DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hara_yokoyama-2012-1 drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. DIP original experiment: DIP-250529X Structure (London, England : 1993) 2012 IMEx Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities. Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. Only protein-protein interactions imex curation hara_yokoyama-2012-2 crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. DIP original experiment: DIP-250532X Structure (London, England : 1993) 2012 IMEx Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities. Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. Only protein-protein interactions imex curation hara_yokoyama-2012-3 drome Drosophila melanogaster (Fruit fly) Fruit fly solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. DIP original experiment: DIP-250530X Structure (London, England : 1993) 2012 IMEx Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities. Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. Only protein-protein interactions imex curation hara_yokoyama-2012-4 drome Drosophila melanogaster (Fruit fly) Fruit fly x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. DIP original experiment: DIP-250533X Structure (London, England : 1993) 2012 IMEx Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities. Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. Only protein-protein interactions imex curation hara_yokoyama-2012-5 in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. DIP original experiment: DIP-250531X Structure (London, England : 1993) 2012 IMEx Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities. Hara-Yokoyama M., Kukimoto-Niino M., Terasawa K., Harumiya S., Podyma-Inoue KA., Hino N., Sakamoto K., Itoh S., Hashii N., Hiruta Y., Kawasaki N., Mishima-Tsumagari C., Kaitsu Y., Matsumoto T., Wakiyama M., Shirouzu M., Kasama T., Takayanagi H., Utsunomiya-Tate N., Takatsu K., Katada T., Hirabayashi Y., Yokoyama S., Yanagishita M. Only protein-protein interactions imex curation cd38_mouse ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 2'-phospho-ADP-ribosyl cyclase 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase 2'-phospho-cyclic-ADP-ribose transferase ADP-ribosyl cyclase 1 Cd38 Cyclic ADP-ribose hydrolase 1 NIM-R5 antigen I-19 protein protein mouse Mus musculus Mouse MANYEFSQVSGDRPGCRLSRKAQIGLGVGLLVLIALVVGIVVILLRPRSLLVWTGEPTTKHFSDIFLGRCLIYTQILRPEMRDQNCQEILSTFKGAFVSKNPCNITREDYAPLVKLVTQTIPCNKTLFWSKSKHLAHQYTWIQGKMFTLEDTLLGYIADDLRWCGDPSTSDMNYVSCPHWSENCPNNPITVFWKVISQKFAEDACGVVQVMLNGSLREPFYKNSTFGSVEVFSLDPNKVHKLQAWVMHDIEGASSNACSSSSLNELKMIVQKRNMIFACVDNYRPARFLQCVKNPEHPSCRLNT Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity mint cd38-1 3243165 N/A 3243170 unspecified role unspecified role unspecified role unspecified role Region 48-288 Region 48-288 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P56528:p.Gly68Glu G68E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false cd38-cd38-1 3243166 N/A 3243170 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster N/A 3243170 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster physical association physical association false false false cd38-2 3243167 N/A 3243170 unspecified role unspecified role unspecified role unspecified role Region 48-288 Region 48-288 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P56528:p.Gly68Glu G68E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false cd38-3 3243168 N/A 3243170 unspecified role unspecified role unspecified role unspecified role Region 48-288 Region 48-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P56528:p.[Asn104Asp;Asn124Asp;Asn213Asp;Asn223Asp] N104D/N124D/N213D/N223D mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false cd38-4 3243169 N/A 3243170 unspecified role unspecified role unspecified role unspecified role Region 48-288 Region 48-288 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P56528:p.Gly68Glu G68E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction false false false