DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ noinaj-2012-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. DIP original experiment: DIP-249436X Nature 2012 IMEx Structural basis for iron piracy by pathogenic Neisseria. Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. Only protein-protein interactions imex curation noinaj-2012-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. DIP original experiment: DIP-249441X Nature 2012 IMEx Structural basis for iron piracy by pathogenic Neisseria. Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. Only protein-protein interactions imex curation noinaj-2012-3 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. DIP original experiment: DIP-249439X Nature 2012 IMEx Structural basis for iron piracy by pathogenic Neisseria. Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. Only protein-protein interactions imex curation noinaj-2012-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. DIP original experiment: DIP-249437X Nature 2012 IMEx Structural basis for iron piracy by pathogenic Neisseria. Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. Only protein-protein interactions imex curation noinaj-2012-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. DIP original experiment: DIP-249440X Nature 2012 IMEx Structural basis for iron piracy by pathogenic Neisseria. Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. Only protein-protein interactions imex curation noinaj-2012-6 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. DIP original experiment: DIP-249438X Nature 2012 IMEx Structural basis for iron piracy by pathogenic Neisseria. Noinaj N., Easley NC., Oke M., Mizuno N., Gumbart J., Boura E., Steere AN., Zak O., Aisen P., Tajkhorshid E., Evans RW., Gorringe AR., Mason AB., Steven AC., Buchanan SK. Only protein-protein interactions imex curation trfe_human Serotransferrin Siderophilin Beta-1 metal-binding globulin TF PRO1400 protein protein human Homo sapiens Human MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP tbpa_neimb Transferrin-binding protein A tbp1 Transferrin-binding protein 1 tbpA NMB0461 protein protein strain mc58 Neisseria meningitidis serogroup B MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKAKKQKTRRDNEVTGLGKLVKSSDTLSKEQVLNIRDLTRYDPGIAVVEQGRGASSGYSIRGMDKNRVSLTVDGVSQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSVEQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEALLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDSSNYAYFIVKEECKNGSYETCKANPKKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHTQQTFDTRDMTVPAFLTKAVFDANKKQAGSLPGNGKYAGNHKYGGLFTNGENGALVGAEYGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGSDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSNLRHQDYYYQHANRAYSSNTPPQNNGKKISPNGSETSPYWVTIGRGNVVTGQICRLGNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSWNAGIVLKPTDWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVFKGDFGNLEASWFNNAYRDLIVRGYEAQIKDGKEEAKGDPAYLNAQSARITGINILGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTVKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF 110A3F38A59287D5 tbpb_neimb Transferrin-binding protein B tbpB tbp2 NMB0460 Surface lipoprotein TbpB Transferrin-binding protein 2 protein protein strain mc58 Neisseria meningitidis serogroup B MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKYQDVFSEKPQAQKDQGGYGFAMRLKRRNWYPQAKEDEVKLDESDWEATGLPDEPKELPKRQKSVIEKVETDSDNNIYSSPYLKPSNHQNGNTGNGINQPKNQAKDYENFKYVYSGWFYKHAKREFNLKVEPKSAKNGDDGYIFYHGKEPSRQLPASGKITYKGVWHFATDTKKGQKFREIIQPSKSQGDRYSGFSGDDGEEYSNKNKSTLTDGQEGYGFTSNLEVDFHNKKLTGKLIRNNANTDNNQATTTQYYSLEAQVTGNRFNGKATATDKPQQNSETKEHPFVSDSSSLSGGFFGPQGEELGFRFLSDDQKVAVVGSAKTKDKPANGNTAAASGGTDAAASNGAAGTSSENGKLTTVLDAVELKLGDKEVQKLDNFSNAAQLVVDGIMIPLLPEASESGNNQANQGTNGGTAFTRKFDHTPESDKKDAQAGTQTNGAQTASNTAGDTNGKTKTYEVEVCCSNLNYLKYGMLTRKNSKSAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIPSEQNIVYRGSWYGYIANDKSTSWSGNASNATSGNRAEFTVNFADKKITGTLTADNRQEATFTIDGNIKDNGFEGTAKTAESGFDLDQSNTTRTPKAYITDAKVQGGFYGPKAEELGGWFAYPGDKQTKNATNASGNSSATVVFGAKRQQPVR 77EA248941E8EF0C tbpa-tf-1 3236540 N/A 3236546 unspecified role unspecified role unspecified role unspecified role C-lobe domain Region 356-698 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3236547 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tbpa-tbpb-1 3236541 N/A 3236548 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3236546 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3236547 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false tbpb-tf-1 3236542 N/A 3236548 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3236546 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false tbpa-tf-2 3236543 N/A 3236546 unspecified role unspecified role unspecified role unspecified role C-lobe domain Region 356-698 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3236547 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tbpb-tf-2 3236544 N/A 3236548 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3236546 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false tbpa-tbpb-2 3236545 N/A 3236548 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3236546 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3236547 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false