DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mechaly-2014-1 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mechaly AE., Sassoon N., Betton JM., Alzari PM. DIP original experiment: DIP-259773X PLoS biology 2014 IMEx Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Mechaly AE., Sassoon N., Betton JM., Alzari PM. Only protein-protein interactions imex curation mechaly-2014-2 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Mechaly AE., Sassoon N., Betton JM., Alzari PM. DIP original experiment: DIP-259770X PLoS biology 2014 IMEx Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Mechaly AE., Sassoon N., Betton JM., Alzari PM. Only protein-protein interactions imex curation mechaly-2014-3 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Mechaly AE., Sassoon N., Betton JM., Alzari PM. DIP original experiment: DIP-259771X PLoS biology 2014 IMEx Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Mechaly AE., Sassoon N., Betton JM., Alzari PM. Only protein-protein interactions imex curation mechaly-2014-4 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mechaly AE., Sassoon N., Betton JM., Alzari PM. DIP original experiment: DIP-259772X PLoS biology 2014 IMEx Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Mechaly AE., Sassoon N., Betton JM., Alzari PM. Only protein-protein interactions imex curation cpxr_ecoli Transcriptional regulatory protein CpxR cpxR b3912 JW3883 yiiA protein protein ecoli Escherichia coli (strain K12) MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDSIDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPFNDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGTEFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWFKTLRGRGYLMVSAS cpxa_ecoli Sensor histidine kinase CpxA ecfB eup ssd JW3882 b3911 cpxA protein protein ecoli Escherichia coli (strain K12) MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQRQGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEMQIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLLFDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEFLAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELERIETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTVNFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVSPEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIWLPLYKRS cpxa-cpxr-3 3262688 N/A 3262692 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-115 Region 1-115 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3262693 enzyme enzyme unspecified role unspecified role P0AE82:p.Met228Val M228V mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false cpxa-1 3262689 N/A 3262693 unspecified role unspecified role unspecified role unspecified role Region 188-457 Region 188-457 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cpxa-2 3262690 N/A 3262693 unspecified role unspecified role unspecified role unspecified role Region 188-457 Region 188-457 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cpxa-3 3262691 N/A 3262693 putative self putative self unspecified role unspecified role Region 188-457 Region 188-457 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0AE82:p.Met228Val M228V mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false