DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bezerra-2012-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. DIP original experiment: DIP-251636X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Entropy-driven binding of opioid peptides induces a large domain motion in human dipeptidyl peptidase III. Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. Only protein-protein interactions imex curation bezerra-2012-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. DIP original experiment: DIP-251634X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Entropy-driven binding of opioid peptides induces a large domain motion in human dipeptidyl peptidase III. Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. Only protein-protein interactions imex curation bezerra-2012-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. DIP original experiment: DIP-251638X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Entropy-driven binding of opioid peptides induces a large domain motion in human dipeptidyl peptidase III. Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. Only protein-protein interactions imex curation bezerra-2012-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. DIP original experiment: DIP-251637X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Entropy-driven binding of opioid peptides induces a large domain motion in human dipeptidyl peptidase III. Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. Only protein-protein interactions imex curation bezerra-2012-5 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. DIP original experiment: DIP-251635X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Entropy-driven binding of opioid peptides induces a large domain motion in human dipeptidyl peptidase III. Bezerra GA., Dobrovetsky E., Viertlmayr R., Dong A., Binter A., Abramic M., Macheroux P., Dhe-Paganon S., Gruber K. Only protein-protein interactions imex curation endomorphin1 Opioid peptide endomorphin-1 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) YPWF q9ny33-1 Dipeptidyl peptidase 3 DPP3 Dipeptidyl aminopeptidase III Dipeptidyl arylamidase III Dipeptidyl peptidase III Enkephalinase B protein protein human Homo sapiens Human MADTQYILPNDIGVSSLDCREAFRLLSPTERLYAYHLSRAAWYGGLAVLLQTSPEAPYIYALLSRLFRAQDPDQLRQHALAEGLTEEEYQAFLVYAAGVYSNMGNYKSFGDTKFVPNLPKEKLERVILGSEAAQQHPEEVRGLWQTCGELMFSLEPRLRHLGLGKEGITTYFSGNCTMEDAKLAQDFLDSQNLSAYNTRLFKEVDGEGKPYYEVRLASVLGSEPSLDSEVTSKLKSYEFRGSPFQVTRGDYAPILQKVVEQLEKAKAYAANSHQGQMLAQYIESFTQGSIEAHKRGSRFWIQDKGPIVESYIGFIESYRDPFGSRGEFEGFVAVVNKAMSAKFERLVASAEQLLKELPWPPTFEKDKFLTPDFTSLDVLTFAGSGIPAGINIPNYDDLRQTEGFKNVSLGNVLAVAYATQREKLTFLEEDDKDLYILWKGPSFDVQVGLHELLGHGSGKLFVQDEKGAFNFDQETVINPETGEQIQSWYRSGETWDSKFSTIASSYEECRAESVGLYLCLHPQVLEIFGFEGADAEDVIYVNWLNMVRAGLLALEFYTPEAFNWRQAHMQARFVILRVLLEAGEGLVTITPTTGSDGRPDARVRLDRSKIRSVGKPALERFLRRLQVLKSTGDVAGGRALYEGYATVTDAPPECFLTLRDTVLLRKESRKLIVQPNTRLEGSDVQLLEYEASAAGLIRSFSERFPEDGPELEEILTQLATADARFWKGPSEAPSGQA tynorphin Opioid peptide tynorphin peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) VVYPW ivypw IVYPW peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) IVYPW leuenkephalin Opioid peptide Leu-enkephalin peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) YGGFL dpp3-endomorphin1-1 3239457 N/A 3239462 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3239463 unspecified role unspecified role unspecified role unspecified role Region 1-726 Region 1-726 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9NY33-1:p.Glu451Ala E451A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dpp3-tynorphin-1 3239458 N/A 3239464 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3239463 unspecified role unspecified role unspecified role unspecified role Region 1-726 Region 1-726 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9NY33-1:p.Glu451Ala E451A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dpp3-ivypw-1 3239459 N/A 3239465 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3239463 unspecified role unspecified role unspecified role unspecified role Region 1-726 Region 1-726 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9NY33-1:p.Glu451Ala E451A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dpp3-leuenkephalin-1 3239460 N/A 3239466 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3239463 unspecified role unspecified role unspecified role unspecified role Region 1-726 Region 1-726 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9NY33-1:p.Glu451Ala E451A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false dpp3-tynorphin-2 3239461 N/A 3239464 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3239463 unspecified role unspecified role unspecified role unspecified role Region 1-726 Region 1-726 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9NY33-1:p.Glu451Ala E451A mutation mutation certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false