DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ xia-2012-1 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xia R., Jia H., Fan J., Liu Y., Jia J. DIP original experiment: DIP-249334X PLoS biology 2012 IMEx USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. Xia R., Jia H., Fan J., Liu Y., Jia J. Only protein-protein interactions imex curation xia-2012-2 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xia R., Jia H., Fan J., Liu Y., Jia J. DIP original experiment: DIP-249332X PLoS biology 2012 IMEx USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. Xia R., Jia H., Fan J., Liu Y., Jia J. Only protein-protein interactions imex curation xia-2012-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Xia R., Jia H., Fan J., Liu Y., Jia J. DIP original experiment: DIP-249336X PLoS biology 2012 IMEx USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. Xia R., Jia H., Fan J., Liu Y., Jia J. Only protein-protein interactions imex curation xia-2012-4 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xia R., Jia H., Fan J., Liu Y., Jia J. DIP original experiment: DIP-249335X PLoS biology 2012 IMEx USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. Xia R., Jia H., Fan J., Liu Y., Jia J. Only protein-protein interactions imex curation xia-2012-5 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xia R., Jia H., Fan J., Liu Y., Jia J. DIP original experiment: DIP-249333X PLoS biology 2012 IMEx USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. Xia R., Jia H., Fan J., Liu Y., Jia J. Only protein-protein interactions imex curation xia-2012-6 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xia R., Jia H., Fan J., Liu Y., Jia J. DIP original experiment: DIP-249338X PLoS biology 2012 IMEx USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. Xia R., Jia H., Fan J., Liu Y., Jia J. Only protein-protein interactions imex curation xia-2012-7 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xia R., Jia H., Fan J., Liu Y., Jia J. DIP original experiment: DIP-249337X PLoS biology 2012 IMEx USP8 promotes smoothened signaling by preventing its ubiquitination and changing its subcellular localization. Xia R., Jia H., Fan J., Liu Y., Jia J. Only protein-protein interactions imex curation ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG smo_human Smoothened homolog Protein Gx SMO SMOH protein protein human Homo sapiens Human MAAARPARGPELPLLGLLLLLLLGDPGRGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRLGEPTSNETLSCVIIFVIVYYALMAGVVWFVVLTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKQPIPDCEIKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGQSDDEPKRIKKSKMIAKAFSKRHELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQHVTKMVARRGAILPQDISVTPVATPVPPEEQANLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIPRLPQLPRQKCLVAAGAWGAGDSCRQGAWTLVSNPFCPEPSPPQDPFLPSAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF ubiq_drome Ubiquitin RpL40 CG2960 RpS27A UBI-F80 Ubi-f CG5271 Ubi-p63E Ubi-m CG11624 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG smo_drome Protein smoothened smo CG11561 dSMO SMOH Smooth protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQYLNFPRMPNIMMFLEVAILCLWVVADASASSAKFGSTTPASAQQSDVELEPINGTLNYRLYAKKGRDDKPWFDGLDSRHIQCVRRARCYPTSNATNTCFGSKLPYELSSLDLTDFHTEKELNDKLNDYYALKHVPKCWAAIQPFLCAVFKPKCEKINGEDMVYLPSYEMCRITMEPCRILYNTTFFPKFLRCNETLFPTKCTNGARGMKFNGTGQCLSPLVPTDTSASYYPGIEGCGVRCKDPLYTDDEHRQIHKLIGWAGSICLLSNLFVVSTFFIDWKNANKYPAVIVFYINLCFLIACVGWLLQFTSGSREDIVCRKDGTLRHSEPTAGENLSCIVIFVLVYYFLTAGMVWFVFLTYAWHWRAMGHVQDRIDKKGSYFHLVAWSLPLVLTITTMAFSEVDGNSIVGICFVGYINHSMRAGLLLGPLCGVILIGGYFITRGMVMLFGLKHFANDIKSTSASNKIHLIIMRMGVCALLTLVFILVAIACHVTEFRHADEWAQSFRQFIICKISSVFEEKSSCRIENRPSVGVLQLHLLCLFSSGIVMSTWCWTPSSIETWKRYIRKKCGKEVVEEVKMPKHKVIAQTWAKRKDFEDKGRLSITLYNTHTDPVGLNFDVNDLNSSETNDISSTWAAYLPQCVKRRMALTGAATGNSSSHGPRKNSLDSEISVSVRHVSVESRRNSVDSQVSVKIAEMKTKVASRSRGKHGGSSSNRRTQRRRDYIAAATGKSSRRRESSTSVESQVIALKKTTYPNASHKVGVFAHHSSKKQHNYTSSMKRRTANAGLDPSILNEFLQKNGDFIFPFLQNQDMSSSSEEDNSRASQKIQDLNVVVKQQEISEDDHDGIKIEELPNSKQVALENFLKNIKKSNESNSNRHSRNSARSQSKKSQKRHLKNPAADLDFRKDCVKYRSNDSLSCSSEELDVALDVGSLLNSSFSGISMGKPHSRNSKTSCDVGIQANPFELVPSYGEDELQQAMRLLNAASRQRTEAANEDFGGTELQGLLGHSHRHQREPTFMSESDKLKMLLLPSK