DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ whitfield-2013-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. DIP original experiment: DIP-259707X PLoS biology 2013 IMEx A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony. Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. Only protein-protein interactions imex curation whitfield-2013-2 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. DIP original experiment: DIP-259703X PLoS biology 2013 IMEx A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony. Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. Only protein-protein interactions imex curation whitfield-2013-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. DIP original experiment: DIP-259704X PLoS biology 2013 IMEx A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony. Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. Only protein-protein interactions imex curation whitfield-2013-4 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. DIP original experiment: DIP-259706X PLoS biology 2013 IMEx A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony. Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. Only protein-protein interactions imex curation whitfield-2013-5 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. DIP original experiment: DIP-259705X PLoS biology 2013 IMEx A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony. Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. Only protein-protein interactions imex curation whitfield-2013-6 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. DIP original experiment: DIP-259708X PLoS biology 2013 IMEx A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony. Whitfield ZJ., Chisholm J., Hawley RS., Orr-Weaver TL. Only protein-protein interactions imex curation ccna_drome G2/mitotic-specific cyclin-A CycA CG5940 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASFQIHQDMSNKENPGIKIPAGVKNTKQPLAVIGGKAEKNALAPRANFAVLNGNNNVPRPAGKVQVFRDVRNLNVDENVEYGAKKSNVVPVVEQFKTFSVYEDNNDTQVAPSGKSLASLVDKENHDVKFGAGQKELVDYDLDSTPMSVTDVQSPMSVDRSILGVIQSSDISVGTETGVSPTGRVKELPPRNDRQRFLEVVQYQMDILEYFRESEKKHRPKPLYMRRQKDISHNMRSILIDWLVEVSEEYKLDTETLYLSVFYLDRFLSQMAVVRSKLQLVGTAAMYIAAKYEEIYPPEVGEFVFLTDDSYTKAQVLRMEQVILKILSFDLCTPTAYVFINTYAVLCDMPEKLKYMTLYISELSLMEGETYLQYLPSLMSSASVALARHILGMEMWTPRLEEITTYKLEDLKTVVLHLCHTHKTAKELNTQAMREKYNRDTYKKVAMMESVEMSKDDFDQLCEAYNCKQKEDEHQQPDINTKSNVNLFYKF cort_drome Protein cortex cort CG11330 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFVEMSVCDSPNKNSKLDKKSLTPFKKVRRKNWKQEAAYKSDTSKGQEVSYVGERFIPNRFERENIEFNLKYIGKRKERDILETGVTLTASYWRQSGFISNINRTFGIGERRLFQFSSQQGTRSRVVDNDSADSDWPCNPRARPYAIQNATHEMPGICSPVDYNMMDWSSGGMVAMSSGQDVMLWRNLDESTMVFSVESPTSLKYSPDGKHLAIGCMDRNYPVLDLWEVRSPTEFLVSYRKLFFKSMGYISCIEWSHDGKEVICGTQCGVIIVLAMPTLNTLMQLREHRHTVKKMKFAPTHKYFASSDTDGKIFIFDAVLKVRLLKLDGRSIVFDWHPWTGEDLAVAERSPASIFIFNIPRRQFVASYRRRDDRIVIKTLTYSKITGELLVNVIRRDDADLAVCEILVLASLNRVVDLMSHQDRGTLFLMWNPDGTKIATGGLDDTFSLWNFFPTYKREAILRKQEQKAKDKCSSLSLYKGIR