQRKKQKMMEKLTSSCPDLPSQTDKKCS ogt1_human UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit O-linked N-acetylglucosamine transferase 110 kDa subunit O-GlcNAc transferase subunit p110 OGT protein protein human Homo sapiens Human MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA sna29_caeel Soluble NSF attachment protein 29 phi-28 K02D10.5 snap-29 protein protein caeel Caenorhabditis elegans MSRNPFDDDYRPSAASSTMPVKSYTTMGHYSGEDEADYYEREIEKTLQESLDSTERSRRHLENSEKIGTSTAQQLLEQREKLENTEKNLDEIHRTTQMTQRNLNSLKSFFGGMFKNKFTKKPQEPTETPTVPQSKSASRLSETATNLSSGGGSATFSGPSGQRTLTESSRSAIKGTRWEAMDNQIDENLDMMSANLRNLQRLGADLGKEVDSQNEMLDRIQYKAERNDGIVRDQDKQMQKILGTGASTSQTTAESLTPSMDTSTKMSLMMKATSLWK ogt1_caeel UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase K04G7.3 O-GlcNAc OGT ogt-1 protein protein caeel Caenorhabditis elegans MEKPNYFQSYNKVIGATGEQLAPGAVPPHPVLAPSIAPGGVAGVSAANMANIMQTPGFANLVQQAIRTQLENQAAQQLAVNQQFQLNGATAVQQQLLLTPQQSLAQPIALAPQPTVVLNGVSETLKKVTELAHRQFQSGNYVEAEKYCNLVFQSDPNNLPTLLLLSAINFQTKNLEKSMQYSMLAIKVNNQCAEAYSNLGNYYKEKGQLQDALENYKLAVKLKPEFIDAYINLAAALVSGGDLEQAVTAYFNALQINPDLYCVRSDLGNLLKAMGRLEEAKVCYLKAIETQPQFAVAWSNLGCVFNSQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVSAYLRALNLSGNHAVVHGNLACVYYEQGLIDLAIDTYKKAIDLQPHFPDAYCNLANALKEKGSVVEAEQMYMKALELCPTHADSQNNLANIKREQGKIEDATRLYLKALEIYPEFAAAHSNLASILQQQGKLNDAILHYKEAIRIAPTFADAYSNMGNTLKEMGDSSAAIACYNRAIQINPAFADAHSNLASIHKDAGNMAEAIQSYSTALKLKPDFPDAYCNLAHCHQIICDWNDYDKRVRKLVQIVEDQLCKKRLPSVHPHHSMLYPLSHAARIAIAAKHASLCFDKVHVQMLGKTPLIHADRFSVQNGQRLRIGYVSSDFGNHPTSHLMQSIPGMHDRSRVEVFCYALSVNDGTNFRSKLMNESEHFVDLSQIPCNGKAAEKIAQDGIHILINMNGYTKGARNEIFALRPAPIQVMWLGYPSTSGATFMDYIITDAVTSPLRLANAFTEKLAYMPHTFFIGDHAQMLRHLTDKVVVKDKETTERDSCLIMNTANMDPILAKSEIKEQVLDTEVVSGPNKELVRAEMVLPVLEVPTEPIKQMIMTGQMTMNVMEDMNVQNGLGQSQMHHKAATGEEIPNSVLLTSRAQYQLPDDAIVFCNFNQLYKIDPSTLDMWIKILENVPKSILWLLRFPYQGEEHIRKYCVERGLDPSRIVFSNVAAKEEHVRRGQLADVCLDTPLCNGHTTGMDILWTGTPMVTMPLESLASRVATSQLYALGVPELVAKTRQEYVSIAVRLGTDADHLANMRAKVWMARTSSTLFDVKQYCHDMEDLLGQMWKRYESGMPIDHITNNTETPHGL vamp4_human Vesicle-associated membrane protein 4 VAMP4 protein protein human Homo sapiens Human MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT snap29-stx6-1 3271567 N/A 3271578 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3271579 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false stx17-snap29-2 3271568 N/A 3271580 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3271578 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3271581 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false stx17-snap29-5 3271569 N/A 3271581 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3271578 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false ogt-snap29-1 3271570 N/A 3271578 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3271582 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false ogt_1-snap_29-1 3271571 N/A 3271583 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false caeel Caenorhabditis elegans N/A 3271584 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false caeel Caenorhabditis elegans physical association physical association false false false stx6-vamp4-1 3271572 N/A 3271579 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3271585 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false snap29-stx17-2 3271573 N/A 3271580 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3271578 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O95721:p.[Ser2Ala;Ser61Gly;Thr130Ala;Ser153Gly] S2A/S61G/T130A/S153G mutation increasing strength mutation increasing interaction strength mutation increasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3271581 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false stx6-snap29-1 3271574 N/A 3271578 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3271579 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false vamp8-snap29-1 3271575 N/A 3271580 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3271578 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false stx17-snap29-6 3271576 N/A 3271580 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3271578 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3271581 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false ogt-snap29-2 3271577 N/A 3271578 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3271582 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false