DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kim-2014-8 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260145X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation kim-2014-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260142X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation kim-2014-10 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260148X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation kim-2014-11 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260146X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation kim-2014-12 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260150X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation kim-2014-13 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260143X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation kim-2014-14 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260144X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation kim-2014-15 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260147X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation kim-2014-16 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. DIP original experiment: DIP-260149X Nature chemical biology 2014 IMEx Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. Kim DG., Lee JY., Kwon NH., Fang P., Zhang Q., Wang J., Young NL., Guo M., Cho HY., Mushtaq AU., Jeon YH., Choi JW., Han JM., Kang HW., Joo JE., Hur Y., Kang W., Yang H., Nam DH., Lee MS., Lee JW., Kim ES., Moon A., Kim K., Kim D., Kang EJ., Moon Y., Rhee KH., Han BW., Yang JS., Han G., Yang WS., Lee C., Wang MW., Kim S. Only protein-protein interactions imex curation syk_human Lysine--tRNA ligase KARS1 KARS KIAA0070 Lysyl-tRNA synthetase protein protein human Homo sapiens Human MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV rssa_human 40S ribosomal protein SA RPSA LAMBR LAMR1 Laminin receptor 1 37/67 kDa laminin receptor Laminin-binding protein precursor p40 Colon carcinoma laminin-binding protein NEM/1CHD4 Multidrug resistance-associated protein MGr1-Ag 37 kDa laminin receptor precursor 67 kDa laminin receptor Small ribosomal subunit protein uS2 protein protein human Homo sapiens Human MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS aimp2_human Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 Protein JTV-1 AIMP2 PRO0992 JTV1 Multisynthase complex auxiliary component p38 protein protein human Homo sapiens Human MPMYQVKPYHGGGAPLRVELPTCMYRLPNVHGRSYGPAPGAGHVQEESNLSLQALESRQDDILKRLYELKAAVDGLSKMIQTPDADLDVTNIIQADEPTTLTTNALDLNSVLGKDYGALKDIVINANPASPPLSLLVLHRLLCEHFRVLSTVHTHSSVKSVPENLLKCFGEQNKKQPRQDYQLGFTLIWKNVPKTQMKFSIQTMCPIEGEGNIARFLFSLFGQKHNAVNATLIDSWVDIAIFQLKEGSSKEKAAVFRSMNSALGKSPWLAGNELTVADVVLWSVLQQIGGCSVTVPANVQRWMRSCENLAPFNTALKLLK rssa_mouse 40S ribosomal protein SA Rpsa Lamr1 Laminin receptor 1 37/67 kDa laminin receptor Laminin-binding protein precursor p40 37 kDa oncofetal antigen OFA/iLRP P40-8 37 kDa laminin receptor precursor 67 kDa laminin receptor protein protein mouse Mus musculus Mouse MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTAAQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTEWS syk_mouse Lysine--tRNA ligase Kars1 Kars Lysyl-tRNA synthetase protein protein mouse Mus musculus Mouse MATLQESEVKVDGEQKLSKNELKRRLKAEKKLAEKEAKQKELSEKQLNQTASAPNHTADNGVGAEEETLDPNQYYKIRSQAVQQLKVTGEDPYPHKFHVDISLTQFIQEYSHLQPGDHLTDVTLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFVHINNKLRRGDIIGVEGNPGKTKKGELSIIPQEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIVRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVKSITGSYKITYHPDGPEGQAYEVDFTPPFRRISMVEELEKALGVKLPETSLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCISPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPEDKKETAATTETPESTEASPSV mca3_human Eukaryotic translation elongation factor 1 epsilon-1 Multisynthase complex auxiliary component p18 Elongation factor p18 EEF1E1 P18 AIMP3 Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 protein protein human Homo sapiens Human MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNSYLEDKVYLTGYNFTLADILLYYGLHRFIVDLTVQEKEKYLNVSRWFCHIQHYPGIRQHLSSVVFIKNRLYTNSH symc_human Methionine--tRNA ligase, cytoplasmic MARS1 MARS Methionyl-tRNA synthetase protein protein human Homo sapiens Human MRLFVSDGVPGCLPVLAAAGRARGRAEVLISTVGPEDCVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGSVRRALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLLQDPAYLPEELSALHSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPSPAEGRAVTNEPEEEELATLSEEEIAMAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKALEEGLTPQEICDKYHIIHADIYRWFNISFDIFGRTTTPQQTKITQDIFQQLLKRGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK kars-rpsa-6 3259989 N/A 3259998 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3259999 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false kars-rpsa-7 3259990 N/A 3259999 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3259998 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false kars-rpsa-8 3259991 N/A 3259998 unspecified role unspecified role bait bait Q15046:p.Leu142Ala L142A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q15046:p.Phe144Ala F144A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259999 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false kars-aimp2-2 3259992 N/A 3260000 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3259998 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false aimp2-kars-10 3259993 N/A 3260000 unspecified role unspecified role unspecified role unspecified role Region 1-48 Region 1-48 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259998 unspecified role unspecified role unspecified role unspecified role Q15046:p.Ser207Asp S207D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q15046:p.Thr52Asp T52D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false kars-6 3259994 N/A 3259998 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false kars-rpsa-9 3259995 N/A 3260001 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3260002 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false mars-eef1e1-1 3259996 N/A 3260003 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3260004 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false kars-rpsa-10 3259997 N/A 3259998 unspecified role unspecified role bait bait Q15046:p.Leu142Ala L142A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q15046:p.Phe144Ala F144A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3259999 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false