DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jacob-2012-1 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jacob CO., Eisenstein M., Dinauer MC., Ming W., Liu Q., John S., Quismorio FP., Reiff A., Myones BL., Kaufman KM., McCurdy D., Harley JB., Silverman E., Kimberly RP., Vyse TJ., Gaffney PM., Moser KL., Klein-Gitelman M., Wagner-Weiner L., Langefeld CD., Armstrong DL., Zidovetzki R. DIP original experiment: DIP-251459X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Lupus-associated causal mutation in neutrophil cytosolic factor 2 (NCF2) brings unique insights to the structure and function of NADPH oxidase. Jacob CO., Eisenstein M., Dinauer MC., Ming W., Liu Q., John S., Quismorio FP., Reiff A., Myones BL., Kaufman KM., McCurdy D., Harley JB., Silverman E., Kimberly RP., Vyse TJ., Gaffney PM., Moser KL., Klein-Gitelman M., Wagner-Weiner L., Langefeld CD., Armstrong DL., Zidovetzki R. Only protein-protein interactions imex curation jacob-2012-2 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jacob CO., Eisenstein M., Dinauer MC., Ming W., Liu Q., John S., Quismorio FP., Reiff A., Myones BL., Kaufman KM., McCurdy D., Harley JB., Silverman E., Kimberly RP., Vyse TJ., Gaffney PM., Moser KL., Klein-Gitelman M., Wagner-Weiner L., Langefeld CD., Armstrong DL., Zidovetzki R. DIP original experiment: DIP-251461X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Lupus-associated causal mutation in neutrophil cytosolic factor 2 (NCF2) brings unique insights to the structure and function of NADPH oxidase. Jacob CO., Eisenstein M., Dinauer MC., Ming W., Liu Q., John S., Quismorio FP., Reiff A., Myones BL., Kaufman KM., McCurdy D., Harley JB., Silverman E., Kimberly RP., Vyse TJ., Gaffney PM., Moser KL., Klein-Gitelman M., Wagner-Weiner L., Langefeld CD., Armstrong DL., Zidovetzki R. Only protein-protein interactions imex curation jacob-2012-3 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jacob CO., Eisenstein M., Dinauer MC., Ming W., Liu Q., John S., Quismorio FP., Reiff A., Myones BL., Kaufman KM., McCurdy D., Harley JB., Silverman E., Kimberly RP., Vyse TJ., Gaffney PM., Moser KL., Klein-Gitelman M., Wagner-Weiner L., Langefeld CD., Armstrong DL., Zidovetzki R. DIP original experiment: DIP-251460X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Lupus-associated causal mutation in neutrophil cytosolic factor 2 (NCF2) brings unique insights to the structure and function of NADPH oxidase. Jacob CO., Eisenstein M., Dinauer MC., Ming W., Liu Q., John S., Quismorio FP., Reiff A., Myones BL., Kaufman KM., McCurdy D., Harley JB., Silverman E., Kimberly RP., Vyse TJ., Gaffney PM., Moser KL., Klein-Gitelman M., Wagner-Weiner L., Langefeld CD., Armstrong DL., Zidovetzki R. Only protein-protein interactions imex curation jacob-2012-4 chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jacob CO., Eisenstein M., Dinauer MC., Ming W., Liu Q., John S., Quismorio FP., Reiff A., Myones BL., Kaufman KM., McCurdy D., Harley JB., Silverman E., Kimberly RP., Vyse TJ., Gaffney PM., Moser KL., Klein-Gitelman M., Wagner-Weiner L., Langefeld CD., Armstrong DL., Zidovetzki R. DIP original experiment: DIP-251462X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Lupus-associated causal mutation in neutrophil cytosolic factor 2 (NCF2) brings unique insights to the structure and function of NADPH oxidase. Jacob CO., Eisenstein M., Dinauer MC., Ming W., Liu Q., John S., Quismorio FP., Reiff A., Myones BL., Kaufman KM., McCurdy D., Harley JB., Silverman E., Kimberly RP., Vyse TJ., Gaffney PM., Moser KL., Klein-Gitelman M., Wagner-Weiner L., Langefeld CD., Armstrong DL., Zidovetzki R. Only protein-protein interactions imex curation ncf2_human Neutrophil cytosol factor 2 NOXA2 P67PHOX Neutrophil NADPH oxidase factor 2 NADPH oxidase activator 2 67 kDa neutrophil oxidase factor p67-phox NCF2 protein protein human Homo sapiens Human MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAFTRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVGKLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV vav_mouse Proto-oncogene vav Vav1 p95vav Vav protein protein mouse Mus musculus Mouse MELWRQCTHWLIQCRVLPPSHRVTWEGAQVCELAQALRDGVLLCQLLNNLLPQAINLREVNLRPQMSQFLCLKNIRTFLSTCCEKFGLKRSELFEAFDLFDVQDFGKVIYTLSALSWTPIAQNKGIMPFPTEDSALNDEDIYSGLSDQIDDTAEEDEDLYDCVENEEAEGDEIYEDLMRLESVPTPPKMTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFMKPLQRFLKPQDMETIFVNIEELFSVHTHFLKELKDALAGPGATTLYQVFIKYKERFLVYGRYCSQVESASKHLDQVATAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQDATEKENLRLALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLANYGRPKIDGELKITSVERRSKTDRYAFLLDKALLICKRRGDSYDLKASVNLHSFQVRDDSSGERDNKKWSHMFLLIEDQGAQGYELFFKTRELKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCYRCRAPAHKECLGRVPPCGRHGQDFAGTMKKDKLHRRAQDKKRNELGLPKMEVFQEYYGIPPPPGAFGPFLRLNPGDIVELTKAEAEHNWWEGRNTATNEVGWFPCNRVHPYVHGPPQDLSVHLWYAGPMERAGAEGILTNRSDGTYLVRQRVKDTAEFAISIKYNVEVKHIKIMTSEGLYRITEKKAFRGLLELVEFYQQNSLKDCFKSLDTTLQFPYKEPERRAISKPPAGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYGRIGWFPSNYVEEDYSEYC vav1-ncf2-1 3234653 N/A 3234657 unspecified role unspecified role prey prey Region 351-526 Region 351-526 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 1-400 Region 1-400 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 299-526 Region 299-526 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3234658 unspecified role unspecified role bait bait chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false vav1-ncf2-2 3234654 N/A 3234657 unspecified role unspecified role prey prey chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3234658 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-607 Region 1-607 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false vav1-ncf2-3 3234655 N/A 3234657 unspecified role unspecified role prey prey chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3234658 unspecified role unspecified role bait bait Region 1-608 Region 1-608 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 531-845 Region 531-845 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false vav1-ncf2-4 3234656 N/A 3234657 unspecified role unspecified role prey prey chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3234658 unspecified role unspecified role bait bait EGFP-tag EGFP-tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 187-607 Region 187-607 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false