DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dang-2013-1 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. DIP original experiment: DIP-255892X Nature 2013 IMEx Inhibitory signalling to the Arp2/3 complex steers cell migration. Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. Only protein-protein interactions imex curation dang-2013-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. DIP original experiment: DIP-255893X Nature 2013 IMEx Inhibitory signalling to the Arp2/3 complex steers cell migration. Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. Only protein-protein interactions imex curation dang-2013-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. DIP original experiment: DIP-255895X Nature 2013 IMEx Inhibitory signalling to the Arp2/3 complex steers cell migration. Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. Only protein-protein interactions imex curation dang-2013-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. DIP original experiment: DIP-255894X Nature 2013 IMEx Inhibitory signalling to the Arp2/3 complex steers cell migration. Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. Only protein-protein interactions imex curation dang-2013-5 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. DIP original experiment: DIP-255896X Nature 2013 IMEx Inhibitory signalling to the Arp2/3 complex steers cell migration. Dang I., Gorelik R., Sousa-Blin C., Derivery E., Guérin C., Linkner J., Nemethova M., Dumortier JG., Giger FA., Chipysheva TA., Ermilova VD., Vacher S., Campanacci V., Herrada I., Planson AG., Fetics S., Henriot V., David V., Oguievetskaia K., Lakisic G., Pierre F., Steffen A., Boyreau A., Peyriéras N., Rottner K., Zinn-Justin S., Cherfils J., Bièche I., Alexandrova AY., David NB., Small JV., Faix J., Blanchoin L., Gautreau A. Only protein-protein interactions imex curation arpin_mouse Arpin Arpin Arp2/3 inhibition protein protein protein mouse Mus musculus Mouse MSRIYQDSALRNKAVQSARLPGTWDPATHQGGNGILLEGELVDVSRHSILDAHGRKERYYVLYIQPSCIHRRKFDPKGNEIEPNFSATRKVNTGFLMSSYKVEAKGDTDRLTLEALKSLVNKPQLLELTESLTPDQAVAFWMPESEMEVMELELGTGVRLKTRGDGPFIDSLAKLELGTVTKCNFAGDGKTGASWTDNIMAQKSSERNTAEIREQGDGAEDEEWDD mint arpc5_mouse Actin-related protein 2/3 complex subunit 5 Arpc5 Arp2/3 complex 16 kDa subunit protein protein mouse Mus musculus Mouse MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRQGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAVLLQWHEKALAAGGVGSIVRVLTARKTV arpc2_human Actin-related protein 2/3 complex subunit 2 ARPC2 ARC34 PRO2446 Arp2/3 complex 34 kDa subunit protein protein human Homo sapiens Human MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR q7z6k5-1 Arpin ARPIN C15orf38 Arp2/3 inhibition protein protein protein human Homo sapiens Human MSRIYHDGALRNKAVQSVRLPGAWDPAAHQGGNGVLLEGELIDVSRHSILDTHGRKERYYVLYIRPSHIHRRKFDAKGNEIEPNFSATRKVNTGFLMSSYKVEAKGDTDRLTPEALKGLVNKPELLALTESLTPDHTVAFWMPESEMEVMELELGAGVRLKTRGDGPFLDSLAKLEAGTVTKCNFTGDGKTGASWTDNIMAQKCSKGAAAEIREQGDGAEDEEWDD wasl_human Neural Wiskott-Aldrich syndrome protein WASL protein protein human Homo sapiens Human MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED p63000-1 Ras-related C3 botulinum toxin substrate 1 RAC1 Cell migration-inducing gene 5 protein Ras-like protein TC25 TC25 p21-Rac1 MIG5 Rac1A protein protein human Homo sapiens Human MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL mint wasf2_human Wiskott-Aldrich syndrome protein family member 2 WASF2 WAVE2 Protein WAVE-2 Verprolin homology domain-containing protein 2 protein protein human Homo sapiens Human MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD arpin-arpc5-1 3259522 N/A 3259527 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3259528 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false arpin-arpc2-1 3259523 N/A 3259529 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3259530 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human physical association physical association false false false wasl-arpc2-1 3259524 N/A 3259531 unspecified role unspecified role bait bait Region 392-505 Region 392-505 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3259529 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false arpin-arpc2-2 3259525 N/A 3259529 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3259530 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 211-226 Region 211-226 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false rac1-wasf2-1 3259526 N/A 3259532 unspecified role unspecified role bait bait P63000-1:p.Gln61Leu Q61L mutation increasing strength mutation increasing interaction strength mutation increasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3259533 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false