DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ li-2012-15 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. DIP original experiment: DIP-249339X PLoS biology 2012 IMEx Hedgehog-regulated ubiquitination controls smoothened trafficking and cell surface expression in Drosophila. Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. Only protein-protein interactions imex curation li-2012-16 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. DIP original experiment: DIP-249342X PLoS biology 2012 IMEx Hedgehog-regulated ubiquitination controls smoothened trafficking and cell surface expression in Drosophila. Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. Only protein-protein interactions imex curation li-2012-17 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. DIP original experiment: DIP-249341X PLoS biology 2012 IMEx Hedgehog-regulated ubiquitination controls smoothened trafficking and cell surface expression in Drosophila. Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. Only protein-protein interactions imex curation li-2012-18 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. DIP original experiment: DIP-249343X PLoS biology 2012 IMEx Hedgehog-regulated ubiquitination controls smoothened trafficking and cell surface expression in Drosophila. Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. Only protein-protein interactions imex curation li-2012-19 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. DIP original experiment: DIP-249340X PLoS biology 2012 IMEx Hedgehog-regulated ubiquitination controls smoothened trafficking and cell surface expression in Drosophila. Li S., Chen Y., Shi Q., Yue T., Wang B., Jiang J. Only protein-protein interactions imex curation ubiq_drome Ubiquitin RpL40 CG2960 RpS27A UBI-F80 Ubi-f CG5271 Ubi-p63E Ubi-m CG11624 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG smo_drome Protein smoothened smo CG11561 dSMO SMOH Smooth protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQYLNFPRMPNIMMFLEVAILCLWVVADASASSAKFGSTTPASAQQSDVELEPINGTLNYRLYAKKGRDDKPWFDGLDSRHIQCVRRARCYPTSNATNTCFGSKLPYELSSLDLTDFHTEKELNDKLNDYYALKHVPKCWAAIQPFLCAVFKPKCEKINGEDMVYLPSYEMCRITMEPCRILYNTTFFPKFLRCNETLFPTKCTNGARGMKFNGTGQCLSPLVPTDTSASYYPGIEGCGVRCKDPLYTDDEHRQIHKLIGWAGSICLLSNLFVVSTFFIDWKNANKYPAVIVFYINLCFLIACVGWLLQFTSGSREDIVCRKDGTLRHSEPTAGENLSCIVIFVLVYYFLTAGMVWFVFLTYAWHWRAMGHVQDRIDKKGSYFHLVAWSLPLVLTITTMAFSEVDGNSIVGICFVGYINHSMRAGLLLGPLCGVILIGGYFITRGMVMLFGLKHFANDIKSTSASNKIHLIIMRMGVCALLTLVFILVAIACHVTEFRHADEWAQSFRQFIICKISSVFEEKSSCRIENRPSVGVLQLHLLCLFSSGIVMSTWCWTPSSIETWKRYIRKKCGKEVVEEVKMPKHKVIAQTWAKRKDFEDKGRLSITLYNTHTDPVGLNFDVNDLNSSETNDISSTWAAYLPQCVKRRMALTGAATGNSSSHGPRKNSLDSEISVSVRHVSVESRRNSVDSQVSVKIAEMKTKVASRSRGKHGGSSSNRRTQRRRDYIAAATGKSSRRRESSTSVESQVIALKKTTYPNASHKVGVFAHHSSKKQHNYTSSMKRRTANAGLDPSILNEFLQKNGDFIFPFLQNQDMSSSSEEDNSRASQKIQDLNVVVKQQEISEDDHDGIKIEELPNSKQVALENFLKNIKKSNESNSNRHSRNSARSQSKKSQKRHLKNPAADLDFRKDCVKYRSNDSLSCSSEELDVALDVGSLLNSSFSGISMGKPHSRNSKTSCDVGIQANPFELVPSYGEDELQQAMRLLNAASRQRTEAANEDFGGTELQGLLGHSHRHQREPTFMSESDKLKMLLLPSK