q9vdd8_drome Ubiquitin carboxyl-terminal hydrolase Usp8 Ubpy Ubpy-RA CT18196 Dmel\CG5798 Q9VDD8 UBPY USP8 UbpY dUBPY ubpy usp8 Dmel_CG5798 CG5798 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAKLKKLHMSSNLDDLEKMSIIPDLRSKGMKILLNTARKLYMTAEEYRLDGDEELAYITYMKYFNMLTAIHKKSDYPSHKTTVRQMLGDTESNRRIMDTLEEIINSLRHRYAQQHQAPEPIAPDLVSNGRAGVDSPITQPTQYARLGLITCQDLYRRMQEKSVLVMDCRPSADYEASHLTYYCAFNVPEELITPGMSAGRLQARLSSSAKASWASRSVKDSVVLMDWNTKDAQPATNTAISTLLDILKNWDPDVTYRAPIQIVEGGYEYFIMMYPTHCTNPSVQAPQQNNNDIETIDDIEYPSIHDITMKEDISAKDFRPRPDFNRANKPAATRVNEQGISRPSPPAKPIAEIMRDQAEFLQRAEQNDEQLEKASKMWKRQAAEGDGLNATEDQELHFRILQLESKAQDYIVENNRLREELSRIQELHNVTQQLSQKEVEATRNIESKIRERQRLDEQHELERQERERLLAIARETKKHYKSPTPSGPPSPGRNLEDVHVVSDSLESLLQLTGDPDPTIAPNKAEIPTFDRAMKPQPRNVERTSQRVRDFSPVIGQNVGRGLTGLKNLGNTCYMNSILQCLSNTPQLTEYCISDKYKNYISRSNKTNGQVIEEVAALIKELWNGQYKCVASRDLRYVVGQYQKIFRGVDQQDSHEFLTILMDWLHSDLQTLHVPRQREMISASEKAWLEFTKAKESMILHLFYGQMKSTVKCVACHKESATYESFSNLSLELPPNSNVCQLNQCMDMYFSGERIHGWNCPSCKTKRDAIKKLDISKLPPVLVVHLKRFYADPSNSGSYMKKQNYLRFPLENLDMNPYIARAESRAVTPKTYQLYAVSNHYGTMEGGHYTAFCKSANYGKWFKFDDQVVSALDSSNVVSSAAYILFYTWLPPMQVPL smo-mub-1 3235386 N/A 3235393 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3235394 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse physical association physical association false false false smo-dub-1 3235387 N/A 3235395 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235396 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P91682:p.[Ser667Asp;Ser670Asp;Ser673Asp;Ser687Asp;Ser690Asp;Ser693Asp;Ser740Asp;Ser743Asp;Ser746Asp] S667D/S670D/S673D/S687D/S690D/S693D/S740D/S743D/S746D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false smo-usp8-1 3235388 N/A 3235397 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235396 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P91682:p.[Ser667Asp;Ser670Asp;Ser673Asp;Ser687Asp;Ser690Asp;Ser693Asp;Ser740Asp;Ser743Asp;Ser746Asp] S667D/S670D/S673D/S687D/S690D/S693D/S740D/S743D/S746D mutation increasing mutation increasing interaction mutation increasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Region 625-753 Region 625-753 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false smo-usp8-2 3235389 N/A 3235397 unspecified role unspecified role prey prey Region 536-896 Region 536-896 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235396 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false C-terminal domain Region 556-1036 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P91682:p.[Ser667Asp;Ser670Asp;Ser673Asp;Ser687Asp;Ser690Asp;Ser693Asp;Ser740Asp;Ser743Asp;Ser746Asp] S667D/S670D/S673D/S687D/S690D/S693D/S740D/S743D/S746D mutation increasing mutation increasing interaction mutation increasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P91682:p.[Ser667Ala;Ser687Ala;Ser740Ala] S667A/S687A/S740A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false smo-dub-2 3235390 N/A 3235395 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235396 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false smo-dub-3 3235391 N/A 3235395 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3235394 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false smo-usp8-3 3235392 N/A 3235397 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3235396 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 556-730 Region 556-730 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 679-1036 Region 679-1036 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false