IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hiraishi-2013-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hiraishi H., Oatman J., Haller SL., Blunk L., McGivern B., Morris J., Papadopoulos E., Gutierrez W., Gordon M., Bokhari W., Ikeda Y., Miles D., Fellers J., Asano M., Wagner G., Tazi L., Rothenburg S., Brown SJ., Asano K. Details about eIF2-beta construct were given by the authors upon email request. kasano@ksu.edu Nucleic acids research 2013 Only protein-protein interactions imex curation imex curation hiraishi-2013-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Hiraishi H., Oatman J., Haller SL., Blunk L., McGivern B., Morris J., Papadopoulos E., Gutierrez W., Gordon M., Bokhari W., Ikeda Y., Miles D., Fellers J., Asano M., Wagner G., Tazi L., Rothenburg S., Brown SJ., Asano K. Details about eIF2-beta construct were given by the authors upon email request. kasano@ksu.edu Nucleic acids research 2013 Only protein-protein interactions imex curation imex curation eif3g_yeast Eukaryotic translation initiation factor 3 subunit G TIF35 YDR429C D9461.16 Eukaryotic translation initiation factor 3 RNA-binding subunit Translation initiation factor eIF3 p33 subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEVAPEEIIENADGSRSIITYKIEDGVKYKITQKVKEVKVLEKVHKSVAERKNWHKYGSEKGSPAGPSAVTARLGEEVELRLSRNWKQAEEERIQKEKASLTKTGLQCRLCGNDHMTMNCPFKTILSELSALEDPATNEGGVEAASEEKAGQVGGAGSIPGQYVPPSRRAGARDPSSDAYRDSRERDDMCTLKIMQVNENADENSLREELLFPFAPIPRVSVVRNKETGKSRGLAFVTFSSEEVAEQALRFLDGRGYMNLILRVEWSKPKVKE 340A5285F2C84E06 if2g_yeast Eukaryotic translation initiation factor 2 subunit gamma YER025W GCD11 TIF213 SUI4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLQDQEPSIIINGNLEPVGEPDIVEETEVVAQETQETQDADKPKKKVAFTGLEEDGETEEEKRKREFEEGGGLPEQPLNPDFSKLNPLSAEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCQEPTCPEPDCYRSFKSDKEISPKCQRPGCPGRYKLVRHVSFVDCPGHDILMSTMLSGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHVIILQNKVDLMREESALEHQKSILKFIRGTIADGAPIVPISAQLKYNIDAVNEFIVKTIPVPPRDFMISPRLIVIRSFDVNKPGAEIEDLKGGVAGGSILNGVFKLGDEIEIRPGIVTKDDKGKIQCKPIFSNIVSLFAEQNDLKFAVPGGLIGVGTKVDPTLCRADRLVGQVVGAKGHLPNIYTDIEINYFLLRRLLGVKTDGQKQAKVRKLEPNEVLMVNIGSTATGARVVAVKADMARLQLTSPACTEINEKIALSRRIEKHWRLIGWATIKKGTTLEPIA D498AE62BC3E81CD m4md31_trica 5MP protein protein red flour beetle Tribolium castaneum Red flour beetle MSQKVEKPVLSGQRIKTRKRDEKERYDPVGFRDAIISGLDQCNNNFEAISRFLDGAGNKQDYRRYGESLFDILIAGGILVPGGTLLQEGEAPFKTSACIFNSPDEINLETMKSWEQVFIKLMRRYKYLEKIFQDEIKKVLMFVKYFTPAERKKLSVMVYLWISNGSIPFSAVLVLSNSHLVKDHLALDFLIDVFKYWRQDKGVNSLYSAIKKSNIESHLMSFVPDTKQSQVYFRNAFQENGLEEILKLYNDQHQQLAKKELQVMLADSPSENKPQRDIIAELREFAAKENIQEHETVCIIWTTLMDIPEWSKKEELVTDQAVRHLKLYTSLFSAFTQSARSELNLLLKVQEYCCSNMTFMRAFQKIIMLFYKTDVISEQVILKWYKQDYSVKGKMMFVDQMKQFVEWLQNAEEESDSEGSTE k7tu45_wheat 5MP protein protein wheat Triticum aestivum (Wheat) MSSKEKPTLGGQRIKTRKRNIAAPSDPSSFSDAIVQTYLDNAGDLELVAKSIESSDLNFSRYGDTFFEVVFIGVRTQPGTIKPEEEGERHPYSLIDCAAQREAILPYVLFIQKTLRRRPFLIKSLENVMRKFLQSLEFFEENERQKLAIFTALAFSQKLSGLPPETVFQPLLKDNLVAKGIVLSFITEFFKEYLKENSLDDLIALLKKGKMEDNLLEFFPSGKRTSEALSEHFTKEGLTSLVEYNSKKMFEVKLKEIKSTLTTMINEEAEISEVTEVVKQQVKDAKFPDIEVVRMLWDVLMEAVQWSGKNQQQNSNSALRQVNAWAGLMNAFCTSGKLELELIYKVQTQCYEDAKLMKLFPEIIRSLYDQDVLAEDTILLWFRKGSNQKGRQSFVRALEPFVKWLEEAEEEE