DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhang-2013-24 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252322X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions imex curation zhang-2013-25 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252319X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions imex curation zhang-2013-26 ecolx Escherichia coli atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252316X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions imex curation zhang-2013-27 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252320X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions imex curation zhang-2013-28 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252317X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions imex curation zhang-2013-29 ecolx Escherichia coli 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252318X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions imex curation zhang-2013-30 ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252321X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions imex curation huth_rat Histidine ammonia-lyase Hal Huth protein protein rat Rattus norvegicus (Rat) Rat MPRYTVHVRGEWLAVPCQDGKLSVGWLGREAVRRYMKNKPDNGGFTSVDEVRFLVRRCKGLGLLDNEDLLEVALEDNEFVEVVIEGDVMSPDFIPSQPEGVFLYSKYREPEKYIALDGDSLSTEDLVNLGKGHYKIKLTSIAEKKVQQSREVIDSIIKERTVVYGITTGFGKFARTVIPANKLQELQVNLVRSHSSGVGKPLSPERCRMLLALRINVLAKGYSGISLETLKQVIEVFNASCLSYVPEKGTVGASGDLAPLSHLALGLIGEGKMWSPKSGWADAKYVLEAHGLKPIVLKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDTDIHAVRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKDIITTELNSATDNPMVFASRGETISGGNFHGEYPAKALDYLAIGVHELAAISERRIERLCNPSLSELPAFLVAEGGLNSGFMIAHCTAAALVSESKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLDQKVWEVAAPYIEKYRMEHIPESRPLSPTAFSLESLRKNSATIPESDDL pard3_rat Partitioning defective 3 homolog Pard3 Par3 Atypical PKC isotype-specific-interacting protein Atypical PKC-specific-binding protein protein protein rat Rattus norvegicus (Rat) Rat MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFRPYQTTSEIEVTPSVLRANMPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTTGSPKTCDRKKDENYRSLPRDPSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRARVIWFHVVPAANKEQYEQLSQREMNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPTAPALAPPNVLSTSVGSVYNTKRVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEPSQMQSPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGESGKCQLSPTVNMPHDDTVMIEDDRLPVLPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSNASQLDFVKTRKSKSMDLGIADETKLNTVDDQRAGSPNRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNGDPEKRDKAEKKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSEEDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYDGCLALNARPQSPREGHLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQSGRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSVSQDSWEQNYAPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS hal-1 3253847 N/A 3253854 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-89 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pard3-1 3253848 N/A 3253855 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pard3-3 3253849 N/A 3253855 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pard3-4 3253850 N/A 3253855 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9Z340:p.Arg9Ala R9A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9Z340:p.Thr4Glu T4E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9Z340:p.[Val13Asp;Asp70Lys] V13D/D70K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pard3-5 3253851 N/A 3253855 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pard3-6 3253852 N/A 3253855 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pard3-7 3253853 N/A 3253855 unspecified role unspecified role unspecified role unspecified role Q9Z340:p.[Val13Asp;Asp70Lys] V13D/D70K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false