DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhang-2014-45 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262287X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-46 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262279X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-47 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262290X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-48 ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262286X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-49 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262282X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-50 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262293X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-51 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262280X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-52 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262291X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-53 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262288X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-54 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262292X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-55 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262281X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-56 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262283X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-57 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262295X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-58 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262294X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-59 ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262284X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-60 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262289X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation zhang-2014-61 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang Z., Wang S., Shen T., Chen J., Ding J. DIP original experiment: DIP-262285X Structure (London, England : 1993) 2014 IMEx Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2. Zhang Z., Wang S., Shen T., Chen J., Ding J. Only protein-protein interactions imex curation q8bpq7-1 Small G protein signaling modulator 1 Sgsm1 Rutbc2 RUN and TBC1 domain-containing protein 2 protein protein mouse Mus musculus Mouse MASVPAEAETRQRLLRTVKKEVKQIMEEAVTRKFVHEDSSHIISFCAAVEACVLHGLRRRAAGFLRSNKIAALFMKVGKGFPPAEELSRKVQELEQLIESARNQIQGLQENVRKLPKLPNLSPLAIKHLWIRTALFGRVLDKIVHYLVENSSKYYEKEALLMDPVDGPILASLLVGPCALEYTKMKTADHFWTDPSADELVQRHRIHSSHLRQDSPTKRPALCIQKRHSSGSMDDRPSISARDYVESLHQDSRATLLYGKNNVLVQPRDDMEAVPGYLSLHQTADVMTLKWTPNQLMNGSVGDLDYEKSVYWDYAVTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPKLRKRSPQGSSESTSSDKEDDEATDYVFRIIYPGTQSEFVPQDLMDVSMNNLPPLWQPSPRKSSCSSCSQSGSADGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGQGLTASIWEKYIQDSTTYPEQELLRLIYYGGVQPEIRRAVWPFLLGHYQFGMTEMERKEVDEQIHACYAQTMSEWLGCEAIVRQRERESHAAALAKCSSGASLDSHLHRMLHRDSTISNESSQSCSSGRQNLRLQSDSSSSTQVFESVDEVEQTEAEGRSEEKHPKIPNGNPANGTCSPDSGHPSSHNFSSGLSEHSEPSLSTEDSVLDAQRSLPAVFRPGDSSVEDGQSSEATTSRDEAPREELAVQDSLESDLLANESLEEFMSIPGSLDVALPEKDGAVMDGWPGEADKPSRADSEDNLSEEPEMESLFPALASLAVTSSANNEASPVSSSGVTYSPELLDLYTVNLHRIEKDVQRCDRSYWYFTAANLEKLRNIMCSYIWQHIEIGYVQGMCDLLAPLLVILDDEALAFSCFTELMKRMNQNFPHGGAMDTHFANMRSLIQILDSELFELMHQNGDYTHFYFCYRWFLLDFKRELVYDDVFSVWETIWAAKHVSSAHYVLFIALALVEVYRDIILENNMDFTDIIKFFNEMAERHNAKQILQLARDLVHKVQILIENK rab9a_mouse Ras-related protein Rab-9A Rab9a Rab9 Sid99 Sid 99 protein protein mouse Mus musculus Mouse MAGKSSLFKIILLGDGGVGKSSLMNRYVTNKFDSQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPESFPFVILGNKTDIKERQVSTEEAQAWCKDNGDYPYFETSAKDSTNVAAAFEEAVRRILATEDRSEHLIQTDTVNLHRKPKPNSSCC rab9b_mouse Ras-related protein Rab-9B Rab9b protein protein mouse Mus musculus Mouse MSGKSLLLKVILLGDGGVGKSSLMNRYVTNKFDSQAFHTIGVEFLNRDLEVDGRFVTLQIWDTAGQERFKSLRTPFYRGADCCLLTFSVDDRQSFENLGNWQKEFIYYADVKDPDHFPFVVLGNKVDKEDRQVTTEEAQAWCMENGNYPYLETSAKDDTNVTVAFEEAVRQVLAVEEQLEHCMLGHTIDLNSGSKASSSCC q80u12-1 Small G protein signaling modulator 2 Sgsm2 Kiaa0397 Rutbc1 RUN and TBC1 domain-containing protein 1 protein protein mouse Mus musculus Mouse MGSAEDAVKEKLLWNVKKEVKQIMEEAVTRKFVHEDSSHIIALCGAVEACLLHQLRRRAAGFLRSDKMAALFTKVGKTCPVAEDICHKVQELQQQAEGRKPSGGSQEALRKQGSTGGKAPALSPQALKHIWVRTALMEKVLDRVVQYLAENCSKYYEKEALLADPVFGPILACLLVGPCALEYTKLKTADHYWTDPSADELVQRHRIRGPPNRQDSPAKRPALGIRKRHSSGSASEDRLAACAREYVESLHQNSRTRLLYGKNNVLVQPKEDMEAVPGYLSLHQSAENLTLKWTPNQLMNGTLGDSELEKSVYWDYALVVPFSQIVCIHCHQQKSGGTLVLVSQDGIQRPPLHFPQGGHLLSFLSCLENGLLPRGQLEPPLWTQQGKGKVFPKLRKRSSIRSIDVEELGVGRATDYVFRIIYPGHRHEHNAGDMIEMQGFGPSLTAWHLEPLCSQGSSCLSCSSSSSPYATPSHCSCIPDRLPLRLLCESMKRQIVSRAFYGWLAYCRHLSTVRTHLSALVHHNIIPPDRPPGASGGLTKDVWSKYQKDEKNYKELELLRQVYYGGVEHEIRKDVWPFLLGHYKFGMSKKEMEQVDTAVAARYQQVLAEWKACEVVVRQREREAHPATLTKFSSGSSIDSHVQRLVHRDSTISNDVFISVDDLEPSGPQDLEDSKPKREQEPGAGTPGIAAAEQQSVEFDSPDSGLPSSRNYSVASGIQSSLDEAQSVGFEDDGAGEDGSEGPATAAHTFPGPHDPGQETLAPASELEAGQELAAVCAAAYTIELLDTVALNLHRIDKDVQRCDRNYWYFTTSNLERLRDIMCSYVWEHLDMGYVQGMCDLLAPLLVILDNDQLAYSCFSHLMKRMGQNFPSGGAMDSHFANMRSLIQILDSELFELMHQNGDYTHFYFCYRWFLLDFKRELLYEDVFAVWEVIWAARRISSEHFVLFIALALVEAYREIIRDNNMDFTDIIKFFNERAERHDAQEILRIARDLVHKVQMLIDNK sgsm1-rab9a-1 3270017 N/A 3270034 unspecified role unspecified role bait bait Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role prey prey Region 1-171 Region 1-171 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false rab9a-sgsm1-1 3270018 N/A 3270034 unspecified role unspecified role unspecified role