DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ye-2012-9 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Ye Y., Blaser G., Horrocks MH., Ruedas-Rama MJ., Ibrahim S., Zhukov AA., Orte A., Klenerman D., Jackson SE., Komander D. DIP original experiment: DIP-252118X Nature 2012 IMEx Ubiquitin chain conformation regulates recognition and activity of interacting proteins. Ye Y., Blaser G., Horrocks MH., Ruedas-Rama MJ., Ibrahim S., Zhukov AA., Orte A., Klenerman D., Jackson SE., Komander D. Only protein-protein interactions imex curation ye-2012-10 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Ye Y., Blaser G., Horrocks MH., Ruedas-Rama MJ., Ibrahim S., Zhukov AA., Orte A., Klenerman D., Jackson SE., Komander D. DIP original experiment: DIP-252121X Nature 2012 IMEx Ubiquitin chain conformation regulates recognition and activity of interacting proteins. Ye Y., Blaser G., Horrocks MH., Ruedas-Rama MJ., Ibrahim S., Zhukov AA., Orte A., Klenerman D., Jackson SE., Komander D. Only protein-protein interactions imex curation ye-2012-11 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Ye Y., Blaser G., Horrocks MH., Ruedas-Rama MJ., Ibrahim S., Zhukov AA., Orte A., Klenerman D., Jackson SE., Komander D. DIP original experiment: DIP-252120X Nature 2012 IMEx Ubiquitin chain conformation regulates recognition and activity of interacting proteins. Ye Y., Blaser G., Horrocks MH., Ruedas-Rama MJ., Ibrahim S., Zhukov AA., Orte A., Klenerman D., Jackson SE., Komander D. Only protein-protein interactions imex curation ye-2012-12 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Ye Y., Blaser G., Horrocks MH., Ruedas-Rama MJ., Ibrahim S., Zhukov AA., Orte A., Klenerman D., Jackson SE., Komander D. DIP original experiment: DIP-252119X Nature 2012 IMEx Ubiquitin chain conformation regulates recognition and activity of interacting proteins. Ye Y., Blaser G., Horrocks MH., Ruedas-Rama MJ., Ibrahim S., Zhukov AA., Orte A., Klenerman D., Jackson SE., Komander D. Only protein-protein interactions imex curation stabp_human STAM-binding protein AMSH STAMBP Associated molecule with the SH3 domain of STAM Endosome-associated ubiquitin isopeptidase protein protein human Homo sapiens Human MSDHGDVSLPPEDRVRALSQLGSAVEVNEDIPPRRYFRSGVEIIRMASIYSEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAVIPEKKDTVKKLKEIAFPKAEELKAELLKRYTKEYTEYNEEKKKEAEELARNMAIQQELEKEKQRVAQQKQQQLEQEQFHAFEEMIRNQELEKERLKIVQEFGKVDPGLGGPLVPDLEKPSLDVFPTLTVSSIQPSDCHTTVRPAKPPVVDRSLKPGALSNSESIPTIDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVLIPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLHTHCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSKDPPLFCSCSHVTVVDRAVTITDLR ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG q96fw1-1 Ubiquitin thioesterase OTUB1 OTUB1 OTB1 OTU1 Deubiquitinating enzyme OTUB1 OTU domain-containing ubiquitin aldehyde-binding protein 1 Otubain-1 Ubiquitin-specific-processing protease OTUB1 HSPC263 Otubain-1 protein protein human Homo sapiens Human MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK q9uk80-1 Ubiquitin carboxyl-terminal hydrolase 21 USP21 USP23 Ubiquitin thioesterase 21 Ubiquitin-specific-processing protease 21 PP1490 Deubiquitinating enzyme 21 protein protein human Homo sapiens Human MPQASEHRLGRTREPPVNIQPRVGSKLPFAPRARSKERRNPASGPNPMLRPLPPRPGLPDERLKKLELGRGRTSGPRPRGPLRADHGVPLPGSPPPTVALPLPSRTNLARSKSVSSGDLRPMGIALGGHRGTGELGAALSRLALRPEPPTLRRSTSLRRLGGFPGPPTLFSIRTEPPASHGSFHMISARSSEPFYSDDKMAHHTLLLGSGHVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCEAVNPTRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPELSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFCDLSLPIPKKGFAGGKVSLRDCFNLFTKEEELESENAPVCDRCRQKTRSTKKLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLGDFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQEPPRCL ub-stambp-1 3247952 N/A 3247956 unspecified role unspecified role unspecified role unspecified role O95630:p.Glu280Ala E280A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3247957 unspecified role unspecified role fluorescence donor fluorescence donor K63Ub K63Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false Alexa488-label Alexa488-label alexa 488 label alexa 488 label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3247957 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept lys63arg K63R mutation mutation certain certain sequence position certain certain certain sequence position certain false Alexa647-label Alexa647-label alexa label alexa label n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli direct interaction direct interaction false false false ub-otub1-1 3247953 N/A 3247958 unspecified role unspecified role unspecified role unspecified role Q96FW1-1:p.Cys91Ala C91A mutation mutation certain certain sequence position certain certain certain sequence position certain false Region 40-271 Region 40-271 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3247957 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept K48Ub K48Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false Alexa647-label Alexa647-label alexa label alexa label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3247957 unspecified role unspecified role fluorescence donor fluorescence donor lys48arg K48R mutation mutation certain certain sequence position certain certain certain sequence position certain false Alexa488-label Alexa488-label alexa 488 label alexa 488 label n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli direct interaction direct interaction false false false ub-usp21-1 3247954 N/A 3247959 unspecified role unspecified role unspecified role unspecified role Region 196-565 Region 196-565 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9UK80-1:p.Cys221Ala C221A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3247957 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept K48Ub K48Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false Alexa647-label Alexa647-label alexa label alexa label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3247957 unspecified role unspecified role fluorescence donor fluorescence donor lys48arg K48R mutation mutation certain certain sequence position certain certain certain sequence position certain false Alexa488-label Alexa488-label alexa 488 label alexa 488 label n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli direct interaction direct interaction false false false ub-usp21-2 3247955 N/A 3247959 unspecified role unspecified role unspecified role unspecified role Q9UK80-1:p.Cys221Ala C221A mutation mutation certain certain sequence position certain certain certain sequence position certain false Region 196-565 Region 196-565 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3247957 unspecified role unspecified role fluorescence donor fluorescence donor K63Ub K63Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false Alexa488-label Alexa488-label alexa 488 label alexa 488 label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3247957 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept lys63arg K63R mutation mutation certain certain sequence position certain certain certain sequence position certain false Alexa647-label Alexa647-label alexa label alexa label n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli direct interaction direct interaction false false false