DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ romano-2014-4 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266976X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266981X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-6 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266986X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-7 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266979X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-8 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266984X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266977X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-10 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266980X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-11 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266982X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-12 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266978X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-13 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266983X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation romano-2014-14 in vitro In vitro peptide array peptide array autoradiography autoradiography Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266985X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions imex curation stk3_human Serine/threonine-protein kinase 3 STE20-like kinase MST2 Mammalian STE20-like protein kinase 2 Serine/threonine-protein kinase Krs-1 STK3 MST2 KRS1 protein protein human Homo sapiens Human MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF stk3_mouse Serine/threonine-protein kinase 3 Mst2 Stk3 Mess1 Mammalian STE20-like protein kinase 2 STE20-like kinase MST2 protein protein mouse Mus musculus Mouse MEQPPASKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKSPEQRATATQLLQHPFIKNAKPVSILRDLIAEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSSESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEEEEEEEEEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCDQSMREPGPMSNSVFPDNWRVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELHQRYSAKRQPILDAMDAKKRRQQNF rash_human GTPase HRas HRAS HRAS1 Transforming protein p21 p21ras H-Ras-1 c-H-ras Ha-Ras protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS ksr1_human Kinase suppressor of Ras 1 KSR1 KSR protein protein human Homo sapiens Human MDRAALRAAAMGEKKEGGGGGDAAAAEGGAGAAASRALQQCGQLQKLIDISIGSLRGLRTKCAVSNDLTQQEIRTLEAKLVRYICKQRQCKLSVAPGERTPELNSYPRFSDWLYTFNVRPEVVQEIPRDLTLDALLEMNEAKVKETLRRCGASGDECGRLQYALTCLRKVTGLGGEHKEDSSWSSLDARRESGSGPSTDTLSAASLPWPPGSSQLGRAGNSAQGPRSISVSALPASDSPTPSFSEGLSDTCIPLHASGRLTPRALHSFITPPTTPQLRRHTKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVSSMRFDLSHGSPQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDSRFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAAPLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIVREMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIGSGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLNRRLSHPGHFWKSADINSSKVVPRFERFGLGVLESSNPKM raf1_mouse RAF proto-oncogene serine/threonine-protein kinase Raf1 Craf Proto-oncogene c-RAF Raf-1 protein protein mouse Mus musculus Mouse MEHIQGAWKTISNGFGLKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDSGVPAPPSFPMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLHVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDDNPFSFQSDVYSYGIVLYELMAGELPYAHINNRDQIIFMVGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF raf1-stk3-1 3266884 N/A 3266895 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266896 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false raf1-stk3-2 3266885 N/A 3266895 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266896 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false raf1-stk3-3 3266886 N/A 3266897 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3266896 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse physical association physical association false false false hras-raf1-8 3266887 N/A 3266898 unspecified role unspecified role bait bait Au5-tag Au5-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3266896 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false raf1-ksr1-1 3266888 N/A 3266899 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266896 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false stk3-raf1-1 3266889 N/A 3266895 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3266896 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false stk3-raf1-2 3266890 N/A 3266900 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3266897 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false stk3-raf1-3 3266891 N/A 3266895 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3266896 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false raf1-hras-11 3266892 N/A 3266898 unspecified role unspecified role prey prey Au5-tag Au5-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3266896 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false raf1-stk3-4 3266893 N/A 3266895 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3266896 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false raf1-stk3-5 3266894 N/A 3266895 unspecified role unspecified role prey prey Region 140-170 Region 140-170 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 40-80 Region 40-80 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 86-116 Region 86-116 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 381-416 Region 381-416 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 3266896 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false