DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ li-2013-22 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252962X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-23 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252948X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-24 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252953X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-25 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252957X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-26 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252949X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-27 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252963X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-28 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252954X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-29 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252950X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-30 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252958X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-31 in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252964X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-32 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252955X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-33 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252951X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-34 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252960X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-35 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252959X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-36 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252952X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-37 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252956X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-38 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252961X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation li-2013-39 in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Li J., Richter K., Reinstein J., Buchner J. DIP original experiment: DIP-252965X Nature structural & molecular biology 2013 IMEx Integration of the accelerator Aha1 in the Hsp90 co-chaperone cycle. Li J., Richter K., Reinstein J., Buchner J. Only protein-protein interactions imex curation ppid_yeast Peptidyl-prolyl cis-trans isomerase CPR6 CPR6 YLR216C L8167.24 Rotamase CPR6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTRPKTFFDISIGGKPQGRIVFELYNDIVPKTAENFLKLCEGNAGMAKTKPDVPLSYKGSIFHRVIKDFMCQFGDFTNFNGTGGESIYDEKFEDENFTVKHDKPFLLSMANAGPNTNGSQAFITCVPTPHLDGKHVVFGEVIQGKRIVRLIENQQCDQENNKPLRDVKIDDCGVLPDDYQVPENAEATPTDEYGDNYEDVLKQDEKVDLKNFDTVLKAIETVKNIGTEQFKKQNYSVALEKYVKCDKFLKEYFPEDLEKEQIEKINQLKVSIPLNIAICALKLKDYKQVLVASSEVLYAEAADEKAKAKALYRRGLAYYHVNDTDMALNDLEMATTFQPNDAAILKAIHNTKLKRKQQNEKAKKSLSKMFS 188666D94866DDDD sti1_yeast Heat shock protein STI1 STI1 OR26.17 YOR027W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLTADEYKQQGNAAFTAKDYDKAIELFTKAIEVSETPNHVLYSNRSACYTSLKKFSDALNDANECVKINPSWSKGYNRLGAAHLGLGDLDEAESNYKKALELDASNKAAKEGLDQVHRTQQARQAQPDLGLTQLFADPNLIENLKKNPKTSEMMKDPQLVAKLIGYKQNPQAIGQDLFTDPRLMTIMATLMGVDLNMDDINQSNSMPKEPETSKSTEQKKDAEPQSDSTTSKENSSKAPQKEESKESEPMEVDEDDSKIEADKEKAEGNKFYKARQFDEAIEHYNKAWELHKDITYLNNRAAAEYEKGEYETAISTLNDAVEQGREMRADYKVISKSFARIGNAYHKLGDLKKTIEYYQKSLTEHRTADILTKLRNAEKELKKAEAEAYVNPEKAEEARLEGKEYFTKSDWPNAVKAYTEMIKRAPEDARGYSNRAAALAKLMSFPEAIADCNKAIEKDPNFVRAYIRKATAQIAVKEYASALETLDAARTKDAEVNNGSSAREIDQLYYKASQQRFQPGTSNETPEETYQRAMKDPEVAAIMQDPVMQSILQQAQQNPAALQEHMKNPEVFKKIQTLIAAGIIRTGR CF8A85C2BBD3C44C hsp82_yeast ATP-dependent molecular chaperone HSP82 HSP90 YPL240C HSP82 Heat shock protein Hsp90 heat-inducible isoform 82 kDa heat shock protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEEEEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKSVDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRDSSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETALLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVPADTEMEEVD D7C35676D668FB63 aha1_yeast Hsp90 co-chaperone AHA1 AHA1 YDR214W YD8142.16 YD8142B.06 Activator of Hsp90 ATPase protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVVNNPNNWHWVDKNCIGWAKEYFKQKLVGVEAGSVKDKKYAKIKSVSSIEGDCEVNQRKGKVISLFDLKITVLIEGHVDSKDGSALPFEGSINVPEVAFDSEASSYQFDISIFKETSELSEAKPLIRSELLPKLRQIFQQFGKDLLATHGNDIQVPESQVKSNYTRGNQKSSFTEIKDSASKPKKNALPSSTSTSAPVSSTNKVPQNGSGNSTSIYLEPTFNVPSSELYETFLDKQRILAWTRSAQFFNSGPKLETKEKFELFGGNVISELVSCEKDKKLVFHWKLKDWSAPFNSTIEMTFHESQEFHETKLQVKWTGIPVGEEDRVRANFEEYYVRSIKLTFGFGAVL 06DF6B40EB5050BE cyp7_yeast Peptidyl-prolyl cis-trans isomerase CYP7 CPR7 YJR032W J1585 Rotamase CYP7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MIQDPLVYLDISIDKKPIGRIVCKLFREKAPKTTENFYKLCAGDVKSPLKDQQYLSYKGNGFHRVVKNFMIQAGDIVFGTQKDSSSSSVGKGGCSIYADKEEVKTDDESFCYGNFEDENLGEFVEPFTLGMANLGSPNTNNSQFFITTYAAPHLNGKHSIFGQVVHGKSVVRTIENCRVDSDGVPESDVRISDCGVWEKTMGVPLYNASNDQIGGDVYEEYPDDDTHFGDDDFGKALEAANIIKESGTLLFKKKDYSNAFFKYRKSLNYINEYMPEPDVDKERNIQFINLKMKIYLNLSLVLFNLERYDDAIMYATYLLEMDNVPNRDQAKAYYRRGNSYLKKKRLDEALQDYIFCKEKNPDDEVIEQRIEYVNRLIEENKEKTRKNISKFFS 1532F255E8016F4F sba1_yeast Co-chaperone protein SBA1 YKL518 YKL117W SBA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDKVINPQVAWAQRSSTTDPERNYVLITVSIADCDAPELTIKPSYIELKAQSKPHVGDENVHHYQLHIDLYKEIIPEKTMHKVANGQHYFLKLYKKDLESEYWPRLTKEKVKYPYIKTDFDKWVDEDEQDEVEAEGNDAAQGMDFSQMMGGAGGAGGAGGMDFSQMMGGAGGAGSPDMAQLQQLLAQSGGNLDMGDFKENDEEDEEEEIEPEVKA C51CDE30BF2FA945 cpr6-hsp90-4 3250728 N/A 3250746 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250747 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false aha1-hsp90-1 3250729 N/A 3250749 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false aha1-cpr7-1 3250730 N/A 3250750 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250749 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false sti1-hsp90-2 3250731 N/A 3250747 unspecified role unspecified role fluorescence donor fluorescence donor Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3250748 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Atto550-label Atto550-label atto 550 atto 550 certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cpr6-hsp90-5 3250732 N/A 3250746 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cpr7-hsp90-1 3250733 N/A 3250750 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250747 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cpr6-aha1-2 3250734 N/A 3250746 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3250749 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3250748 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association false false false cpr7-hsp90-2 3250735 N/A 3250750 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hsp90-sti1-3 3250736 N/A 3250747 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false aha1-hsp90-2 3250737 N/A 3250749 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false aha1-hsp90-3 3250738 N/A 3250749 unspecified role unspecified role fluorescence donor fluorescence donor Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3250748 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Atto550-label Atto550-label atto 550 atto 550 certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false aha1-hsp90-4 3250739 N/A 3250749 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3250748 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false cpr6-hsp90-6 3250740 N/A 3250746 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250751 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false hsp90-p23-1 3250741 N/A 3250751 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false aha1-cpr6-1 3250742 N/A 3250746 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250749 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false hsp90-9 3250743 N/A 3250748 unspecified role unspecified role fluorescence donor fluorescence donor Alexa488-label Alexa488-label alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3250748 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Atto550-label Atto550-label atto 550 atto 550 certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cpr7-hsp90-3 3250744 N/A 3250750 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250751 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false cpr6-hsp90-7 3250745 N/A 3250746 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3250748 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false