q9vas9_drome Apc CG1451 APC APC1 Apc1 D-APC D-APC1 DAPC Dm APC1 Dmel\CG1451 apc apc 1 apc1 d-APC dAPC dAPC1 dApc dapc1 Dmel_CG1451 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLEPTLTPDLEEGIAELSLEDVDVDSVPERKPHFLDYDAVPPPDYEEGFGASSGEQLKMDKKYERDGEVSDYELAASGYTKKEFTQDDNTLHFTQSAVGLGSGAVGKKPAAKHFLDENPIPPDYMLAQELREMREHRSLDRNFERQSAQQQQLDELPPRNGGGSPASAGRPSRSKEPSYTLSRFLDGDAPAPAPRLPKGAAWTTSFDERYTSSAVEATLGSKVECVYSLLSMLGSNDPLEMAKKFLELSGNAQSCATLRRSGCMPLLVQMMHAPDNDQEVRKCAGQALHNVVHSHPDEKAGRREAKVLRLLDQIVDYCSFLKTLLQSGGEAIADDSDRHPLAAISSLMKVSFDEEHRHAMCELGALHAIPNLVHLDHAVHGPKPEDQCCNSLRRYALMALTNLTFGDENNKALLCGQKQFMEALVAQLDSAPDDLLQVTASVLRNLSWRADSNMKAVLNEIGTVTALALAAMRNRSENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLKIIENAGGILRNVSSHIAVCEPYRQILRQHNCLAILLQQLKSESLTVVSNSCGTLWNLSARSAEDQKFLWDNGAVPMLRSLIHSKHAMISEGSSSALKNLLNFRPAVQNHHQLDPIARSMGLKALPTLEARKAKALQQELGERHTAETCDNLDTGGKLDKERASSSSRRHPAPRLTRSAMLTKSESRDSVYSAKSDCAYDHLIRSASASDAHRKVKPKITDFDLEMEQDTEATEEQPIDYSVKYSENATKTSTYQETDLDQPTDFSLRYAENQIESDLDISGPAGGQKSTITPPAETVPEKSEGQEILLILDDSVKCYQTEDTPYVISNAASVTDLRVAAKADAEAEVKPEVREVTSKEGAPKKLPKLSQCGSGSYTPEKPINYCEEGTPGYFSRYDSLSSLDESGKANQAIVGTDADIKPKLEKQEEQESQPAEQVLTKPPTQANSALETPLMFSRRSSMDSLVHDPDVDVANCDDKSSVVSDFSRLASGVISPSEIPDSPTQSMPQSPRRNSVAGSGQNVDSPPVVIPASLQPLRSVFEDDLSSFNVEHTPAQFSTATSLSNLSIVDDEKAPASVAEEDNEDELLLANCINMGMQRKPTEAVKSTVVNSEVDVAEETIRSYCTEDTPALLSKVPSNTNLSVISMSSTDPKDATAGQAQMYAHQLSDDVSSNASDCGGASGHLLQQCIRDGMKKPLGEATSDPIAMLRRGGNELPGYLPSADEMNKFLVEDSPCNFSVVSGLSNLTVGSSLVGPAVQLKETEPSSADQNPEMKAKPGKQEQVRRPPHWQDDSLSSLSIDSEDDTNLLSQAIAAGCNRPKSNLGFSSNGKRSSSLSSSQPIAINAATSASSLNSAMTVRKSQQQESYSSVDSSDSNDNQSKSLFELCILKGMYKTKEPGARAQQMQEQPIVGSSSVQSNPSLKQFDSLPVQLPSSGQVKRQRHHHHHHHHRERERERKDEKLLQECINTGISKKINAVPKNVLATSAAALEPCHPMAATTSASALSTAAPDVEQKAHATSNPQQQSSTHPSSHILPNPIDAIATVTDTVRSPAAPNQGNGNASQNGLETATGSKDLDSEDRSSDESNQSFIMETMVRLDSALNETCISGASEKHKDPDLMLKSVERLTMEFVTSAEQLRSSSHNHSSSNSHKNNSSNNTWNESTCPNDVSFPSVSQTAPVLASLSLDEDATEARSLHELIEITPTNEQQPESLEGETDTLVNGHADSYSGSSGGLNFQLGGQVQNAGVRLEPQRLLFNGTSASIMTNSTMIAFEARALAENLLQPAATDDDTTEMTFSLNSLDLDNIRPPSGMESLNSCYQDHSQPSSLRQAMPSKSPRFARKMFPANLVARRALGHLAGSAESVNSSCNLLDNIKPPSLMDELLDSMISVDSIQSEVADGEQDCSMATTISVSNYETAACDDQTMTVLQSCFDEDEDATMNDYSSAESTPKHGSTPSPNRRSLTPKDKRRLTKDRFKTYTIATSCEMEAPEANETLQIEIVEAAVPVATPSPRANGRRRGSAERYKTQLIECPLALIQPQPDDCPSEQLSSIRAMMQQFTFITDINIGHSQETCESTDHPEDAGESPECDQNSETESCDGQEPDQLPPPPSIVDLRTSVVKPTTLEPATAVKLVRGRKKPAYVSPYSMQSQRNSNNAAPSKKKTLSPTIAKRSLVPGGSGVRLPAKKKPTPPPEPAPARLERQGTFVKDEPTNSNVQVPVVETKPAQTSPTHRASKLPTKKGTASGGSPSKAGSPKRIPLAPARRMTPQRANTSLRLAAGKSHAASRVVSGRVSSTTPPSRSNSNLNGSSAAAAAAAKINHAQSRIANIWKRVDEAKTKQSSSNLRTQKTKSSNMLNANGTKPTLLRSSTFDNTPSTAGGVKSKLPVVGARK q9i7l8_drome CG2508 