DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ beck-2014-1 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. DIP original experiment: DIP-260624X Structure (London, England : 1993) 2014 IMEx CdiA from Enterobacter cloacae delivers a toxic ribosomal RNase into target bacteria. Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. Only protein-protein interactions imex curation beck-2014-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. DIP original experiment: DIP-260626X Structure (London, England : 1993) 2014 IMEx CdiA from Enterobacter cloacae delivers a toxic ribosomal RNase into target bacteria. Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. Only protein-protein interactions imex curation beck-2014-3 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. DIP original experiment: DIP-260627X Structure (London, England : 1993) 2014 IMEx CdiA from Enterobacter cloacae delivers a toxic ribosomal RNase into target bacteria. Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. Only protein-protein interactions imex curation beck-2014-4 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. DIP original experiment: DIP-260625X Structure (London, England : 1993) 2014 IMEx CdiA from Enterobacter cloacae delivers a toxic ribosomal RNase into target bacteria. Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. Only protein-protein interactions imex curation beck-2014-5 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. DIP original experiment: DIP-260628X Structure (London, England : 1993) 2014 IMEx CdiA from Enterobacter cloacae delivers a toxic ribosomal RNase into target bacteria. Beck CM., Morse RP., Cunningham DA., Iniguez A., Low DA., Goulding CW., Hayes CS. Only protein-protein interactions imex curation cdiiecl CdiI protein protein protein entcc Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56) MFGIFSKGEPVSMEGELVQPSSIVINDYEEEHPSYWIKYNSWLSLEGLNKTHSAASMYEPEKTNFFTLYFEDEKVYVQNVIFLEECHGFSPENINKFIERTTHGDGMKISEHTDLNSVLDFYHSLNN cdia_entcc 16S rRNA endonuclease CdiA cdiA Toxin CdiA ECL_04451 CdiA-ECL protein protein