IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ paster-2013-3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-1 in vitro In vitro peptide array peptide array tag visualisation tag visualisation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-4 human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-8 human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. Details of the Human GRB2 mutant plasmids gifted by Dr. R.A. Weinberg were obtained from Addgene. It has been assumed the WT construct is similar. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-7 in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-9 in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-2 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down anti tag western anti tag western blot anti tag western Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. Details of the Human GRB2 mutant plasmids gifted by Dr. R.A. Weinberg were obtained from Addgene. It has been assumed the WT construct is similar. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-5 human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. LAT-deficient Jurkat J.CaM2.5 cells were used. LAT was determined to be of Human origin and with identifier O43561-2 by mutation mapping. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-6 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-10 human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions paster-2013-12 human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Only protein-protein interactions zap70_human Tyrosine-protein kinase ZAP-70 ZAP70 SRK Syk-related tyrosine kinase 70 kDa zeta-chain associated protein protein protein human Homo sapiens Human MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA lck_human Tyrosine-protein kinase Lck Protein YT16 Proto-oncogene Lck T cell-specific protein-tyrosine kinase LCK Lymphocyte cell-specific protein-tyrosine kinase p56-LCK Leukocyte C-terminal Src kinase protein protein human Homo sapiens Human MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP q8n1k5-1 Protein THEMIS THEMIS C6orf190 C6orf207 Thymocyte-expressed molecule involved in selection protein protein human Homo sapiens Human MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV plcg1_human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 PLC-148 PLC1 Phospholipase C-II PLCG1 Phosphoinositide phospholipase C-gamma-1 Phospholipase C-gamma-1 protein protein human Homo sapiens Human MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL lat_human Linker for activation of T-cells family member 1 LAT 36 kDa phospho-tyrosine adapter protein p36-38 protein protein human Homo sapiens Human MEEAILVPCVLGLLLLPILAMLMALCVHCHRLPGSYDSTSSDSLYPRGIQFKRPHTVAPWPPAYPPVTSYPPLSQPDLLPIPRSPQPLGGSHRTPSSRRDSDGANSVASYENEGASGIRGAQAGWGVWGPSWTRLTPVSLPPEPACEDADEDEDDYHNPGYLVVLPDSTPATSTAAPSAPALSTPGIRDSAFSMESIDDYVNVPESGESAEASLDGSREYVNVSQELHPGAAKTEPAALSSQEAEEVEEEGAPDYENLQELN o43561-2 Linker for activation of T-cells family member 1 36 kDa phospho-tyrosine adapter protein p36-38 LAT Short protein protein human Homo sapiens Human MEEAILVPCVLGLLLLPILAMLMALCVHCHRLPGSYDSTSSDSLYPRGIQFKRPHTVAPWPPAYPPVTSYPPLSQPDLLPIPRSPQPLGGSHRTPSSRRDSDGANSVASYENEEPACEDADEDEDDYHNPGYLVVLPDSTPATSTAAPSAPALSTPGIRDSAFSMESIDDYVNVPESGESAEASLDGSREYVNVSQELHPGAAKTEPAALSSQEAEEVEEEGAPDYENLQELN mint thms1_human Protein THEMIS THEMIS C6orf190 C6orf207 Thymocyte-expressed molecule involved in selection protein protein human Homo sapiens Human MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK thms1_mouse Protein THEMIS Themis Grb2-associating protein Protein thylex Thymocyte-expressed molecule involved in selection protein protein mouse Mus musculus Mouse MALSLEEFVYSLDLRTLPRVLEIQSGIYFEGSVYEMFGNECCLSTGEVIKITGLKIKKMMAEICEGAIGGCESQKPFELPMNFPGLFKVMADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYLLISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQKSKHGPLQPQAPL themis-grb2-8 2971514 N/A 2971525 unspecified role unspecified role prey prey tyr493 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false N/A 2971526 unspecified role unspecified role prey prey N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false 4E themis-lck-1 2971514 N/A 2971525 unspecified role unspecified role prey prey N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 4Ei There was a strong ZAP70-pY493 signal only when Lck was coexpressed. themis-zap70-1 2971514 N/A 2971528 unspecified role unspecified role prey prey N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false range optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8N1K5-1:p.Tyr540_Tyr541delinsPhePhe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false whole_molecule required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false Q8N1K5-1:p.