Cdc23 cdc23 Dmel_CG2508 38C.54 APC8 Apc8 CDC23 CG2508-PA Dmel\CG2508 APC8 (cdc23) protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAMQKFFSVLLPDVKRELRRGIIECSKRGLLHSTKWLAEMHHGLADVHIDNEAPDEDRTFSECQLEGIAPEEYSDYFLAKSYYDVREYDRAAHAVRNCESSVPRFLHFYSSYMAREKRRLDSTTDQANLHEPNQMRDLADLLATLRMEYGKSRLDGYGIYLYGVVLKALNLNQAAEQMLVQAIRLVPMLWSAYLELSPLIMEKKKLLSLQLGGHWMRHFFMAHTYLELYLNDDGLKIYEDLQASGFSKSIYLIAQMALVYHNKRDVDKAIELYQALLESDPYRLDNVDTYSNLLFVKEMKTEMAQLAHKAVSINKYRPETCCVIGNYYSIRCDHQVAISYFQRALKLNPKYLAAWTLMGHEFMELKNTNAAIQSYRKAVEVNKRDYRAWYGLGQAYEIIKMHYYSLYYFKIAHQLRPYDSRMLVALGETYEKLDKCENAVKCYWKAIDVGDIEGIAMYKLANLHEKLGDHETAVHCYIMYCEDERAATDKQSLYQGFITLANYYEKKGEYERAAYYAYKCLDSEDRKMEAKALLKTIDWKRNAEGQKKVKTSTAVANADTSSEDEMEWELQDVRVRVPITSIATTTTSTTTVAAESASSSSLISLRPDRNLIQGMRRSQSSRTSLTAPASGTTTTPSATAVTMSTEETPGTSGGPSNPPEQAPSDDNSSMEISSVSID q9vs37_drome Cdc27 APC3 Apc3 CDC27 CDC27Dm Dmcdc27 Dmel\CG8610 cdc27 l(3)L7123 mks Dmel_CG8610 CG8610 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMIQEPVQAAIWHCLNYYDFKDAVFLSERLCSEVESDETIFLLATSYFRSNQVHQAYWLLKEKARRSPQCRFLQAKCAYELKKYAEAESALISTGFADAKNCDELQRDFGDLACFAYQLMAQICMRTERNKLAVSALRRALKLNPFMWHAFADLCLLGQDTDAAAIFQIHSTDVFNTCQGSSVNANAMVLFGAEQQGQQHQERQSLITNLSNVSNYILTTPVDQQQQQINQNQNQHHNSNMVTPINNNNNNNNLNSSISMLRGGLVQNSSMLAMLEDTPMGAPQDPAGQYQQNQQLYDMSSGTPFRKQFKYLSAISPPTPSFGIMPLTSPCTGNDGSFIGNHTPVMNISYSPMPQMLVEVNQEPKMMGKKLKTHVGGLINRKEGSLNKPAVFTQAGNITPRTPNNNNVGNNGNMNVPPNPNAAVRRSSRLFSNSYSVKENNKSPNIANKFVQPRSPPRKAKSRMTKICLNNELIEDKSHHLSEKRKEKVETITSSGANNNSGGRSAAEEAKVLLNNSLNNAQTMAHQLMGLKKQSADGLMALLRGLAEAYQLLSNFQCKAAIKQLETTIPKHHLNSSWVQSLIGLARYEMREYEAAVAIFETIHKTEPCRLDYMEIYSSSLWHLQREVELSALAQDLINQDKTSPVTWCVSGNCFSLQKEHETAIKFFKRAVQVDPDFVYSYTLLGHELVLTEEFDKAMDYFRAAVVRDPRHYNAWYGIGTIYSKQEKYELAEIHYVKALKINPQNSVILVHIGAMQFYMKKKDLSLQTLNTAATLDPKNPLTRFHRGSIYFSLGKYQEALRELEELKEVVPKESVVFYLIGKIHKTLGNMDLALMHFSWATDLDPKGANNQIKDAFDSMAHPSCCPANTTALDVDLEPTSERSDDSTQAQQDGSYDSDY q9vcn6_drome CDC16 Dmcdc16 Dmel\CG6759 CG6759 APC6 Cdc16 cdc16 Dmel_CG6759 Apc6 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPGDTENTSTDTTNEHIDLALYRQLVKQFIDMRRYSTALFWAEKVAVLGGLEPRDIYYQAQCMYLLGEYHRAAHTIQHHKLEKNSLPCFNLLLESLYAAKEFTEAANVIQNVEVEVMTTSLINQPVDASSGCYLESNSVFGGEENHRNELLSSIYLMKGKVYEALDNRGMAMDFYVQALHKSIYCFEALEALVQHEMLMAWEEFELMHHLPLAQQSSEADAKFILKLYESRLKKYYELISARNAEEMSPIVNPDILQFIKEFTARVQQSGSSDTQMPKVSAVKVPLTPSQFMSPAQKVLEDLKAPTFSLQTSLSKASSLIDASHRSMFDSSSRRRSRDHDTDTLIPIAECLDRVQRSTDLMAAEAEKCFYDCDYKQCLKILNELLKVDPFHNTALTIQIACLVENGDFNRLFYVAHKLVDRYPDKAISWYAVGCYYDMIGKSDPARRYLSKATALDRLYGPAWLAYGHSFANENEHEQAMAAYFKATQLMRGCHLPLLYIGVECGLTKNLELAEKFFLQAMNIAPLDVYVLHELGVIKYEYEFFDGAATIFQCTVDIVKQRAKSNNEEISSRWEPLFINLGHSLRKVHKYEEALYNFQYALLLKPQSPTTYTSIGFIHALLGNLDPAIEAFHKSLALNRDCIVTSTILKSCIEDLMDDSATIDEICSAALRDVAKNITANSRRVLNSDKFNGMKLKFDEEEEFANSDSNMVVEMSFDT mtrm_drome Protein matrimony mtrm anon-D52 CG18543 Cell cycle arrest protein D52 