DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ peisley-2014-1 ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel tag fluorescence tag visualisation by fluorescence tag fluorescence Peisley A., Wu B., Xu H., Chen ZJ., Hur S. DIP original experiment: DIP-260832X Nature 2014 IMEx Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Peisley A., Wu B., Xu H., Chen ZJ., Hur S. Only protein-protein interactions imex curation peisley-2014-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Peisley A., Wu B., Xu H., Chen ZJ., Hur S. DIP original experiment: DIP-260833X Nature 2014 IMEx Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Peisley A., Wu B., Xu H., Chen ZJ., Hur S. Only protein-protein interactions imex curation o95786-1 Antiviral innate immune response receptor RIG-I Probable ATP-dependent RNA helicase DDX58 DDX58 DEAD box protein 58 RIG-I-like receptor 1 Retinoic acid-inducible gene 1 protein Retinoic acid-inducible gene I protein protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ddx58-7 3263407 N/A 3263409 unspecified role unspecified role unspecified role unspecified role Region 1-200 Region 1-200 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O95786-1:p.[Arg17Ala;Lys18Ala] R17A/K18A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O95786-1:p.Asp21Ala D21A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O95786-1:p.[Glu35Ala;Glu36Ala] E35A/E36A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O95786-1:p.[Lys108Ala;Arg109Ala] K108A/R109A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O95786-1:p.[Glu137Ala;Glu138Ala] E137A/E138A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O95786-1:p.Glu153Ala E153A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O95786-1:p.[Asp163Ala;Lys164Ala] D163A/K164A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Alexa647-label Alexa647-label alexa label alexa label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false ddx58-ub-3 3263408 N/A 3263410 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3263409 unspecified role unspecified role unspecified role unspecified role Region 1-200 Region 1-200 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false