entcc Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56) MMKQDQVRFSQRALSALLSVLLATQPLLPAVAASITPSGNTQMDKAANGVPVVNIATPNQSGISHNKYNDYNVGKEGLILNNATGQLNQTQLGGLIQNNPNLKAGQEAKGIINEVTGANRSNLQGYTEVAGKAANVIVANPYGITCNGCGFINTPNVTLTTGKPVLDASGKLQSLDVTQGAVTIEGAGLNGSQSDAVSIISRATEINVQLHAKDLRVVAGANRVAADGSVSALKGEGTAPKVAVDTGALGGMYANRIRLVSSETGVGVNLGNLNARQGDIALSSAGKVVLKNTLASGSTTVSAADVTLRGDHKAGGNVTVSGQTALTLDQAHVAADNNLQLTTRGTLTQNGGAFTAANDATLAATTLIQSVDAQASAGRHLAVNAEKNAALNGSVVAGQQLSVKGGELVQQGNLSASEIALNAQTLTQESRSTTNASGNITLTTSGHSQLKGSTTAGQSLAVSAGSLANHGALAAVADTRINTGIFSNTGTVQGNSLTVSGTDITSSGALKSASTLDIRADNATLSGETGAKGKTTVTASGNLNNSGTLISDDTLTLNAAQIVNSGTLSGVRGLTTSGKTFTASATSVTQSDGDVALNNTDTTLAGETSAGGAVTVQGRSLNTTATAQTQGNSVGVAVQNAKLEGTQAAKGNMTLKADSSLNHTGKSSASGLKVETGHLSNSGTLTASALVIDSPEVINGGLIHAGQTLSLVTRLLDNRSSGVLYSPSALSLSLSELNNAGIITSDAALSLSGSNLTNSGELSGTSLAIDYETLKNSAEGMLLAQGANRITAQSVSSAGSMVGNTLTLNADRLESAGLLQGDSALSLTAGILNLLTGSRTLTGGALGLSGTTLTTAGQLQGQDVSIRSHDWTNRGSSLATGSLDVTTAGTLSNTGELMSQGNGTLNAVTTVNSGNMLSAGDLSLNGKTLRNSGTLQGNRVTAHQDTITNSGTLTGIAALMLAARLEMAAPLLTLVNDASGSLLTAGELSVTGGDLRNAGQWQGKRVLIHAQALTNGGAIQAENLLDAQIDSTLTGTAGSKITSNGELALSALTLANSGQWIAKHLTLGASTLNNSGEITGVVALSVALTQLNNQAGGKLLSAGALTLDVENATNAGQIQGKATTVTAGQLINSGRLQGEALTLNASGALNNTASGVLLSENALTVSTATLNNQGTLQGGGESSVKATTRVQNDGKMLSGGKLTLTAPELANSSSGLVQAVRLLLDVVKAVNGGNVLATTRAELRGSSLDNSGTLQGADLQANYQSVTNSGTVLGTTSLTINGDALDNTESGKLYSGDKLLLDVRNYSGRGDVVSLGDTTLKLVNALVNTGTLAASKTLSVSSQNAMTNSGVMQGNAIALSAGGAFTNNGTLTTGNGSSTFNAQSLLLNASGSLQAGGDVQLTSRENITVNGFTGTAGSLTMTAAGTLLNTALIYAGNNISLFAARIHNIYGDILADNSLWMQKNAVGEANAEVVNRSGTIETTRGDITVNTGHLLNEADGLTVSQSEREYPDAIPAADEHYFSYDLNGRRSDFVLLLEDWKNDGSKVVYDWYEQCLGSGANGSGQCRDRVDYRLTGEDIRQFLLSESVVSVSATGSSARIAAGRDITINAGTLDNRASHILAGRNAVLAGGTLNNLSAEGGRRVTYVQAEYRCEWFYRDCSDSKWEPLTQYPDGSWGWFDEDYGWYGWVPYILGERTTEFVADGGVYRSVISAGGNVSANFTSDISNTNVTANSGEFSNTIDAPTLNTLSPEAIGKGLNSESLAQGGSADIRFPEQLGNITDALKDISGGSSLSDQNGSSGNYPLPSGNNGYFVPSTDPDSPYLITVNPKLDELGNMDDSLFNGLYDLLGITPGATPRETNSAYTDRNQFLGSSYFLDRLGLNPDRDYRFLGDAAFDTRYVSNAILNQTGSRYINGIGSDLDQMRYLMDSAAEQQKTLGLKFGVALTAEQVAALDKSMLWWESATINGQTVMIPKVYLSPKDVTVHSGSVISGNNVQLAGGNVINSGSTIAAQNGLSIDSSNSLSNLNAGLLSAGGGLNLSALGDINNIGSTISGKTVGLESVAGSINNITRAQQWNVDAGNVHFSGTDVGKTASITATDGLTMRAGQDINVTGANVSAGGSLGMAAGNDINITANEIVTSEGRAGRNRATTETASVTHQGSTLSAGDDLTLQAGNDVNARAAAIAAEGDVGIQAGRDVDLLAEASMERSSSQAKKKTAIDESVRQQGTEIASGGNTVILAGRDVTAQAADVTAQGDIGVAAGRDVNLTTATESDYRYREQTKTSSGFLSKKTTHTIEEESATREKGSLLSGDNVTVSAGNNLRVLGSAVAGDGDVALSAGNNVDIVAATNTDTAWRFKETKKSGLMGTGGIGFTIGSSKSTHDLREQGTTQSESFSTVGSTGGNVSIAAGKQAHIGGADIIAQKDISLTGDSVVIEPGHDKRTRDEKFEQKSSGLTVALSGAAGSAVNNAVTTAQSAKQSSDSRLAALQGTQAALSGVQAGQAVALDQVKGDSDKRNNNTIGVSASIGSQSSKSSSHMESETTTGSTLSAGNNVTIKATGSDITVAGSQIKAGKDVTLDAARDVNLIASQDTQQTTGKNSSSGGSLGVGVGVGSGGAGISISANANSSKGHEKGNGVWQNETTVDAGNRVTINTGRDATIAGAQVSGETVVADIGRDLTIASTQDSDHYNSKQNSVSGGAGYTFGAGGFSGSINVSRDKMTSDYDSVQEQSGLFAGNGGFDVTVGNHTQLDSGVIASTATADKNRLDTGTLGFSDIHNQADFKTEHQGAGISSGGSIGKQFAGNMANALLAGGGNSGHAEGTTQAAVSEGTLIIRDKENQKQDVADLSRDAEHANGSISPIFDKEKEQQRLQEVQLIGEIGSQVVDIANTQGEINGLNAGRKELADKGITEPGADASDEVKAAYQNALRETDAYKTTTAKYGTGSDLQRGIQAATAALQGLAGSDLTAALAGASAPELAYRIGHGMGIDNNTAAKTIAHAILGGAVAALQGNSAAAGAAGAATGELAAKAIAGMLYPDVKDLSTLSEEQKQTVSALATISAGMAGGLAGDSTGSAVAGGQAGKNAAENNSLALVARGCAVAAPCRTKVAEQLLEIGAKAGIAGLAGAAVKDMADKMTSDELEHLVTLEMMGNDEIIAKYVSLLHDKYAPSHTGGNLLPETLPGHTGNNTGSVDTGPNHTGNTNRQNDSGSNNTGNTEGAPNTGGNTTITPIPNGPSKDDIAYLALKGKEAQEAASNLGFDRRIPPQKAPFNSHGQPVFYDGKNYITPDIDSHNVTNGWKMFNSKGKRIGTYDSGLNRIKD p94772_dicch Toxin CdiA hecA protein protein erwch Erwinia chrysanthemi Pectobacterium chrysanthemi Erwinia chrysanthemi MLSRYWAFHTAKLRSSGMKAVKTSQRVMVWALVWLTGLQPVLPAWAAGVTVASGNTALEAAGNGVPVVNIATPDASGLSHNRYHDFNVDNRGLILNNGTARLTPSQLGGLIQNNPNLNGRAAAAILNEVVSPNRSRLAGYLEVAGQAANVVVANPYGITCSGCGFLNTPRLTLTTGTPQFDAAGGLSGLDVRGGDILIDGAGLDASRSDYFGLIARTASLQAGLNARDAQVVLGANRVGADGRVTAQAGSGPAPVLALDTGALGGMYANRIRLVSTEQGVGVNTAGLSAREGDIRLSANGRLQVRGAVAQGELTAQGETLALQGNQQAQGSITLRGAQGVTLTGSRTRAGQGLTLASDGRITAADAGLSAGVREDGTVQPGDGLSLTGRELALGQSQLAGDRVSLNSTGAVSQSRAGVAGGSVLTVNGGALSLDGDAGAQTLTVSGSGLSGSGRWQATGDLTLDGLDRGAVGRGAAGGRGAVGAGGEPGQPGHAGGRAGTLTTPGLDNRGTVSGRQVAVRTAQLGNAGTLSADETLTIQAQTGLDNSGSLLSGGALTIAAGQTDNRGVLSGGAVTLSGDSLWNGGTLQGRQSLGVNALAGFSQTADGALTSGGTVTVSSGTLETAGALSAQGLQLRTGLWRNQGAVSLTGDGQLTVDELDNSGTLLSSGAWDIAGGRLGNSGTLQGDRLTLRGDRLDNQGALTGTTQTALRLGGALANRGTVSGNRLAVTAAALDNGGTLLGVEALTLTTDGALTNRGTGRLLTQGAAVLTAASVVNAGEGQAGRLQLTGGTLANTGTLAVNGGASLTLDGLDNRGTLSAGGDLTVTGADLRNAGQMAAQGALTLTGNYGGAGSLYSEGALSLSGAALVNGGGRWQGATVAVRGGPLTNSGTVTGLTALTVTTDGTLSNTGRLEGRRLDLTAEALDNGGTLLGVDALTLAITGTARNQAGGRFLSQGDGRLTAATLDNQGDWQGGRIDVTAGRVRNAGQVLGIAALTLTADNALTNTGTGRLLTPGAAVLTAATAVNDGEWQAGSLRLTADRLRNGGRIHSDGDLVVTLPTADGDPRRRAAQRLAQEVQTLGAGELSNSGTLVADGDGRLTARQVDNAGTLSTGGALTLTAGAITNAGRLESRTLSLTGDSLDNGGTLLAEQGGGLTLSDRLTVGADGQVLSNGDWQIQAGAVTSLGQWQGKNLRLSADTLTHDGVLQAERDITLALLQDYTGGAGSQVRGNGAVTLTADRVTQQGDIGGERLQLTTGTLTNGGRLVGLSQLDVTSRGQLTNSADGALLGNGTAGITAAALSNAGVLQGDALTVRAGTVDNAGSMQGTAALTLDGVTRYDGGADSRLLSGGAMTLALDTADNGGVWQAGELRVSGTSLTNRGQITGLSGLTIDATGLSNAGRLATQGRATLRGRQFDNGGTLTALGDLTADFRDGIVNQAGGQLLSGGAGQLTTGTLTNAGWVQGQDLTLTADTLFNQGSLLGLDDGAIQLTGAYVGGVNSRVGGNGAFSLSAATIDQAGQWQARDVTLRATRLRNQGTLTAGGQLTATLDDALENTAGAVLSGGTVSLGAATVSNAGQLEGRHGLTVAGGSRLDNQRGGQLLSGGQLALSAPQLTNAGWVQGQDLTLTTAQLDNGGTLQAQSGLTLHLPQWTNRGTVQAGQLDITTDGALENRGTLLGLTRLALQAARLDNADGARLYSAGNLQLRTGQLVQNGQLAALGDLRADIGNAFTFTRTLAAGGQLTLNVTGDLVQAGTLQGNGVTVTSTGTLTQQGRIVAGTGDSTLSAAAINQTASGSIQAGGALRLRAEGNIVNRGFVGTAADLLLQAGGVIDNGGLLYGGGNLWLLSDALVNRFGNILAGNSLWIQRDAAGNASGSVLNSSGTIETQRGDITVRTGTLTNQREGLVVTEGESKTEVVPDWVGGERVEVPLTWFKEGELGIAEFYTGCLRGGKASGANCEYSAGYLLAPFSSAAIQKVALESKSVSVSAQGGEARINSAHDTLITSSILTNEASAIYARNNIVLSGNSLNNTSYQAGDLKRYLTYRYDSVEFVYGTWSWINDFANDDQSAYVGGGSSPITKQLDLADKFEIQNKHYSINYKPVGEPTSELINGQTYAATIQAGGAITASFTQNISNTSLQPGSGGVMPALATPTLAGVSAFTPVGAQAGRELSGGTAAAVSGSPLSGTGNGVALAGQAERPGTAAGAVTRAGTDAGGGTLTPAGIDSGLGTAAPVAPGALSPGDLQAALRQGLAQVAGPSLTDYPLPTSQNGLFVADTAGDSRYLIRSNPTLSQLGQVDNSLFGDLRGLLGQTPGTSVPVETTPTLTDPTQFLGSSYLLGKLNLDAEHDYRFLGDAAFDTRYISNAVLSQTGQRYLNGVGSELAQMQQLMDNAAAEKSRLNLQLGVSLSPAQVAGLSHSIVWWENITVGGQTVLAPKLYLAQADNTHLQGSRLVADRVSLSAGGDIDNRGSTVTAQEVLNIASGGNLSNSEGGLLSAGGALNLVALGNLTNRSATLQGNTVTLASVNGDIVNSTTTDQWQFESINGRERLTHTDIGQTGLITAQNGLTLQAGHDIVLNGAQLSAGGPLALAAGNDIQLNALTTLTDTVREGGGATTERRSQGLVRSTVAGGGDLSLSAGRDLSGTAAQLSAAGTLALSAGRDLSLLSAREEQFGSNAWSRHLDWQQTVTQQGTGLNAGEGLSLRAGQDLTLQGAQAETRGALTAQAGRDLSLLSATESRHDFFEETTVKKKTFSTTVTHTVRETAQTTEKGTLLSAGSVALTAGQDIGVQGSSVAADGGVALTAGRDITTAASVENYRSYEEQSRKKSGLFSGGGIGFTVGSTSLRQTLESAGTTQSQSVSTLGSTGGSVSLRAGQDVSLTGTDVIAARDIDLSGRNVTVTPGHDVRRTTQTLEQKQSGLTIALSGSVGGALNSMVETVQAVSRESDSRLKSLAGVKAALSAGQGAQATRLAMAQREAAGAKTAAGSGEEGEQPQAVGVSISYGSQSSRSEQRQTQETVSGSSVTAGDNLRIRATDGDITVVGSQLKAGQDLTLAATQDIRLLSGANTQHTEGSNQSRGGSIGVSIGVSASGSFGLSVSASVNAAKGNLRGDGLTHTESLLEAGRTAVLGSGRDTTLQGAQVSAETITARVGRDLLVRSEQDSDRYDSKQQSVSAGVTIPIYGGGGGASFSFSRDKVHSNFDSVQEQSGLFAGTGGYDIHVGSHTQLDGGAIASTAGADRSRLETGTLGFSNIDNRAEYSASHTGGGFSTSAPVGLQVLSNVGGLMLAGANQSGASSGTTYAAVSDGTLIIRDRAGQQQVRGGAEPGYGGGQQRGAEPDIRQGEGGEPAAAGTTAVGHRDAGAGYRLYGRGDSGDEGGECAAGGDAGGSAAGEGGRAGEGLAGQGGNAGGGDAGAVPGVLQCVAERLGVSDGGTGTSGYPGGGGGAAGCAGGECGAGGDGGGGAVCGGGNPPSDDGCGGQHERDGEHAGARVAGCGGGAGGRETMRWRCGGEAGGELAARALMEGLYPGKKADELNEDQRQLLSTLSTIAGGLAAGVVGNSSTDAVQGAQSAQVAVENNLLSAKRSQDRYEKLAACNGDKACVAEVRREFGPESDEQRQRVENCSSAADCYVVEQGLKSMRAEYSQQEAALAEKARTQGVSSLSEAEQKEWIAARSALTELDSQINLSLHRAQTMGGSTEVSAEVTNVMGHAAIASAAGVAGGISKAGANGSKNQGGNTDKLPNGQQVNHFEESLYNLPPGERVALVKQMVDQVAPSNGMVKDNKLTRINNRDVYRGNDGYLYAVDTQHGRFEQVNAKTGKHQGEVDMGMMPISNSMDKSGGHDLKVK q84ba0_dicch Imm45 domain-containing protein protein protein erwch Erwinia chrysanthemi Pectobacterium chrysanthemi Erwinia chrysanthemi MQEPVRLIEIKNIALRHGNIFRLPAVWPYEALVDFMVVDLSNADRPYGLIVSSGHKAGLILVKLPAECCLSEVRGLSVEWVIDNWEKWIYPECDVKDVYVIDRYEILDSQ cdiiecl-cdia-1 3263802 N/A 3263807 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3263808 unspecified role unspecified role prey prey Region 3087-3321 Region 3087-3321 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false cdia-cdiiecl-1 3263803 N/A 3263807 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3263808 unspecified role unspecified role unspecified role unspecified role Region 3087-3321 Region 3087-3321 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdiiecl-cdia-2 3263804 N/A 3263807 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3263808 unspecified role unspecified role prey prey Region 3087-3321 Region 3087-3321 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdia-cdiiecl-2 3263805 N/A 3263807 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3263808 unspecified role unspecified role unspecified role unspecified role Region 3087-3321 Region 3087-3321 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false q84ba0_dicch-heca-1 3263806 N/A 3263809 unspecified role unspecified role prey prey Region 3576-3848 Region 3576-3848 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3263810 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false