q9v393_drome krz CG1487 0952 14 Dmel\CG1487 Krz Kurz l(3)S095214 Dmel_CG1487 beta-arr2 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNGGGGGSGGANVSNGSSTNVTAAGSTGGGSAGDETGGDASSRRQATRVFKKSSSNGKITVYLGKRDFVDHVTHVDPIDGVVFIDPEYVKDRKVFGQVLAAFRYGREDLDVLGLTFRKDLYLAHEQIYPPMQLDRPMTRLQERLIKKLGPNAHPFYFEVPPYCPASVSLQPAPGDVGKSCGVDYELKAFVGENVEDKPHKRNSVRLTIRKVMYAPSKVGEQPSIEVSKEFMMKPNKIHLEATLDKELYHHGEKISVNVHVANNSNRTVKKIKVCVRQFADICLFSTAQYKSVVAEIESEDGCQVAPGFTLSKVFELCPLLANNKDKWGLALDGQLKHEDTNLASSTLITNPAQRESLGIMVHYKVKVKLLISSPLLNGDLVAELPFTLMHPKPEEEEHPLLGERSPRASLAGGGLPLVSMSDGETESATGGQDVPTTTNLIQLDDDEAQDDDIIFEDFARLRLKGAETEA q9vdd8_drome Ubiquitin carboxyl-terminal hydrolase Usp8 Ubpy Ubpy-RA CT18196 Dmel\CG5798 Q9VDD8 UBPY USP8 UbpY dUBPY ubpy usp8 Dmel_CG5798 CG5798 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAKLKKLHMSSNLDDLEKMSIIPDLRSKGMKILLNTARKLYMTAEEYRLDGDEELAYITYMKYFNMLTAIHKKSDYPSHKTTVRQMLGDTESNRRIMDTLEEIINSLRHRYAQQHQAPEPIAPDLVSNGRAGVDSPITQPTQYARLGLITCQDLYRRMQEKSVLVMDCRPSADYEASHLTYYCAFNVPEELITPGMSAGRLQARLSSSAKASWASRSVKDSVVLMDWNTKDAQPATNTAISTLLDILKNWDPDVTYRAPIQIVEGGYEYFIMMYPTHCTNPSVQAPQQNNNDIETIDDIEYPSIHDITMKEDISAKDFRPRPDFNRANKPAATRVNEQGISRPSPPAKPIAEIMRDQAEFLQRAEQNDEQLEKASKMWKRQAAEGDGLNATEDQELHFRILQLESKAQDYIVENNRLREELSRIQELHNVTQQLSQKEVEATRNIESKIRERQRLDEQHELERQERERLLAIARETKKHYKSPTPSGPPSPGRNLEDVHVVSDSLESLLQLTGDPDPTIAPNKAEIPTFDRAMKPQPRNVERTSQRVRDFSPVIGQNVGRGLTGLKNLGNTCYMNSILQCLSNTPQLTEYCISDKYKNYISRSNKTNGQVIEEVAALIKELWNGQYKCVASRDLRYVVGQYQKIFRGVDQQDSHEFLTILMDWLHSDLQTLHVPRQREMISASEKAWLEFTKAKESMILHLFYGQMKSTVKCVACHKESATYESFSNLSLELPPNSNVCQLNQCMDMYFSGERIHGWNCPSCKTKRDAIKKLDISKLPPVLVVHLKRFYADPSNSGSYMKKQNYLRFPLENLDMNPYIARAESRAVTPKTYQLYAVSNHYGTMEGGHYTAFCKSANYGKWFKFDDQVVSALDSSNVVSSAAYILFYTWLPPMQVPL friz2_drome Frizzled-2 CG9739 fz2 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MRHNRLKVLILGLVLLLTSCRADGPLHSADHGMGGMGMGGHGLDASPAPGYGVPVIPKDPNLRCEEITIPMCRGIGYNMTSFPNEMNHETQDEAGLEVHQFWPLVEIKCSPDLKFFLCSMYTPICLEDYHKPLPVCRSVCERARSGCAPIMQQYSFEWPERMACEHLPLHGDPDNLCMEQPSYTEAGSGGSSGGSGGSGSGSGSGGKRKQGGSGSGGSGAGGSSGSTSTKPCRGRNSKNCQNPQGEKASGKECSCSCRSPLIFLGKEQLLQQQSQMPMMHHPHHWYMNLTVQRIAGVPNCGIPCKGPFFSNDEKDFAGLWIALWSGLCFCSTLMTLTTFIIDTERFKYPERPIVFLSACYFMVAVGYLSRNFLQNEEIACDGLLLRESSTGPHSCTLVFLLTYFFGMASSIWWVILSFTWFLAAGLKWGNEAITKHSQYFHLAAWLIPTVQSVAVLLLSAVDGDPILGICYVGNLNPDHLKTFVLAPLFVYLVIGTTFLMAGFVSLFRIRSVIKQQGGVGAGVKADKLEKLMIRIGIFSVLYTVPATIVIGCYLYEAAYFEDWIKALACPCAQVKGPGKKPLYSVLMLKYFMALAVGITSGVWIWSGKTLESWRRFWRRLLGAPDRTGANQALIKQRPPIPHPYAGSGMGMPVGSAAGSLLATPYTQAGGASVASTSHHHLHHHVLKQPAASHV smo-ub-1 3235440 N/A 3235445 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235446 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P91682:p.