IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ deriziotis-2014-2 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. This experiment relates to a negative interaction The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Nature communications 2014 Only protein-protein interactions imex curation imex curation deriziotis-2014-4 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. This experiment relates to a negative interaction The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Nature communications 2014 Only protein-protein interactions imex curation imex curation deriziotis-2014-6 human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. This experiment relates to a negative interaction The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Nature communications 2014 Only protein-protein interactions imex curation imex curation o15409-6 Forkhead box protein P2 CAG repeat protein 44 Trinucleotide repeat-containing gene 10 protein FOXP2-S FOXP2 CAGH44 TNRC10 protein protein human Homo sapiens Human MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEVSTVELLHLQQQQALQAARQLLLQQQTSGLKSPKSSDKQRPLQVPVSVAMMTPQVITPQQMQQILQQQVLSPQQLQALLQQQQAVMLQQQQLQEFYKKQQEQLHLQLLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHPGKQAKEQQQQQQQQQQLAAQQLVFQQQLLQMQQLQQQQHLLSLQRQGLISIPPGQAALPVQSLPQAGLSPAEIQQLWKEVTGVHSMEDNGIKHGGLDLTTNNSSSTTSSNTSKASPPITHHSIVNGQSSVLSARRDSSSHEETGASHTLYGHGVCKWPGCESICEDFGQFLKHLNNEHALDDRSTAQCRVQMQVVQQLEIQLSKERERLQAMMTHLHMRPSEPKPSPKPVSAYCFINSK tbr1_human T-box brain protein 1 TES-56 TBR1 protein protein human Homo sapiens Human MQLEHCLSPSIMLSKKFLNVSSSYPHSGGSELVLHDHPIISTTDNLERSSPLKKITRGMTNQSDTDNFPDSKDSPGDVQRSKLSPVLDGVSELRHSFDGSAADRYLLSQSSQPQSAATAPSAMFPYPGQHGPAHPAFSIGSPSRYMAHHPVITNGAYNSLLSNSSPQGYPTAGYPYPQQYGHSYQGAPFYQFSSTQPGLVPGKAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNISGLDPTAHYNIFVDVILADPNHWRFQGGKWVPCGKADTNVQGNRVYMHPDSPNTGAHWMRQEISFGKLKLTNNKGASNNNGQMVVLQSLHKYQPRLHVVEVNEDGTEDTSQPGRVQTFTFPETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDTIYTGCDMDRLTPSPNDSPRSQIVPGARYAMAGSFLQDQFVSNYAKARFHPGAGAGPGPGTDRSVPHTNGLLSPQQAEDPGAPSPQRWFVTPANNRLDFAASAYDTATDFAGNAATLLSYAAAGVKALPLQAAGCTGRPLGYYADPSGWGARSPPQYCGTKSGSVLPCWPNSAAAAARMAGANPYLGEEAEGLAAERSPLPPGAAEDAKPKDLSDSSWIETPSSIKSIDSSDSGIYEQAKRRRISPADTPVSESSSPLKSEVLAQRDCEKNCAKDISGYYGFYSHS foxp2-tbr1-2 3317383 N/A 3317386 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3317387 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false true 6B-3 The interaction does not occur under these experimental conditions foxp2-tbr1-5 3317384 N/A 3317386 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3317387 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false true 6C-3 The interaction does not occur under these experimental conditions foxp2-tbr1-9 3317385 N/A 3317386 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3317387 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false true The interaction does not occur under these experimental conditions Supplementary fig 7