DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ guce-2013-1 drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251966X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions imex curation guce-2013-2 drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251964X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions imex curation guce-2013-3 drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251965X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions imex curation guce-2013-4 drome Drosophila melanogaster (Fruit fly) Fruit fly x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251967X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions imex curation guce-2013-5 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251968X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions imex curation guce-2013-6 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251969X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions imex curation dma_human HLA class II histocompatibility antigen, DM alpha chain HLA-DMA DMA RING6 MHC class II antigen DMA Really interesting new gene 6 protein protein protein human Homo sapiens Human MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFGKPNTLVCFVSNLFPPMLTVNWHDHSVPVEGFGPTFVSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDRYTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGIIVGIVLIIYFRKPCSGD dob_human HLA class II histocompatibility antigen, DO beta chain HLA-DOB MHC class II antigen DOB protein protein human Homo sapiens Human MGSGWVPWVVALLVNLTRLDSSMTQGTDSPEDFVIQAKADCYFTNGTEKVQFVVRFIFNLEEYVRFDSDVGMFVALTKLGQPDAEQWNSRLDLLERSRQAVDGVCRHNYRLGAPFTVGRKVQPEVTVYPERTPLLHQHNLLHCSVTGFYPGDIKIKWFLNGQEERAGVMSTGPIRNGDWTFQTVVMLEMTPELGHVYTCLVDHSSLLSPVSVEWRAQSEYSWRKMLSGIAAFLLGLIFLLVGIVIQLRAQKGYVRTQMSGNEVSRAVLLPQSC doa_human HLA class II histocompatibility antigen, DO alpha chain HLA-DOA HLA-DNA HLA-DZA MHC DN-alpha MHC DZ alpha MHC class II antigen DOA protein protein human Homo sapiens Human MALRAGLVLGFHTLMTLLSPQEAGATKADHMGSYGPAFYQSYGASGQFTHEFDEEQLFSVDLKKSEAVWRLPEFGDFARFDPQGGLAGIAAIKAHLDILVERSNRSRAINVPPRVTVLPKSRVELGQPNILICIVDNIFPPVINITWLRNGQTVTEGVAQTSFYSQPDHLFRKFHYLPFVPSAEDVYDCQVEHWGLDAPLLRHWELQVPIPPPDAMETLVCALGLAIGLVGFLVGTVLIIMGTYVSSVPR dmb_human HLA class II histocompatibility antigen, DM beta chain HLA-DMB DMB RING7 MHC class II antigen DMB Really interesting new gene 7 protein protein protein human Homo sapiens Human MITFLPLLLGLSLGCTGAGGFVAHVESTCLLDDAGTPKDFTYCISFNKDLLTCWDPEENKMAPCEFGVLNSLANVLSQHLNQKDTLMQRLRNGLQNCATHTQPFWGSLTNRTRPPSVQVAKTTPFNTREPVMLACYVWGFYPAEVTITWRKNGKLVMPHSSAHKTAQPNGDWTYQTLSHLALTPSYGDTYTCVVEHIGAPEPILRDWTPGLSPMQTLKVSVSAVTLGLGLIIFSLGVISWRRAGHSSYTPLPGSNYSEGWHIS hla_dma-hla_dmb-2 3248282 N/A 3248288 unspecified role unspecified role unspecified role unspecified role Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248289 unspecified role unspecified role unspecified role unspecified role Region 30-218 Region 30-218 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248290 unspecified role unspecified role unspecified role unspecified role Region 27-207 Region 27-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248291 unspecified role unspecified role unspecified role unspecified role Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false hla_doa-hla_dob-1 3248283 N/A 3248289 unspecified role unspecified role unspecified role unspecified role Region 30-218 Region 30-218 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248290 unspecified role unspecified role unspecified role unspecified role Region 27-207 Region 27-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false hla_dma-hla_dmb-3 3248284 N/A 3248288 unspecified role unspecified role unspecified role unspecified role Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248291 unspecified role unspecified role unspecified role unspecified role Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false hla_dma-hla_dmb-4 3248285 N/A 3248288 unspecified role unspecified role unspecified role unspecified role Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248289 unspecified role unspecified role unspecified role unspecified role Region 30-218 Region 30-218 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248290 unspecified role unspecified role unspecified role unspecified role Region 27-207 Region 27-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248291 unspecified role unspecified role unspecified role unspecified role Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false hla_dma-hla_dmb-5 3248286 N/A 3248288 unspecified role unspecified role bait bait Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248289 unspecified role unspecified role prey prey Region 30-218 Region 30-218 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248290 unspecified role unspecified role prey prey Region 27-207 Region 27-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248291 unspecified role unspecified role prey prey Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly association association false false false hla_dma-hla_dmb-6 3248287 N/A 3248288 unspecified role unspecified role bait bait Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3248291 unspecified role unspecified role prey prey Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false