DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sun-2013-5 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266808X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-6 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266799X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-7 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266803X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-8 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266806X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-9 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266802X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-10 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266809X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-11 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266801X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-12 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266810X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-13 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266807X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-14 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266800X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-15 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266804X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation sun-2013-16 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266805X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions imex curation bak1_arath BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 BAK1 SERK3 At4g33430 F17M5.190 Somatic embryogenesis receptor-like kinase 3 ELG Somatic embryogenesis receptor kinase 3 Protein ELONGATED protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERRLMIPCFFWLILVLDLVLRVSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCTWFHVTCNSDNSVTRVDLGNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNNSLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSLFTPISFANTKLTPLPASPPPPISPTPPSPAGSNRITGAIAGGVAAGAALLFAVPAIALAWWRRKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIGDSTSQIENEYPSGPR flica_pseai A-type flagellin fliC flaA protein protein pseai Pseudomonas aeruginosa MALTVNTNIASLNTQRNLNNSSASLNTSLQRLSTGSRINSAKDDAAGLQIANRLTSQVNGLNVATKNANDGISLAQTAEGALQQSTNILQRMRDLSLQSANGSNSDSERTALNGEVKQLQKELDRISNTTTFGGRKLLDGSFGVASFQVGSAANEIISVGIDEMSAESLNGTYFKADGGGAVTAATASGTVDIAIGITGGSAVNVKVDMKGNETAEQAAAKIAAAVNDANVGIGAFSDGDTISYVSKAGKDGSGAITSAVSGVVIADTGSTGVGTAAGVTPSATAFAKTNDTVAKIDISTAKGAQSAVLVIDEAIKQIDAQRADLGAVQNRFDNTINNLKNIGENVSAARGRIEDTDFAAETANLTKNQVLQQAGTAILAQANQLPQSVLSLLR fls2_arath LRR receptor-like serine/threonine-protein kinase FLS2 FLS2 Protein FLAGELLIN-SENSING 2 Protein FLAGELLIN-SENSITIVE 2 MPL12.13 MPL12.8 At5g46330 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLLSKTFLILTLTFFFFGIALAKQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKRTRVILIILGSAAALLLVLLLVLILTCCKKKEKKIENSSESSLPDLDSALKLKRFEPKELEQATDSFNSANIIGSSSLSTVYKGQLEDGTVIAVKVLNLKEFSAESDKWFYTEAKTLSQLKHRNLVKILGFAWESGKTKALVLPFMENGNLEDTIHGSAAPIGSLLEKIDLCVHIASGIDYLHSGYGFPIVHCDLKPANILLDSDRVAHVSDFGTARILGFREDGSTTASTSAFEGTIGYLAPEFAYMRKVTTKADVFSFGIIMMELMTKQRPTSLNDEDSQDMTLRQLVEKSIGNGRKGMVRVLDMELGDSIVSLKQEEAIEDFLKLCLFCTSSRPEDRPDMNEILTHLMKLRGKANSFREDRNEDREV flic-bak1-1 3259132 N/A 3259144 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3259145 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P21184:p.Gly47Tyr G47Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P21184:p.Gly47Ala G47A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false bak1-flic-1 3259133 N/A 3259145 unspecified role unspecified role unspecified role unspecified role Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3259144 unspecified role unspecified role unspecified role unspecified role Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3259146 unspecified role unspecified role unspecified role unspecified role Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false flic-bak1-2 3259134 N/A 3259144 unspecified role unspecified role prey prey Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q94F62:p.Leu53Ala L53A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q94F62:p.Val54Tyr V54Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q94F62:p.Tyr96Ala Y96A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q94F62:p.[Phe60Ala;Phe144Ala] F60A/F144A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3259145 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false bak1-fls2-4 3259135 N/A 3259144 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-249 Region 1-249 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3259146 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false flic-fls2-1 3259136 N/A 3259145 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259146 unspecified role unspecified role prey prey Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false flic-fls2-2 3259137 N/A 3259145 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P21184:p.Asp43Ala D43A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P21184:p.Asp44Ala D44A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P21184:p.Ala46Tyr A46Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259146 unspecified role unspecified role prey prey Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false flic-bak1-3 3259138 N/A 3259144 unspecified role unspecified role prey prey Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3259145 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259146 unspecified role unspecified role prey prey Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper association association false false false fls2-bak1-3 3259139 N/A 3259144 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3259146 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9FL28:p.[Arg294Ala;His316Ala] R294A/H316A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9FL28:p.Thr342Tyr T342Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9FL28:p.Ser437Tyr S437Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9FL28:p.Asp414Ala D414A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false fls2-bak1-4 3259140 N/A 3259144 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3259146 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false flic-fls2-3 3259141 N/A 3259145 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259146 unspecified role unspecified role prey prey Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9FL28:p.[Arg294Ala;His316Ala] R294A/H316A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9FL28:p.Thr342Tyr T342Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9FL28:p.Ser437Tyr S437Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9FL28:p.Asp414Ala D414A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false fls2-bak1-5 3259142 N/A 3259144 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q94F62:p.Leu53Ala L53A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q94F62:p.Val54Tyr V54Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q94F62:p.[Phe60Ala;Phe144Ala] F60A/F144A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q94F62:p.Tyr96Ala Y96A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3259146 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false bak1-flic-2 3259143 N/A 3259145 unspecified role unspecified role unspecified role unspecified role Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3259144 unspecified role unspecified role unspecified role unspecified role Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3259146 unspecified role unspecified role unspecified role unspecified role Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false