[Ser667Asp;Ser670Asp;Ser673Asp;Ser687Asp;Ser690Asp;Ser693Asp;Ser740Asp;Ser743Asp;Ser746Asp] S667D/S670D/S673D/S687D/S690D/S693D/S740D/S743D/S746D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P91682:p.[Lys665Arg;Lys695Arg;Lys700Arg;Lys702Arg;Lys710Arg;Lys733Arg;Lys752Arg;Lys753Arg;Lys762Arg;Lys772Arg;Lys773Arg;Lys782Arg;Lys801Arg] K665R/K695R/K700R/K702R/K710R/K733R/K752R/K753R/K762R/K772R/K773R/K782R/K801R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P91682:p.[Lys665Arg;Lys695Arg;Lys700Arg;Lys702Arg;Lys710Arg;Lys733Arg] K665R/K695R/K700R/K702R/K710R/K733R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P91682:p.[Lys752_Lys753delinsArgArg;Lys762Arg;Lys772_Lys773delinsArgArg;Lys782Arg;Lys801Arg] K752R/K753R/K762R/K772R/K773R/K782R/K801R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false Deletion of region 661-818 decreased the interaction rate/strength. smo-krz-1 3235441 N/A 3235447 unspecified role unspecified role prey prey YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235446 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false C-terminal domain Region 556-1036 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P91682:p.[Ser667Asp;Ser670Asp;Ser673Asp;Ser687Asp;Ser690Asp;Ser693Asp;Ser740Asp;Ser743Asp;Ser746Asp] S667D/S670D/S673D/S687D/S690D/S693D/S740D/S743D/S746D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false smo-usp8-4 3235442 N/A 3235448 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235446 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false C-terminal domain Region 556-1036 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false fz2-ub-1 3235443 N/A 3235449 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235445 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false smo-ub-2 3235444 N/A 3235445 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235446 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P91682:p.[Lys665Arg;Lys695Arg;Lys700Arg;Lys702Arg;Lys710Arg;Lys733Arg;Lys752Arg;Lys753Arg;Lys762Arg;Lys772Arg;Lys773Arg;Lys782Arg;Lys801Arg] K665R/K695R/K700R/K702R/K710R/K733R/K752R/K753R/K762R/K772R/K773R/K782R/K801R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P91682:p.[Lys752_Lys753delinsArgArg;Lys762Arg;Lys772_Lys773delinsArgArg;Lys782Arg;Lys801Arg] K752R/K753R/K762R/K772R/K773R/K782R/K801R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P91682:p.[Lys665Arg;Lys695Arg;Lys700Arg;Lys702Arg;Lys710Arg;Lys733Arg] K665R/K695R/K700R/K702R/K710R/K733R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false