DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ korkhov-2014-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Korkhov VM., Mireku SA., Veprintsev DB., Locher KP. DIP original experiment: DIP-265543X Nature structural & molecular biology 2014 IMEx Structure of AMP-PNP-bound BtuCD and mechanism of ATP-powered vitamin B12 transport by BtuCD-F. Korkhov VM., Mireku SA., Veprintsev DB., Locher KP. Only protein-protein interactions imex curation korkhov-2014-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Korkhov VM., Mireku SA., Veprintsev DB., Locher KP. DIP original experiment: DIP-265546X Nature structural & molecular biology 2014 IMEx Structure of AMP-PNP-bound BtuCD and mechanism of ATP-powered vitamin B12 transport by BtuCD-F. Korkhov VM., Mireku SA., Veprintsev DB., Locher KP. Only protein-protein interactions imex curation korkhov-2014-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Korkhov VM., Mireku SA., Veprintsev DB., Locher KP. DIP original experiment: DIP-265544X Nature structural & molecular biology 2014 IMEx Structure of AMP-PNP-bound BtuCD and mechanism of ATP-powered vitamin B12 transport by BtuCD-F. Korkhov VM., Mireku SA., Veprintsev DB., Locher KP. Only protein-protein interactions imex curation korkhov-2014-4 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Korkhov VM., Mireku SA., Veprintsev DB., Locher KP. DIP original experiment: DIP-265545X Nature structural & molecular biology 2014 IMEx Structure of AMP-PNP-bound BtuCD and mechanism of ATP-powered vitamin B12 transport by BtuCD-F. Korkhov VM., Mireku SA., Veprintsev DB., Locher KP. Only protein-protein interactions imex curation btud_ecoli Vitamin B12 import ATP-binding protein BtuD btuD b1709 Vitamin B12-transporting ATPase JW1699 protein protein ecoli Escherichia coli (strain K12) MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLARMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFATPVWHYLTLHQHDKTRTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEPMNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRREEVLTPPNLAQAYGMNFRRLDIEGHRMLISTI btuc_ecoli Vitamin B12 import system permease protein BtuC btuC b1711 JW1701 protein protein ecoli Escherichia coli (strain K12) MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWFTPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLIAAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALMTWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGEISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLLPGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR btuf_ecoli Vitamin B12-binding protein yadT btuF b0158 JW0154 protein protein ecoli Escherichia coli (strain K12) MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGIKVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFLQFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGPDQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD btuc-btud-7 3271465 N/A 3271469 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3271470 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false btuc-btud-10 3271466 N/A 3271469 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3271470 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false btuc-btud-11 3271467 N/A 3271471 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3271469 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3271470 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false btuc-btud-12 3271468 N/A 3271471 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3271469 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3271470 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false