DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ni-2013-1 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255819X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255805X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-3 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255813X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-4 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255816X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-5 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255810X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-6 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255806X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-7 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255814X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-8 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255821X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-9 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255807X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-10 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255822X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-11 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255811X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-12 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255808X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-13 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255817X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-14 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255812X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-15 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255809X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-16 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255818X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-17 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255815X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation ni-2013-18 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. DIP original experiment: DIP-255820X Structure (London, England : 1993) 2013 IMEx Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Ni L., Li S., Yu J., Min J., Brautigam CA., Tomchick DR., Pan D., Luo X. Only protein-protein interactions imex curation stk3_human Serine/threonine-protein kinase 3 STE20-like kinase MST2 Mammalian STE20-like protein kinase 2 Serine/threonine-protein kinase Krs-1 STK3 MST2 KRS1 protein protein human Homo sapiens Human MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF q8www0-1 Ras association domain-containing protein 5 A NORE1A RASSF5 NORE1 RAPL New ras effector 1 Regulator for cell adhesion and polarization enriched in lymphoid tissues protein protein human Homo sapiens Human MAMASPAIGQRPYPLLLDPEPPRYLQSLSGPELPPPPPDRSSRLCVPAPLSTAPGAREGRSARRAARGNLEPPPRASRPARPLRPGLQQRLRRRPGAPRPRDVRSIFEQPQDPRVPAERGEGHCFAELVLPGGPGWCDLCGREVLRQALRCTNCKFTCHPECRSLIQLDCSQQEGLSRDRPSPESTLTVTFSQNVCKPVEETQRPPTLQEIKQKIDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLAGPDTEVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIQQVQKKYDKFRQKLEEALRESQGKPG mob1a_human MOB kinase activator 1A MOB1A C2orf6 MOB4B MOBK1B MOBKL1B Mob1 alpha Mob1 homolog 1B Mps one binder kinase activator-like 1B protein protein human Homo sapiens Human MSFLFSSRSSKTFKPKKNIPEGSHQYELLKHAEATLGSGNLRQAVMLPEGEDLNEWIAVNTVDFFNQINMLYGTITEFCTEASCPVMSAGPRYEYHWADGTNIKKPIKCSAPKYIDYLMTWVQDQLDDETLFPSKIGVPFPKNFMSVAKTILKRLFRVYAHIYHQHFDSVMQLQEEAHLNTSFKHFIFFVQEFNLIDRRELAPLQELIEKLGSKDR stk3-stk3-1 3257306 N/A 3257324 unspecified role unspecified role bait bait 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu452Ala L452A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257324 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu452Ala L452A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false stk3-1 3257307 N/A 3257324 unspecified role unspecified role unspecified role unspecified role Region 9-491 Region 9-491 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false rassf5-stk3-1 3257308 N/A 3257325 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3257324 unspecified role unspecified role unspecified role unspecified role pT pT opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false stk3-stk3-2 3257309 N/A 3257324 unspecified role unspecified role bait bait 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Ile463Ala I463A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257324 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Ile463Ala I463A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false mob1a-stk3-1 3257310 N/A 3257326 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3257324 enzyme enzyme unspecified role unspecified role Kinase domain Region 16-313 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q13188:p.Thr180Ala T180A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q13188:p.Asp146Asn D146N mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q13188:p.[Thr174Ala;Thr180Ala] T180A/T174A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13188:p.[Met227Ala;Ile230Ala] M227A/I230A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13188:p.His225Ala H225A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q13188:p.Phe231Ala F231A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false stk3-4 3257311 N/A 3257324 unspecified role unspecified role unspecified role unspecified role Kinase domain Region 16-313 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false stk3-stk3-3 3257312 N/A 3257324 unspecified role unspecified role bait bait 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu455Ala L455A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257324 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu455Ala L455A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false stk3-5 3257313 N/A 3257324 putative self putative self unspecified role unspecified role Q13188:p.Ile193Ala I193A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false pT pT opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false rassf5-stk3-2 3257314 N/A 3257325 unspecified role unspecified role unspecified role unspecified role Region 366-413 Region 366-413 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3257324 unspecified role unspecified role unspecified role unspecified role Region 9-313/428-491 Region 9-313/428-491 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false stk3-stk3-4 3257315 N/A 3257324 enzyme target enzyme target substrate substrate unspecified role unspecified role Kinase domain Region 16-313 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q13188:p.Asp146Asn D146N mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3257324 enzyme enzyme unspecified role unspecified role Q13188:p.Ile193Ala I193A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q13188:p.Phe231Ala F231A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Kinase domain Region 16-313 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pT pT opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false Q13188:p.Thr180Ala T180A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false rassf5-stk3-3 3257316 N/A 3257325 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3257324 unspecified role unspecified role prey prey Q13188:p.Lys473Ala K473A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false rassf5-stk3-4 3257317 N/A 3257325 unspecified role unspecified role unspecified role unspecified role Region 366-413 Region 366-413 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3257324 unspecified role unspecified role unspecified role unspecified role Region 9-313/428-491 Region 9-313/428-491 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false stk3-stk3-5 3257318 N/A 3257324 unspecified role unspecified role bait bait 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Tyr470Ala Y470A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257324 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Tyr470Ala Y470A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false stk3-stk3-6 3257319 N/A 3257324 unspecified role unspecified role bait bait 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu448Ala L448A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257324 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu448Ala L448A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false rassf5-stk3-5 3257320 N/A 3257325 unspecified role unspecified role bait bait Region 366-413 Region 366-413 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3257324 unspecified role unspecified role prey prey Region 9-313/428-491 Region 9-313/428-491 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false stk3-stk3-7 3257321 N/A 3257324 unspecified role unspecified role bait bait 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu478Ala L478A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257324 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu478Ala L478A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false stk3-stk3-8 3257322 N/A 3257324 unspecified role unspecified role bait bait 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Glu462Ala E462A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257324 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Glu462Ala E462A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false stk3-stk3-9 3257323 N/A 3257324 unspecified role unspecified role bait bait 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu445Ala L445A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257324 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q13188:p.Leu445Ala L445A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false