DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ li-2013-57 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254732X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Li Z., Hartl FU., Bracher A. Only protein-protein interactions imex curation li-2013-58 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254736X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Li Z., Hartl FU., Bracher A. Only protein-protein interactions imex curation li-2013-59 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254733X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Li Z., Hartl FU., Bracher A. Only protein-protein interactions imex curation li-2013-60 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254734X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Li Z., Hartl FU., Bracher A. Only protein-protein interactions imex curation li-2013-61 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254735X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Li Z., Hartl FU., Bracher A. Only protein-protein interactions imex curation f10a1_rat Hsc70-interacting protein Protein ST13 homolog Protein FAM10A1 St13 Fam10a1 Hip protein protein rat Rattus norvegicus (Rat) Rat MDPRKVSELRAFVKMCRQDPSVLHTEEMRFLREWVESMGGKVPPATHKAKSEENTKEEKRDKTTEDNIKTEEPSSEESDLEIDNEGVIEADTDAPQEMGDENAEITEAMMDEANEKKGAAIDALNDGELQKAIDLFTDAIKLNPRLAILYAKRASVFVKLQKPNAAIRDCDRAIEINPDSAQPYKWRGKAHRLLGHWEEAARDLALACKLDYDEDASAMLREVQPRAQKIAEHRRKYERKREEREIKERIERVKKAREEHEKAQREEEARRQSGSQFGSFPGGFPGGMPGNFPGGMPGMGGAMPGMAGMPGLNEILSDPEVLAAMQDPEVMVAFQDVAQNPSNMSKYQNNPKVMNLISKLSAKFGGHS hspa1a_hspa1b_human-1 HSPA1A_HSPA1B_human molecule set molecule set human Homo sapiens Human P0DMV8,P0DMV9 st13-1 3295233 N/A 3295238 unspecified role unspecified role unspecified role unspecified role Region 1-44 Region 1-44 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hsp70-st13-1 3295234 N/A 3295239 unspecified role unspecified role unspecified role unspecified role Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3295238 unspecified role unspecified role unspecified role unspecified role Region 78-247 Region 78-247 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P50503:p.Asn177Lys N177K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P50503:p.[Asp211Lys;Tyr212Ala] D211K/Y212A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false st13-2 3295235 N/A 3295238 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hsp70-st13-2 3295236 N/A 3295239 unspecified role unspecified role unspecified role unspecified role Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3295238 unspecified role unspecified role unspecified role unspecified role Region 78-247 Region 78-247 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P50503:p.Asn177Lys N177K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P50503:p.[Asn177Ala;Asp179Ala] N177A/D179A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P50503:p.Leu210Ser L210S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P50503:p.[Asp211Lys;Tyr212Ala] D211K/Y212A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P50503:p.Lys117Ala K117A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P50503:p.[Arg235Ala;Lys236Asp] R235A/K236D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P50503:p.Ser180Glu S180E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P50503:p.Ile148Ala I148A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false Deletion of region 235-247 decreased the interaction rate/strength. hsp70-st13-3 3295237 N/A 3295239 unspecified role unspecified role unspecified role unspecified role Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false T204A T204A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3295238 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false