DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lukhele-2013-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Lukhele S., Bah A., Lin H., Sonenberg N., Forman-Kay JD. DIP original experiment: DIP-255881X Structure (London, England : 1993) 2013 IMEx Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface. Lukhele S., Bah A., Lin H., Sonenberg N., Forman-Kay JD. Only protein-protein interactions imex curation lukhele-2013-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lukhele S., Bah A., Lin H., Sonenberg N., Forman-Kay JD. DIP original experiment: DIP-255882X Structure (London, England : 1993) 2013 IMEx Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface. Lukhele S., Bah A., Lin H., Sonenberg N., Forman-Kay JD. Only protein-protein interactions imex curation if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV 4ebp2_human Eukaryotic translation initiation factor 4E-binding protein 2 EIF4EBP2 protein protein human Homo sapiens Human MSSSAGSGHQPSQSRAIPTRTVAISDAAQLPHDYCTTPGGTLFSTTPGGTRIIYDRKFLLDRRNSPMAQTPPCHLPNIPGVTSPGTLIEDSKVEVNNLNNLNNHDRKHAVGDDAQFEMDI eif4e-eif4ebp2-7 3259926 N/A 3259928 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3259929 unspecified role unspecified role unspecified role unspecified role Q13542:p.[Tyr54Ala;Leu59Ala;Ile78Ala;Pro79Ala;Gly80Ala;Val81Ala;Thr82Ala] Y54A/L59A/I78A/P79A/G80A/V81A/T82A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false eif4e-eif4ebp2-8 3259927 N/A 3259928 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3259929 unspecified role unspecified role unspecified role unspecified role Q13542:p.[Ile78Ala;Pro79Ala;Gly80Ala;Val81Ala;Thr82Ala] I78A/P79A/G80A/V81A/T82A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13542:p.[Tyr54Ala;Leu59Ala] Y54A/L59A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false Deletion of region 78-82 decreased the interaction rate/strength.