DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ liu-2012-28 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Liu S., Chen Y., Li J., Huang T., Tarasov S., King A., Weissman AM., Byrd RA., Das R. DIP original experiment: DIP-251343X Structure (London, England : 1993) 2012 IMEx Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains. Liu S., Chen Y., Li J., Huang T., Tarasov S., King A., Weissman AM., Byrd RA., Das R. Only protein-protein interactions imex curation liu-2012-29 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Liu S., Chen Y., Li J., Huang T., Tarasov S., King A., Weissman AM., Byrd RA., Das R. DIP original experiment: DIP-251344X Structure (London, England : 1993) 2012 IMEx Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains. Liu S., Chen Y., Li J., Huang T., Tarasov S., King A., Weissman AM., Byrd RA., Das R. Only protein-protein interactions imex curation liu-2012-30 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu S., Chen Y., Li J., Huang T., Tarasov S., King A., Weissman AM., Byrd RA., Das R. DIP original experiment: DIP-251345X Structure (London, England : 1993) 2012 IMEx Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains. Liu S., Chen Y., Li J., Huang T., Tarasov S., King A., Weissman AM., Byrd RA., Das R. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG amfr_human E3 ubiquitin-protein ligase AMFR gp78 RING finger protein 45 AMFR RNF45 Autocrine motility factor receptor protein protein human Homo sapiens Human MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS amfr-ub-1 3246680 N/A 3246683 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3246684 unspecified role unspecified role unspecified role unspecified role CUE domain Region 453-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false amfr-ub-2 3246681 N/A 3246683 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3246684 unspecified role unspecified role unspecified role unspecified role CUE domain Region 453-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false amfr-ub-3 3246682 N/A 3246683 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3246684 unspecified role unspecified role unspecified role unspecified role CUE domain Region 453-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false