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MENSRTPTNKTKITLNRTPTLKERRWNTLKVNTSNVRCSTPIFGNFRSPNLSPIENMGTKGKSPVSPMRFATFKKVPTKVHPKQQQQQQHQHCHRTQLKPPPFVLPKPQEEIIEPEREIKSCSSPDTCSDDSNMETSLALESRRRSIKASNHSYVVNHAANVEQILMHMGLENYVTNFEEAHIDLVELASLERADLVKIGLNTDEDCNRIMDVLHTL q24044_drome fzy-RA fzy Dmel_CG4274 BG:DS02740.14 CDC20 Cdc20 Cdc20[Fzy] Cdc20[fizzy] Dmel\CG4274 FZY Fizzy Fzy cdc20 dCDC20 dFZY fizzy CG4274 Cdc20FZY cdc2 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSQFNFVSDLQNALIMDGETRGPAPRWKKKLEASLNGSVNTTRSVLSVSYNTSFSGVQAPTKTPGKSSEGKTKKSNTTPSKTPGGGDRFIPNRAATNFELAHFLVNKDSGDKSDEENDKATSSNSNESNVQASAHKGDRQKLISEVAQVGDSKGGRILCYQNKAPAAPETHNNPLKVVYSIKTPISTKSGSRYIPTTSERILDAPDFINDYYLNLMDWSADNIVAVALGSCVYLWNAQTGNIEQLTEFEEGDYAGSLSWIQEGQILAIGNSTGAVELWDCSKVKRLRVMDGHSARVGSLAWNSFLVSSGSRDGTIVHHDVRAREHKLSTLSGHTQEVCGLKWSTDFKYLASGGNDNLVNVWSAASGGVGTATDPLHKFNDHQAAVRALAWCPWQPSTLASGGGTADRCIKFWNVNNGTLMKSVDSKSQVCSLLFSRHYKELISAHGFANNQLTIWKYPTMVKQADLTGHTSRVLQMAMSPDGSTVISAGADETLRLWNCFAPDPLASKKAVSTSKGKQSVFRQSIR cyca-cort-1 3258198 N/A 3258204 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3258205 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro direct interaction direct interaction false false false cort-apc-1 3258199 N/A 3258206 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258207 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258208 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258209 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258210 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258205 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly association association false false false mtrm-cort-1 3258200 N/A 3258210 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3258205 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro direct interaction direct interaction false false false Deletion of region 149-483 decreased the interaction rate/strength. cort-apc-2 3258201 N/A 3258206 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258207 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258209 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258210 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258205 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly association association false false false cort-apc-3 3258202 N/A 3258206 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258207 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258208 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258209 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258205 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly association association false false false cyca-fzy-1 3258203 N/A 3258211 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro N/A 3258204 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false