DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2013-23 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Wang T., Fu G., Pan X., Wu J., Gong X., Wang J., Shi Y. DIP original experiment: DIP-252864X Nature 2013 IMEx Structure of a bacterial energy-coupling factor transporter. Wang T., Fu G., Pan X., Wu J., Gong X., Wang J., Shi Y. Only protein-protein interactions imex curation wang-2013-24 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang T., Fu G., Pan X., Wu J., Gong X., Wang J., Shi Y. DIP original experiment: DIP-252866X Nature 2013 IMEx Structure of a bacterial energy-coupling factor transporter. Wang T., Fu G., Pan X., Wu J., Gong X., Wang J., Shi Y. Only protein-protein interactions imex curation wang-2013-25 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Wang T., Fu G., Pan X., Wu J., Gong X., Wang J., Shi Y. DIP original experiment: DIP-252865X Nature 2013 IMEx Structure of a bacterial energy-coupling factor transporter. Wang T., Fu G., Pan X., Wu J., Gong X., Wang J., Shi Y. Only protein-protein interactions imex curation q03nm0_levba LVIS_2151 protein protein lacba Lactobacillus brevis (strain ATCC 367 / JCM 1170) MTKGSLKENTIAAVLIAMTVALSILVVIPIPATKGIVTLCEVGIYTSAILYGRRMGLLVGGASGFLIDILTGYPVWCLFSLVIHGTQGLVVGWLLPRHHKGIRSMLLPLLVGSLVMVIGYCLATTLLFGWPAGLASIFGNVVQVGFGAGVTLSIVGPLTRLKPDLV ecfa1_levba Energy-coupling factor transporter ATP-binding protein EcfA1 ecfA1 cbiO1 LVIS_1662 protein protein lacba Lactobacillus brevis (strain ATCC 367 / JCM 1170) MTENIISVDHLTYQYDENQAPALTDVSFTVHAGEWLAIVGHNGSGKSTLAKSLDGLLPFTQGSVTVGGITLTPETVWQVREQIGMIFQNPDNQFVGATVEDDVAFGLENRQISRDEMVPRVQAALAQVGMTSFAQREPSSLSGGQKQRVALAGIVAIAPKILILDEATSMLDPQGRIEMLAIVRQLRQQQNLTVISITHDIDEAASADRVLVIDDGRLVDEAVPSQIFERGTQLVEMGLDLPFTEKLKAALRQRGITPPTTYQTAAEMEEWLWQSLSNT ecfa2_levba Energy-coupling factor transporter ATP-binding protein EcfA2 ecfA2 cbiO2 LVIS_1661 protein protein lacba Lactobacillus brevis (strain ATCC 367 / JCM 1170) MAIAFEHVTYTYQAGTPMAHTALTDVSLTVPDRGYLAIIGHTGSGKSTLIQQLNALLKPTSGTIKIDEFTITPETTNAALKPLRQHVGMVFQFPENQLFEETVRQDIAFGPKNFGMADADALALADEMLTTVGLDQSYAERSPFELSGGQMRRVAIAGVLAMQPKVLVLDEPTAGLDPQGRQEMMRLFARLHQEQGLTIVLVTHQMEDVAQYAEQVAVMHEGRLMKFGTPADVFSNREWLQDHQLDVPQAAQFARRLRDRGLTFPKQPLTADQLADYLAQQWAQRGADHV ecft_levba Energy-coupling factor transporter transmembrane protein EcfT ecfT LVIS_1660 protein protein lacba Lactobacillus brevis (strain ATCC 367 / JCM 1170) MSNFIFGRYLPLDSVVHRLDPRAKLMLSFCYIIVVFLANNIWSYAILIAFTVGAILSSKISLGFFLKGIRPLLWLIVFTVVLQLLFSPAGGHTYFHWAFINVTQDGLINAGYIFVRFLLIIMMSTLLTLSTQPLDIATGLASLMKPLRWVKVPVDTLAMMLSIALRFVPTLMDEATKIMNAQRARGVDFGEGGLFKQAKSLIPLMVPLFMSAFNRAEDLSTAMEARGYQDSEHRSQYRILTWQRRDTVTWLLFLLGFVAILIFRHW ecft-ecfa1-1 3252903 N/A 3252906 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3252907 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3252908 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3252909 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false ecfa1-ecfa2-1 3252904 N/A 3252906 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252907 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252908 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252909 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false ecfa1-ecfa2-2 3252905 N/A 3252906 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252907 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252908 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252909 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false