DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ke-2013-1 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. DIP original experiment: DIP-255686X Nature structural & molecular biology 2013 IMEx Structural basis for RNA recognition by a dimeric PPR-protein complex. Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. Only protein-protein interactions imex curation ke-2013-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. DIP original experiment: DIP-255683X Nature structural & molecular biology 2013 IMEx Structural basis for RNA recognition by a dimeric PPR-protein complex. Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. Only protein-protein interactions imex curation ke-2013-3 in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. DIP original experiment: DIP-255684X Nature structural & molecular biology 2013 IMEx Structural basis for RNA recognition by a dimeric PPR-protein complex. Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. Only protein-protein interactions imex curation ke-2013-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. DIP original experiment: DIP-255687X Nature structural & molecular biology 2013 IMEx Structural basis for RNA recognition by a dimeric PPR-protein complex. Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. Only protein-protein interactions imex curation ke-2013-5 in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. DIP original experiment: DIP-255688X Nature structural & molecular biology 2013 IMEx Structural basis for RNA recognition by a dimeric PPR-protein complex. Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. Only protein-protein interactions imex curation ke-2013-6 in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. DIP original experiment: DIP-255685X Nature structural & molecular biology 2013 IMEx Structural basis for RNA recognition by a dimeric PPR-protein complex. Ke J., Chen RZ., Ban T., Zhou XE., Gu X., Tan MH., Chen C., Kang Y., Brunzelle JS., Zhu JK., Melcher K., Xu HE. Only protein-protein interactions imex curation i1hb13_bradi 100823749 BRADI_2g00245v3 BRADI_2g00270v3 protein protein bradi Brachypodium distachyon Purple false brome Trachynia distachya MASLLFLPHTTPAPTLRAKLLPGPRTIITCGPRDNRGPLQRGRSLSTEAIHAVQALKRLTAADRSPPAATAAASAALGRLLRADLLAAMAELQRQGHWSLALAALHVARAEPWYRPDPELYATFVSSSPSNDPAAAAAVDALVEAFIEEKERGAAGGSSEGVWVGEDVYKLTRLVRALVAKGRARAAWRVYEAAVRKGGCEVDEYMYRVMAKGMKRLGLDEEAAEVEADLADWEARHLPDEMRPREKSKTAVTGSVV tha8l_arath Protein THYLAKOID ASSEMBLY 8-like, chloroplastic THA8L T6H20.100 At3g46870 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTAIRVCSRKFPTFASIFFQNITRNPSIHRISFSNLKPKTLLHPIPPKPFTVFVSRFHDGRPRGPLWRGKKLIGKEALFVILGLKRLKEDDEKLDKFIKTHVFRLLKLDMLAVIGELERQEETALAIKMFEVIQKQEWYQPDVFMYKDLIVSLAKSKRMDEAMALWEKMKKENLFPDSQTYTEVIRGFLRDGCPADAMNVYEDMLKSPDPPEELPFRVLLKGLLPHPLLRNKVKKDFEELFPEKHAYDPPEEIFGRC i1hb13_bradi-i1hb13_bradi-1 3259806 N/A 3259812 unspecified role unspecified role unspecified role unspecified role Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3259812 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false i1hb13_bradi-1 3259807 N/A 3259812 unspecified role unspecified role unspecified role unspecified role I1HB13:p.Ser99Arg S99R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false I1HB13:p.Tyr169Ser Y169S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false i1hb13_bradi-2 3259808 N/A 3259812 unspecified role unspecified role unspecified role unspecified role I1HB13:p.Ser99Arg S99R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false I1HB13:p.Tyr169Ser Y169S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false i1hb13_bradi-3 3259809 N/A 3259812 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pp266_arath-pp266_arath-1 3259810 N/A 3259813 unspecified role unspecified role unspecified role unspecified role Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3259813 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false i1hb13_bradi-4 3259811 N/A 3259812 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false