DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chandra-2013-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chandra V., Huang P., Potluri N., Wu D., Kim Y., Rastinejad F. DIP original experiment: DIP-252825X Nature 2013 IMEx Multidomain integration in the structure of the HNF-4α nuclear receptor complex. Chandra V., Huang P., Potluri N., Wu D., Kim Y., Rastinejad F. Only protein-protein interactions imex curation chandra-2013-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chandra V., Huang P., Potluri N., Wu D., Kim Y., Rastinejad F. DIP original experiment: DIP-252826X Nature 2013 IMEx Multidomain integration in the structure of the HNF-4α nuclear receptor complex. Chandra V., Huang P., Potluri N., Wu D., Kim Y., Rastinejad F. Only protein-protein interactions imex curation p41235-2 Hepatocyte nuclear factor 4-alpha HNF4A HNF4 NR2A1 Nuclear receptor subfamily 2 group A member 1 TCF14 Transcription factor 14 Transcription factor HNF-4 HNF4-A protein protein human Homo sapiens Human MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMSTPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI Produced by alternative splicing of isoform HNF4-Alpha-1. coactivator peptide Coactivator peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EKHKILHRLLQDSY coa-hnf4a-1 3251828 N/A 3251830 unspecified role unspecified role unspecified role unspecified role Region 46-368 Region 46-368 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3251831 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction false false false coa-hnf4a-2 3251829 N/A 3251830 unspecified role unspecified role unspecified role unspecified role Region 46-368 Region 46-368 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3251831 unspecified role unspecified role unspecified role unspecified role in vitro In vitro physical association physical association false false false