t2esu9_9basi 5MP protein protein Puccinia triticina Puccinia triticina MSAAAAAKPSLAGVRNKQRKGVAKAAAKFEPEEFRDQILKHLSTLPPNDLDAVAAKLDTLGNQLDYRKYEGPLFEVLLVGGILAPGGSFVDDGAERSPFSILTSTQYPIDQADMKKHVDVFNKLIRRYKYLQKIFEENALTGLLQYINKFEGPDLEKLAYSLALFMSTALTSSNVLLTLQKEHLVKDGVSLNFITKVFTAYLQVESADHLSGALRRGGVVDMYGFFPPHKREPSDLEAHFKAEKLDAIVAYAHKQRSGQVRDETLVRLREMVASEDPQSEIISFLQEQAQSGLIPDVDFIALVWHGLMDRLDMGSRPDQIVDMVVRAVVAVAPVLAAFCKNARTQISMINTIQVWAYENTKVMGAFAKIIKELYAHDALSEQAILYWHSKGSKAQARQHFLKSTEPLVKFLKEQDEDDDDDE m1r6y5_giain 5MP DHA2_7522 protein protein giain Giardia intestinalis MSKPLPGALADTKIRTRKRNIVVQKDPESFLEALEHLFVGDSLEEVFKNLDSATDIDYKTYHEFFFDRFISGSIGVCFGRVDKRKTPRSPSIFADSLSKVDAWISILERFMRKRPYMRIGIGEVIGRAMLTIDNIPQYLDHVMSICSHLANLDILKYEKTTEFFLLKLLRINHLVVSGEALSILSKFTNLYATLSSQQKAWEYLIAGEVVDKLFDFCPEHCSDVLKLLEDSYGMQWFVELYRTNQTKNNITTWEQKIIQFMQKDPEGEEETLEALREDALRQVKQCKEECGMSAEESVMFMWRCIVSLSKTMSKDIVGALKKWGPCLYTLVGDDLVMQGKLLSTVQTTCYNNPNIINSFKPIVVQLYDLEILEADVIIAWANGNSGPGRSLFGEQLAEFVTWLEADDGED if2b_human Eukaryotic translation initiation factor 2 subunit 2 Eukaryotic translation initiation factor 2 subunit beta EIF2S2 EIF2B protein protein human Homo sapiens Human MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN bzw2_human Basic leucine zipper and W2 domain-containing protein 2 BZW2 HSPC028 MSTP017 protein protein human Homo sapiens Human MNKHQKPVLTGQRFKTRKRDEKEKFEPTVFRDTLVQGLNEAGDDLEAVAKFLDSTGSRLDYRRYADTLFDILVAGSMLAPGGTRIDDGDKTKMTNHCVFSANEDHETIRNYAQVFNKLIRRYKYLEKAFEDEMKKLLLFLKAFSETEQTKLAMLSGILLGNGTLPATILTSLFTDSLVKEGIAASFAVKLFKAWMAEKDANSVTSSLRKANLDKRLLELFPVNRQSVDHFAKYFTDAGLKELSDFLRVQQSLGTRKELQKELQERLSQECPIKEVVLYVKEEMKRNDLPETAVIGLLWTCIMNAVEWNKKEELVAEQALKHLKQYAPLLAVFSSQGQSELILLQKVQEYCYDNIHFMKAFQKIVVLFYKADVLSEEAILKWYKEAHVAKGKSVFLDQMKKFVEWLQNAEEESESEGEEN 5mp-gcd11-1 2871601 N/A 2871603 unspecified role unspecified role prey prey N/A 2871604 unspecified role unspecified role prey prey N/A 5MP 2871605 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2B, lane 4 5mp-gcd11-2 2871601 N/A 2871603 unspecified role unspecified role prey prey N/A 2871604 unspecified role unspecified role prey prey N/A 5MP 2871606 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2B, lane 5 5mp-gcd11-3 2871601 N/A 2871603 unspecified role unspecified role prey prey N/A 2871604 unspecified role unspecified role prey prey N/A 5MP 2871607 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2B, lane 6 5mp-gcd11-4 2871601 N/A 2871604 unspecified role unspecified role prey prey N/A 5MP 2871608 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2B, lane 7 bzw2-eif2s2-1 2871602 N/A eIF2-beta 2871609 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false gb1 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 5MP1 2871610 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false 2871602 5A bzw2-eif2s2-2 2871602 N/A eIF2-beta 2871609 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false gb1 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 5MP1-CTD 2871610 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false CTD fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false 2871602 5B