DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pinotsis-2012-1 ecolx Escherichia coli atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. DIP original experiment: DIP-249362X PLoS biology 2012 IMEx Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. Only protein-protein interactions imex curation pinotsis-2012-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. DIP original experiment: DIP-249359X PLoS biology 2012 IMEx Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. Only protein-protein interactions imex curation pinotsis-2012-3 ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. DIP original experiment: DIP-249363X PLoS biology 2012 IMEx Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. Only protein-protein interactions imex curation pinotsis-2012-4 ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. DIP original experiment: DIP-249360X PLoS biology 2012 IMEx Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. Only protein-protein interactions imex curation pinotsis-2012-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. DIP original experiment: DIP-249364X PLoS biology 2012 IMEx Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. Only protein-protein interactions imex curation pinotsis-2012-6 ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. DIP original experiment: DIP-249361X PLoS biology 2012 IMEx Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. Only protein-protein interactions imex curation pinotsis-2012-7 ecolx Escherichia coli sls static light scattering sls predetermined predetermined participant predetermined Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. DIP original experiment: DIP-249365X PLoS biology 2012 IMEx Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. Pinotsis N., Chatziefthimiou SD., Berkemeier F., Beuron F., Mavridis IM., Konarev PV., Svergun DI., Morris E., Rief M., Wilmanns M. Only protein-protein interactions imex curation p52179-1 Myomesin-1 190 kDa titin-associated protein MYOM1 190 kDa connectin-associated protein Myomesin family member 1 protein protein human Homo sapiens Human MSLPFYQRCHQHYDLSYRNKDVRSTVSHYQREKKRSAVYTQGSTAYSSRSSAAHRRESEAFRRASASSSQQQASQHALSSEVSRKAASAYDYGSSHGLTDSSLLLDDYSSKLSPKPKRAKHSLLSGEEKENLPSDYMVPIFSGRQKHVSGITDTEEERIKEAAAYIAQRNLLASEEGITTSKQSTASKQTTASKQSTASKQSTASKQSTASRQSTASRQSVVSKQATSALQQEETSEKKSRKVVIREKAERLSLRKTLEETETYHAKLNEDHLLHAPEFIIKPRSHTVWEKENVKLHCSIAGWPEPRVTWYKNQVPINVHANPGKYIIESRYGMHTLEINGCDFEDTAQYRASAMNVKGELSAYASVVVKRYKGEFDETRFHAGASTMPLSFGVTPYGYASRFEIHFDDKFDVSFGREGETMSLGCRVVITPEIKHFQPEIQWYRNGVPLSPSKWVQTLWSGERATLTFSHLNKEDEGLYTIRVRMGEYYEQYSAYVFVRDADAEIEGAPAAPLDVKCLEANKDYIIISWKQPAVDGGSPILGYFIDKCEVGTDSWSQCNDTPVKFARFPVTGLIEGRSYIFRVRAVNKMGIGFPSRVSEPVAALDPAEKARLKSRPSAPWTGQIIVTEEEPSEGIVPGPPTDLSVTEATRSYVVLSWKPPGQRGHEGIMYFVEKCEAGTENWQRVNTELPVKSPRFALFDLAEGKSYCFRVRCSNSAGVGEPSEATEVTVVGDKLDIPKAPGKIIPSRNTDTSVVVSWEESKDAKELVGYYIEASVAGSGKWEPCNNNPVKGSRFTCHGLVTGQSYIFRVRAVNAAGLSEYSQDSEAIEVKAAIGGGVSPDVCPALSDEPGGLTASRGRVHEASPPTFQKDALLGSKPNKPSLPSSSQNLGQTEVSKVSETVQEELTPPPQKAAPQGKSKSDPLKKKTDRAPPSPPCDITCLESFRDSMVLGWKQPDKIGGAEITGYYVNYREVIDGVPGKWREANVKAVSEEAYKISNLKENMVYQFQVAAMNMAGLGAPSAVSECFKCEEWTIAVPGPPHSLKCSEVRKDSLVLQWKPPVHSGRTPVTGYFVDLKEAKAKEDQWRGLNEAAIKNVYLKVRGLKEGVSYVFRVRAINQAGVGKPSDLAGPVVAETRPGTKEVVVNVDDDGVISLNFECDKMTPKSEFSWSKDYVSTEDSPRLEVESKGNKTKMTFKDLGMDDLGIYSCDVTDTDGIASSYLIDEEELKRLLALSHEHKFPTVPVKSELAVEILEKGQVRFWMQAEKLSGNAKVNYIFNEKEIFEGPKYKMHIDRNTGIIEMFMEKLQDEDEGTYTFQLQDGKATNHSTVVLVGDVFKKLQKEAEFQRQEWIRKQGPHFVEYLSWEVTGECNVLLKCKVANIKKETHIVWYKDEREISVDEKHDFKDGICTLLITEFSKKDAGIYEVILKDDRGKDKSRLKLVDEAFKELMMEVCKKIALSATDLKIQSTAEGIQLYSFVTYYVEDLKVNWSHNGSAIRYSDRVKTGVTGEQIWLQINEPTPNDKGKYVMELFDGKTGHQKTVDLSGQAYDEAYAEFQRLKQAAIAEKNRARVLGGLPDVVTIQEGKALNLTCNVWGDPPPEVSWLKNEKALASDDHCNLKFEAGRTAYFTINGVSTADSGKYGLVVKNKYGSETSDFTVSVFIPEEEARMAALESLKGGKKAK myom1-5 3237226 N/A 3237233 unspecified role unspecified role unspecified role unspecified role Region 1141-1666 Region 1141-1666 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false myom1-2 3237227 N/A 3237233 unspecified role unspecified role unspecified role unspecified role Region 1352-1666 Region 1352-1666 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false myom1-3 3237228 N/A 3237233 unspecified role unspecified role unspecified role unspecified role Region 1141-1666 Region 1141-1666 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false myom1-4 3237229 N/A 3237233 unspecified role unspecified role unspecified role unspecified role Region 1141-1666 Region 1141-1666 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false myom1-6 3237230 N/A 3237233 unspecified role unspecified role unspecified role unspecified role Region 1141-1666 Region 1141-1666 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false myom1-7 3237231 N/A 3237233 unspecified role unspecified role unspecified role unspecified role Region 1141-1666 Region 1141-1666 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false myom1-8 3237232 N/A 3237233 unspecified role unspecified role unspecified role unspecified role Region 1141-1666 Region 1141-1666 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false