DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ menting-2013-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252035X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions imex curation menting-2013-2 crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252040X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions imex curation menting-2013-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252037X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions imex curation menting-2013-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252039X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions imex curation menting-2013-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252036X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions imex curation menting-2013-6 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252038X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions imex curation ins_human Insulin INS protein protein human Homo sapiens Human MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver mint homomint Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730] p06213-2 Insulin receptor HIR-A INSR protein protein human Homo sapiens Human MATGGRRGAAAAPLLVAVAALLLGAAGHLYPGEVCPGMDIRNNLTRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDLFPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSVEDNYIVLNKDDNEECGDICPGTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHSECLGNCSQPDDPTKCVACRNFYLDGRCVETCPPPYYHFQDWRCVNFSFCQDLHHKCKNSRRQGCHQYVIHNNKCIPECPSGYTMNSSNLLCTPCLGPCPKVCHLLEGEKTIDSVTSAQELRGCTVINGSLIINIRGGNNLAAELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLTITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELLKFSYIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPLRSNDPKSQNHPGWLMRGLKPWTQYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVFWERQAEDSELFELDYCLKGLKLPSRTWSPPFESEDSQKHNQSEYEDSAGECCSCPKTDSQILKELEESSFRKTFEDYLHNVVFVPRPSRKRRSLGDVGNVTVAVPTVAAFPNTSSTSVPTSPEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRIRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSVVIGSIYLFLRKRQPDGPLGPLYASSNPEYLSASDVFPCSVYVPDEWEVSREKITLLRELGQGSFGMVYEGNARDIIKGEAETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSYLRSLRPEAENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDQPDNCPERVTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFHSEENKAPESEELEMEFEDMENVPLDRSSHCQREEAGGRDGGSSLGFKRSYEEHIPYTHMNGGKKNGRILTLPRSNPS ins_bovin Insulin INS protein protein bovin Bos taurus (Bovine) Bovine MALWTRLRPLLALLALWPPPPARAFVNQHLCGSHLVEALYLVCGERGFFYTPKARREVEGPQVGALELAGGPGAGGLEGPPQKRGIVEQCCASVCSLYQLENYCN ins-insr-1 3249495 N/A 3249501 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 90-110 Region 90-110 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3249502 unspecified role unspecified role unspecified role unspecified role Region 28-337 Region 28-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 731-746 Region 731-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction false false false insr-7 3249496 N/A 3249502 unspecified role unspecified role unspecified role unspecified role Region 28-337 Region 28-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction false false false ins-insr-2 3249497 N/A 3249501 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 90-110 Region 90-110 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3249502 unspecified role unspecified role unspecified role unspecified role Region 28-337 Region 28-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 724-746 Region 724-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction false false false ins-insr-3 3249498 N/A 3249503 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 85-105 Region 85-105 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bovin Bos taurus (Bovine) Bovine N/A 3249502 unspecified role unspecified role unspecified role unspecified role Region 28-620/731-746 Region 28-620/731-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction false false false ins-insr-4 3249499 N/A 3249501 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 90-110 Region 90-110 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3249502 unspecified role unspecified role unspecified role unspecified role Region 28-337 Region 28-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 731-746 Region 731-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction false false false ins-insr-5 3249500 N/A 3249503 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 85-105 Region 85-105 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bovin Bos taurus (Bovine) Bovine N/A 3249502 unspecified role unspecified role unspecified role unspecified role Region 28-620/731-746 Region 28-620/731-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction false false false