HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html somarajan-2014-2 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Somarajan SR., Al-Asadi F., Ramasamy K., Pandranki L., Baseman JB., Kannan TR. Kannan@uthscsa.edu mBio 2014 imex curation Only protein-protein interactions imex curation somarajan-2014-3 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Somarajan SR., Al-Asadi F., Ramasamy K., Pandranki L., Baseman JB., Kannan TR. Origin of ANXA2 inferred from previous work of contributing author then traced through clone designation (http://www.sourcebioscience.com) Kannan@uthscsa.edu mBio 2014 imex curation Only protein-protein interactions imex curation somarajan-2014-1 in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Somarajan SR., Al-Asadi F., Ramasamy K., Pandranki L., Baseman JB., Kannan TR. Kannan@uthscsa.edu mBio 2014 imex curation Only protein-protein interactions imex curation somarajan-2014-4 human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Somarajan SR., Al-Asadi F., Ramasamy K., Pandranki L., Baseman JB., Kannan TR. Kannan@uthscsa.edu mBio 2014 imex curation Only protein-protein interactions imex curation cards_mycpn ADP-ribosylating toxin CARDS CARDX TX ADP-ribosyltransferase CARDS MP464 MPN_372 protein protein mycpn Mycoplasma pneumoniae (strain ATCC 29342 / M129) MPNPVRFVYRVDLRSPEEIFEHGFSTLGDVRNFFEHILSTNFGRSYFISTSETPTAAIRFFGSWLREYVPEHPRRAYLYEIRADQHFYNARATGENLLDLMRQRQVVFDSGDREMAQMGIRALRTSFAYQREWFTDGPIAAANVRSAWLVDAVPVEPGHAHHPAGRVVETTRINEPEMHNPHYQELQTQANDQPWLPTPGIATPVHLSIPQAASVADVSEGTSASLSFACPDWSPPSSNGENPLDKCIAEKIDNYNLQSLPQYASSVKELEDTPVYLRGIKTQKTFMLQADPQNNNVFLVEVNPKQKSSFPQTIFFWDVYQRICLKDLTGAQISLSLTAFTTQYAGQLKVHLSVSAVNAVNQKWKMTPQDIAITQFRVSSELLGQTENGLFWNTKSGGSQHDLYVCPLKNPPSDLEELQIIVDECTTHAQFVTMRAASTFFVDVQLGWYWRGYYYTPQLSGWSYQMKTPDGQIFYDLKTSKIFFVQDNQNVFFLHNKLNKQTGYSWDWVEWLKHDMNEDKDENFKWYFSRDDLTIPSVEGLNFRHIRCYADNQQLKVIISGSRWGGWYSTYDKVESNVEDKILVKDGFDRF anxa2_human Annexin A2 ANXA2 ANX2 ANX2L4 CAL1H LPC2D Annexin-2 Annexin II Lipocortin II Calpactin I heavy chain Chromobindin-8 p36 Protein I Placental anticoagulant protein IV Calpactin-1 heavy chain protein protein human Homo sapiens Human MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD cards_mycpn-anxa2-1 2955814 N/A 2955818 unspecified role unspecified role prey prey n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 2955819 unspecified role unspecified role bait bait physical association physical association false false false 1B cards_mycpn-anxa2-2 2955814 N/A 2955818 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) N/A 2955819 unspecified role unspecified role bait bait n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2A cards_mycpn-anxa2-3 2955815 N/A 2955818 unspecified role unspecified role prey prey purified purified n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 2955819 unspecified role unspecified role bait bait purified purified n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false 3A anxa2-cards_mycpn-2 2955815 N/A 2955818 unspecified role unspecified role prey prey purified purified n terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 2955819 unspecified role unspecified role bait bait purified purified n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false 2B & 3B Truncated AnxA2 proteins (AnxA2267 (amino acids 1 to 267) and 268AnxA2 (amino acids 268 to 339) demonstrated higher binding to CARDS than when using the full AnxA2. cards-anxa2-1 2955816 N/A 2955818 unspecified role unspecified role bait bait n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 2955819 unspecified role unspecified role prey prey human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma physical association physical association false false false 1A cards-anxa2-2 2955817 N/A 2955819 unspecified role unspecified role neutral component neutral component N/A 2955818 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5