DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ samanta-2012-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Samanta D., Ramagopal UA., Rubinstein R., Vigdorovich V., Nathenson SG., Almo SC. DIP original experiment: DIP-251783X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of Nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion. Samanta D., Ramagopal UA., Rubinstein R., Vigdorovich V., Nathenson SG., Almo SC. Only protein-protein interactions imex curation samanta-2012-2 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Samanta D., Ramagopal UA., Rubinstein R., Vigdorovich V., Nathenson SG., Almo SC. DIP original experiment: DIP-251784X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of Nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion. Samanta D., Ramagopal UA., Rubinstein R., Vigdorovich V., Nathenson SG., Almo SC. Only protein-protein interactions imex curation samanta-2012-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Samanta D., Ramagopal UA., Rubinstein R., Vigdorovich V., Nathenson SG., Almo SC. DIP original experiment: DIP-251785X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of Nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion. Samanta D., Ramagopal UA., Rubinstein R., Vigdorovich V., Nathenson SG., Almo SC. Only protein-protein interactions imex curation nect2_human Nectin-2 NECTIN2 HVEB Herpes virus entry mediator B Nectin cell adhesion molecule 2 PRR2 PVRL2 Poliovirus receptor-related protein 2 protein protein human Homo sapiens Human MARAAALLPSRSPPTPLLWPLLLLLLLETGAQDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTCEFATFPKGSVRGMTWLRVIAKPKNQAEAQKVTFSQDPTTVALCISKEGRPPARISWLSSLDWEAKETQVSGTLAGTVTVTSRFTLVPSGRADGVTVTCKVEHESFEEPALIPVTLSVRYPPEVSISGYDDNWYLGRTDATLSCDVRSNPEPTGYDWSTTSGTFPTSAVAQGSQLVIHAVDSLFNTTFVCTVTNAVGMGRAEQVIFVRETPNTAGAGATGGIIGGIIAAIIATAVAATGILICRQQRKEQTLQGAEEDEDLEGPPSYKPPTPKAKLEAQEMPSQLFTLGASEHSPLKTPYFDAGASCTEQEMPRYHELPTLEERSGPLHPGATSLGSPIPVPPGPPAVEDVSLDLEDEEGEEEEEYLDKINPIYDALSYSSPSDSYQGKGFVMSRAMYV nectin2-4 3244588 N/A 3244591 unspecified role unspecified role unspecified role unspecified role Region 32-158 Region 32-158 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q92692:p.Asn81Ala N81A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q92692:p.Met89Ala M89A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q92692:p.Ala143Asp A143D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q92692:p.Phe145Ala F145A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nectin2-5 3244589 N/A 3244591 unspecified role unspecified role unspecified role unspecified role Region 32-158 Region 32-158 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nectin2-6 3244590 N/A 3244591 unspecified role unspecified role unspecified role unspecified role Region 32-158 Region 32-158 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false