unspecified role Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role unspecified role unspecified role Region 1-199 Region 1-199 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sgsm1-rab9b-1 3270019 N/A 3270036 unspecified role unspecified role prey prey Region 1-171 Region 1-171 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3270034 unspecified role unspecified role bait bait Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false rab9b-1 3270020 N/A 3270036 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rab9a-sgsm1-2 3270021 N/A 3270034 unspecified role unspecified role prey prey Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8BPQ7-1:p.Leu256Ala L256A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Lys260Ala K260A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Asn261Ala N261A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Tyr277Ala Y277A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Asn294Ala N294A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Met297Ala M297A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role bait bait Region 1-199 Region 1-199 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false rab9a-sgsm1-3 3270022 N/A 3270034 unspecified role unspecified role unspecified role unspecified role Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role unspecified role unspecified role Region 1-171 Region 1-171 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Ile40Ala I40A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Gly41Ala G41A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Gly41Asp G41D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Val42Ala V42A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Glu43Ala E43A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Phe44Ala F44A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Trp61Ala W61A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Phe76Ala F76A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rab9a-sgsm1-4 3270023 N/A 3270034 unspecified role unspecified role unspecified role unspecified role Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role unspecified role unspecified role Region 1-199 Region 1-199 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sgsm2-rab9a-1 3270024 N/A 3270037 unspecified role unspecified role bait bait Region 242-424 Region 242-424 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role prey prey Region 1-171 Region 1-171 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false sgsm1-rab9b-2 3270025 N/A 3270036 unspecified role unspecified role prey prey Region 1-171 Region 1-171 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3270034 unspecified role unspecified role bait bait Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false sgsm2-rab9b-1 3270026 N/A 3270036 unspecified role unspecified role prey prey Region 1-171 Region 1-171 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3270037 unspecified role unspecified role bait bait Region 242-424 Region 242-424 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false rab9a-sgsm1-5 3270027 N/A 3270034 unspecified role unspecified role prey prey Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role bait bait Region 1-199 Region 1-199 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9R0M6:p.Ile40Ala I40A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Gly41Ala G41A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Gly41Asp G41D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Val42Ala V42A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Glu43Ala E43A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Phe44Ala F44A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Trp61Ala W61A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Phe69Ala F69A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Leu72Ala L72A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9R0M6:p.Phe76Ala F76A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rab9a-1 3270028 N/A 3270035 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false rab9b-sgsm1-1 3270029 N/A 3270034 unspecified role unspecified role unspecified role unspecified role Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3270036 unspecified role unspecified role unspecified role unspecified role Region 1-171 Region 1-171 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rab9a-sgsm1-6 3270030 N/A 3270034 unspecified role unspecified role unspecified role unspecified role Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Leu256Ala L256A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Lys260Ala K260A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Asn261Ala N261A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Tyr277Ala Y277A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Asn294Ala N294A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BPQ7-1:p.Met297Ala M297A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role unspecified role unspecified role Region 1-199 Region 1-199 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rab9a-2 3270031 N/A 3270035 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sgsm1-rab9a-2 3270032 N/A 3270034 unspecified role unspecified role bait bait Region 254-425 Region 254-425 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3270035 unspecified role unspecified role prey prey Region 1-171 Region 1-171 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false rab9b-2 3270033 N/A 3270036 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false