DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chang-2012-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. DIP original experiment: DIP-250804X Nature structural & molecular biology 2012 IMEx Structural characterization of full-length NSF and 20S particles. Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. Only protein-protein interactions imex curation chang-2012-3 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. DIP original experiment: DIP-250805X Nature structural & molecular biology 2012 IMEx Structural characterization of full-length NSF and 20S particles. Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. Only protein-protein interactions imex curation chang-2012-4 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. DIP original experiment: DIP-250806X Nature structural & molecular biology 2012 IMEx Structural characterization of full-length NSF and 20S particles. Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. Only protein-protein interactions imex curation chang-2012-5 in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. DIP original experiment: DIP-250808X Nature structural & molecular biology 2012 IMEx Structural characterization of full-length NSF and 20S particles. Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. Only protein-protein interactions imex curation chang-2012-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. DIP original experiment: DIP-250807X Nature structural & molecular biology 2012 IMEx Structural characterization of full-length NSF and 20S particles. Chang LF., Chen S., Liu CC., Pan X., Jiang J., Bai XC., Xie X., Wang HW., Sui SF. Only protein-protein interactions imex curation nsf_crigr Vesicle-fusing ATPase NSF N-ethylmaleimide-sensitive fusion protein Vesicular-fusion protein NSF protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MAGRSMQAARCPTDELSLSNCAVVSEKDYQSGQHVIVRTSPNHKYIFTLRTHPSVVPGSVAFSLPQRKWAGLSIGQEIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD snp25_rat Synaptosomal-associated protein 25 Snap25 Snap Synaptosomal-associated 25 kDa protein Super protein protein protein rat Rattus norvegicus (Rat) Rat MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG stx1a_rat Syntaxin-1A Synaptotagmin-associated 35 kDa protein Neuron-specific antigen HPC-1 Stx1a Sap protein protein rat Rattus norvegicus (Rat) Rat MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKKIMIIICCVILGIIIASTIGGIFG vamp2_rat Vesicle-associated membrane protein 2 Vamp2 Syb2 Synaptobrevin-2 protein protein rat Rattus norvegicus (Rat) Rat MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST nsf-3 3235900 N/A 3235905 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false nsf-4 3235901 N/A 3235905 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false nsf-5 3235902 N/A 3235905 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false snap25-snap25-7 3235903 N/A 3235906 unspecified role unspecified role unspecified role unspecified role Region 125-206 Region 125-206 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235907 unspecified role unspecified role unspecified role unspecified role Region 2-253 Region 2-253 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235906 unspecified role unspecified role unspecified role unspecified role Region 1-100 Region 1-100 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235908 unspecified role unspecified role unspecified role unspecified role Region 1-94 Region 1-94 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false snap25-snap25-8 3235904 N/A 3235906 unspecified role unspecified role unspecified role unspecified role Region 125-206 Region 125-206 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235907 unspecified role unspecified role unspecified role unspecified role Region 2-253 Region 2-253 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235906 unspecified role unspecified role unspecified role unspecified role Region 1-100 Region 1-100 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3235908 unspecified role unspecified role unspecified role unspecified role Region 1-94 Region 1-94 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false