DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kast-2014-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260852X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-2 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260859X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260854X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-4 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260855X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-5 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260857X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260853X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-7 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260861X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-8 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260858X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-9 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260856X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation kast-2014-10 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. DIP original experiment: DIP-260860X Nature structural & molecular biology 2014 IMEx Mechanism of IRSp53 inhibition and combinatorial activation by Cdc42 and downstream effectors. Kast DJ., Yang C., Disanza A., Boczkowska M., Madasu Y., Scita G., Svitkina T., Dominguez R. Only protein-protein interactions imex curation q9uqb8-4 Brain-specific angiogenesis inhibitor 1-associated protein 2 BAIAP2-alpha BAIAP2 Fas ligand-associated factor 3 Insulin receptor substrate p53/p58 Insulin receptor substrate protein of 53 kDa protein protein human Homo sapiens Human MSLSRSEEMHRLTENVYKTIMEQFNPSLRNFIAMGKNYEKALAGVTYAAKGYFDALVKMGELASESQGSKELGDVLFQMAEVHRQIQNQLEEMLKSFHNELLTQLEQKVELDSRYLSAALKKYQTEQRSKGDALDKCQAELKKLRKKSQGSKNPQKYSDKELQYIDAISNKQGELENYVSDGYKTALTEERRRFCFLVEKQCAVAKNSAAYHSKGKELLAQKLPLWQQACADPSKIPERAVQLMQQVASNGATLPSALSASKSNLVISDPIPGAKPLPVPPELAPFVGRMSAQESTPIMNGVTGPDGEDYSPWADRKAAQPKSLSPPQSQSKLSDSYSNTLPVRKSVTPKNSYATTENKTLPRSSSMAAGLERNGRMRVKAIFSHAAGDNSTLLSFKEGDLITLLVPEARDGWHYGESEKTKMRGWFPFSYTRVLDSDGSDRLHMSLQQGKSSSTGNLLDKDDLAIPPPDYGAASRAFPAQTASGFKQRPYSVAVPAFSQGLDDYGARSMSSGSGTLVSTV p60953-2 Cell division control protein 42 homolog CDC42 G25K GTP-binding protein Placental protein protein human Homo sapiens Human MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL eps8_human Epidermal growth factor receptor kinase substrate 8 EPS8 protein protein human Homo sapiens Human MNGHISNHPSSFGMYPSQMNGYGSSPTFSQTDREHGSKTSAKALYEQRKNYARDSVSSVSDISQYRVEHLTTFVLDRKDAMITVDDGIRKLKLLDAKGKVWTQDMILQVDDRAVSLIDLESKNELENFPLNTIQHCQAVMHSCSYDSVLALVCKEPTQNKPDLHLFQCDEVKANLISEDIESAISDSKGGKQKRRPDALRMISNADPSIPPPPRAPAPAPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHEQEETPEMMAARIDRDVQILNHILDDIEFFITKLQKAAEAFSELSKRKKNKKGKRKGPGEGVLTLRAKPPPPDEFLDCFQKFKHGFNLLAKLKSHIQNPSAADLVHFLFTPLNMVVQATGGPELASSVLSPLLNKDTIDFLNYTVNGDERQLWMSLGGTWMKARAEWPKEQFIPPYVPRFRNGWEPPMLNFMGATMEQDLYQLAESVANVAEHQRKQEIKRLSTEHSSVSEYHPADGYAFSSNIYTRGSHLDQGEAAVAFKPTSNRHIDRNYEPLKTQPKKYAKSKYDFVARNNSELSVLKDDILEILDDRKQWWKVRNASGDSGFVPNNILDIVRPPESGLGRADPPYTHTIQKQRMEYGPRPADTPPAPSPPPTPAPVPVPLPPSTPAPVPVSKVPANITRQNSSSSDSGGSIVRDSQRHKQLPVDRRKSQMEEVQDELIHRLTIGRSAAQKKFHVPRQNVPVINITYDSTPEDVKTWLQSKGFNPVTVNSLGVLNGAQLFSLNKDELRTVCPEGARVYSQITVQKAALEDSSGSSELQEIMRRRQEKISAAASDSGVESFDEGSSH baiap2-cdc42-4 3263325 N/A 3263335 unspecified role unspecified role unspecified role unspecified role Region 260-291 Region 260-291 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3263336 unspecified role unspecified role unspecified role unspecified role Region 1-178 Region 1-178 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P60953-2:p.Gly12Val G12V mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-eps8-4 3263326 N/A 3263337 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3263335 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false baiap2-cdc42-5 3263327 N/A 3263335 unspecified role unspecified role unspecified role unspecified role Region 1-452 Region 1-452 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.[Pro278Asp;Pro281Asp] P278D/P281D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.[Ile267Ala;Ser268Ala] I267A/S268A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.[Leu277Glu;Phe286Glu] L277E/F286E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.Phe286Glu F286E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3263336 unspecified role unspecified role unspecified role unspecified role Region 1-178 Region 1-178 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P60953-2:p.Gly12Val G12V mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-cdc42-6 3263328 N/A 3263335 unspecified role unspecified role fret pair fluorescence acceptor donor pair fret pair Region 1-452 Region 1-452 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide certain certain sequence position certain certain certain sequence position certain false Rhodamine-label Rhodamine-label rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain false Q9UQB8-4:p.[Pro278Asp;Pro281Asp] P278D/P281D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3263336 unspecified role unspecified role neutral component neutral component Region 1-178 Region 1-178 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P60953-2:p.Gly12Val G12V mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-eps8-5 3263329 N/A 3263337 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli N/A 3263335 unspecified role unspecified role fret pair fluorescence acceptor donor pair fret pair Region 1-452 Region 1-452 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide certain certain sequence position certain certain certain sequence position certain false Rhodamine-label Rhodamine-label rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-cdc42-7 3263330 N/A 3263335 unspecified role unspecified role unspecified role unspecified role Region 260-291 Region 260-291 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3263336 unspecified role unspecified role unspecified role unspecified role Region 1-178 Region 1-178 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P60953-2:p.Gly12Val G12V mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-1 3263331 N/A 3263335 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false baiap2-eps8-6 3263332 N/A 3263337 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3263335 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false baiap2-cdc42-8 3263333 N/A 3263335 unspecified role unspecified role fret pair fluorescence acceptor donor pair fret pair Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide certain certain sequence position certain certain certain sequence position certain false Rhodamine-label Rhodamine-label rhodamine label rhodamine label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3263336 unspecified role unspecified role neutral component neutral component Region 1-178 Region 1-178 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P60953-2:p.Gly12Val G12V mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false baiap2-5 3263334 N/A 3263335 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false