DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ follis-2014-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260914X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260912X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-3 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260920X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260916X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260918X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-6 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260919X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260921X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-8 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260913X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260915X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation follis-2014-10 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. DIP original experiment: DIP-260917X Nature structural & molecular biology 2014 IMEx The DNA-binding domain mediates both nuclear and cytosolic functions of p53. Follis AV., Llambi F., Ou L., Baran K., Green DR., Kriwacki RW. Only protein-protein interactions imex curation q07817-1 Bcl-2-like protein 1 BCLX Apoptosis regulator Bcl-X BCL2L1 BCL2L Bcl-xL protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG bid_human BH3-interacting domain death agonist p22 BID BID protein protein human Homo sapiens Human MDCEVNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSLLRDVFHTTVNFINQNLRTYVRSLARNGMD bcl2l1-tp53-6 3266240 N/A 3266250 unspecified role unspecified role unspecified role unspecified role Region 1-211 Region 1-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266251 unspecified role unspecified role unspecified role unspecified role Region 1-102 Region 1-102 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 102-312 Region 102-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bcl2l1-tp53-9 3266241 N/A 3266250 unspecified role unspecified role unspecified role unspecified role Region 1-211 Region 1-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266251 unspecified role unspecified role unspecified role unspecified role Region 1-102 Region 1-102 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bcl2l1-tp53-10 3266242 N/A 3266250 unspecified role unspecified role unspecified role unspecified role FLuorescein-label FLuorescein-label fluorescein fluorescein label fluorescein certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266251 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false bcl2l1-bax-8 3266243 N/A 3266252 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266251 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266250 unspecified role unspecified role bait bait CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false bcl2l1-bax-9 3266244 N/A 3266252 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266250 unspecified role unspecified role bait bait CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false bcl2l1-tp53-11 3266245 N/A 3266250 unspecified role unspecified role unspecified role unspecified role FLuorescein-label FLuorescein-label fluorescein fluorescein label fluorescein certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266251 unspecified role unspecified role unspecified role unspecified role Region 1-360 Region 1-360 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P04637:p.Lys120Glu K120E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.[His178Asp;Glu180Lys] H178D/E180K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bcl2l1-bid-5 3266246 N/A 3266253 unspecified role unspecified role unspecified role unspecified role Region 79-103 Region 79-103 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3266250 unspecified role unspecified role unspecified role unspecified role Region 1-211 Region 1-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bcl2l1-tp53-12 3266247 N/A 3266250 unspecified role unspecified role unspecified role unspecified role Region 1-211 Region 1-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266251 unspecified role unspecified role unspecified role unspecified role Region 102-312 Region 102-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tp53-bcl2l1-4 3266248 N/A 3266250 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266251 unspecified role unspecified role bait bait P04637:p.Lys120Glu K120E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.Glu180Lys E180K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.Asp186Ala D168A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P04637:p.[His178Asp;Glu180Lys] H178D/E180K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false bcl2l1-tp53-13 3266249 N/A 3266251 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266250 unspecified role unspecified role bait bait CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false