DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ guilvout-2014-1 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. DIP original experiment: DIP-260619X Structure (London, England : 1993) 2014 IMEx Independent domain assembly in a trapped folding intermediate of multimeric outer membrane secretins. Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. Only protein-protein interactions imex curation guilvout-2014-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. DIP original experiment: DIP-260623X Structure (London, England : 1993) 2014 IMEx Independent domain assembly in a trapped folding intermediate of multimeric outer membrane secretins. Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. Only protein-protein interactions imex curation guilvout-2014-3 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. DIP original experiment: DIP-260620X Structure (London, England : 1993) 2014 IMEx Independent domain assembly in a trapped folding intermediate of multimeric outer membrane secretins. Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. Only protein-protein interactions imex curation guilvout-2014-4 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. DIP original experiment: DIP-260621X Structure (London, England : 1993) 2014 IMEx Independent domain assembly in a trapped folding intermediate of multimeric outer membrane secretins. Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. Only protein-protein interactions imex curation guilvout-2014-5 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. DIP original experiment: DIP-260622X Structure (London, England : 1993) 2014 IMEx Independent domain assembly in a trapped folding intermediate of multimeric outer membrane secretins. Guilvout I., Chami M., Disconzi E., Bayan N., Pugsley AP., Huysmans GH. Only protein-protein interactions imex curation gspd_pseae Secretin XcpQ xcpQ General secretion pathway protein D Type II secretion system protein D PA3105 protein protein pseae Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) MSQPLLRALFAPSSRSYVPAVLLSLALGIQAAHAENSGGNAFVPAGNQQEAHWTINLKDADIREFIDQISEITGETFVVDPRVKGQVSVVSKAQLSLSEVYQLFLSVMSTHGFTVVAQGDQARIVPNAEAKTEAGGGQSAPDRLETRVIQVQQSPVSELIPLIRPLVPQYGHLAAVPSANALIISDRSANIARIEDVIRQLDQKGSHDYSVINLRYGWVMDAAEVLNNAMSRGQAKGAAGAQVIADARTNRLIILGPPQARAKLVQLAQSLDTPTARSANTRVIRLRHNDAKTLAETLGQISEGMKNNGGQGGEQTGGGRPSNILIRADESTNALVLLADPDTVNALEDIVRQLDVPRAQVLVEAAIVEISGDIQDAVGVQWAINKGGMGGTKTNFANTGLSIGTLLQSLESNKAPESIPDGAIVGIGSSSFGALVTALSANTKSNLLSTPSLLTLDNQKAEILVGQNVPFQTGSYTTNSEGSSNPFTTVERKDIGVSLKVTPHINDGAALRLEIEQEISALLPNAQQRNNTDLITSKRSIKSTILAENGQVIVIGGLIQDDVSQAESKVPLLGDIPLLGRLFRSTKDTHTKRNLMVFLRPTVVRDSAGLAALSGKKYSDIRVIDGTRGPEGRPSILPTNANQLFDGQAVDLRELMTE a0a0h3h6n4_kleok KOX_11310 protein protein kleok Klebsiella oxytoca (strain ATCC 8724 / DSM 4798 / JCM 20051 / NBRC 3318 / NRRL B-199 / KCTC 1686) MIIANVIRPFSLMLLVFAALLFKPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIGDEVVTRVVPLTNVAARDLAPLLRQLNDNAGAGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDRSVVTVPLAWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRIIAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAAKPVAALDKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRDRDEYRQASSGQYTAFNDAQSKQRGKENNDAMLSNDLLEIYPRQDTAAFRQVSAAIDAFNLGGNL xcpq-1 3294686 N/A 3294691 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction false false false puld-1 3294687 N/A 3294692 unspecified role unspecified role unspecified role unspecified role Region 28-42/259-660 Region 28-42/259-660 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false xcpq-2 3294688 N/A 3294691 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction false false false puld-2 3294689 N/A 3294692 unspecified role unspecified role unspecified role unspecified role Region 28-42/259-660 Region 28-42/259-660 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false puld-3 3294690 N/A 3294692 unspecified role unspecified role unspecified role unspecified role Region 28-42/259-660 Region 28-42/259-660 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false