[Pro555Ala;Pro558Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5D, 5F No obvious changes in GRB2 associated to an increasingly phosphorylated THEMIS were detected. It is stated that :-"a soluble fragment of THEMIS lacking the CABIT-1 domain (aas 1-260) did not bind GRB2". However the isolated CABIT-2 domain (aas 261-641) did not bind GRB2 either. themis-grb2-3 2971515 N/A 2971528 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false SH3_C_terminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range false ecoli Escherichia coli (strain K12) N/A 2971527 unspecified role unspecified role bait bait partial_PRR1_sequence required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false Q8N1K5-1:p.Pro554Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q8N1K5-1:p.Pro557Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q8N1K5-1:p.Lys559Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q8N1K5-1:p.Arg556Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false PRR1_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) 2971562 2971555 direct interaction direct interaction false false false Figure 1 A-C Binding of full-length GRB2 and GRB2-SH3C was observed only to 29-mers containing the intact PRR1 sequence. themis-grb2-4 2971515 N/A 2971528 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false SH3_C_terminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range false ecoli Escherichia coli (strain K12) N/A 2971527 unspecified role unspecified role bait bait region_including_PRR1_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false tyr540 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false tyr541 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false Q8N1K5-1:p.Tyr540_Tyr541delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q8N1K5-1:p.Tyr540_Tyr541delinsPhePhe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Figure 5C themis-grb2-10 2971516 N/A 2971529 unspecified role unspecified role prey prey N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 2971528 unspecified role unspecified role prey prey association association false false false 2B themis-grb2-5 2971516 N/A 2971530 unspecified role unspecified role prey prey N/A 2971528 unspecified role unspecified role prey prey N/A 2971529 unspecified role unspecified role prey prey N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false 2B themis-grb2-11 2971517 N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q8N1K5-1:p.Tyr540_Tyr541delinsPhePhe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 2971528 unspecified role unspecified role prey prey physical association physical association false false false 5B themis-grb2-12 2971517 N/A 2971530 unspecified role unspecified role prey prey N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q8N1K5-1:p.Tyr540_Tyr541delinsPhePhe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 2971528 unspecified role unspecified role prey prey association association false false false 5Bi lck-themis-1 2971518 N/A 2971527 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false tyr95,tyr174,tyr220,tyr353,tyr429,tyr540, tyr541 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2971526 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation false false false Supp Table 1A P06239 (GO:0004672) up-regulates activity Q8N1K5-1 (GO:0005515) themis-zap70-2 2971518 N/A 2971525 enzyme enzyme neutral component neutral component N/A 2971527 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false tyr95,tyr174,tyr220,tyr353,tyr429,tyr540 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation false false false Supp Table 1B P43403 (GO:0004672) up-regulates activity Q8N1K5-1 (GO:0005515) lck-themis-3 2971519 N/A 2971527 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false phosphotyrosine_residues optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2971526 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation false false false Fig 5E lck-themis-2 2971519 N/A 2971528 stimulator stimulator neutral component neutral component range gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 2971527 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false phosphotyrosine_residues optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 2971526 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation false false false Fig 5Ei Addition of recombinant GRB2 reconstituted THEMIS tyrosine phosphorylation. themis-grb2-9 2971520 N/A 2971528 unspecified role unspecified role prey prey c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P62993:p.[Pro49Leu;Gly203Arg] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P62993:p.Pro49Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P62993:p.Gly203Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 1D lat-grb2-4 2971521 N/A 2971531 unspecified role unspecified role bait bait range strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O43561-2:p.[Tyr171Phe;Tyr191Phe;Tyr226Phe] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 2971532 unspecified role unspecified role prey prey N/A 2971528 unspecified role unspecified role prey prey association association false false false 2C anti-Cd3 mAb Sodium pervanadate (CHEBI:35607) Using the LAT 3F mutant as Bait, the interaction is reduced by a greater amount in anti-CD3 stimulated cells as compared to Pervanadate stimulated cells. 2342-1 2971522 N/A 2971528 unspecified role unspecified role prey prey N/A 2971533 unspecified role unspecified role bait bait c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q8BGW0:p.[Pro557Ala;Pro560Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3A themis-grb2-6 2971523 N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q8N1K5-1:p.[Pro555Ala;Pro558Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8N1K5-1:p.[Pro582Ala;Pro585Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 2971528 unspecified role unspecified role prey prey physical association physical association false false false 2A themis-plcg1-2 2971524 N/A 2971529 unspecified role unspecified role prey prey N/A 2971527 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q8N1K5-1:p.[Pro555Ala;Pro558Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2Bii