DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ patino-2011-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. DIP original experiment: DIP-220179X Structure (London, England : 1993) 2011 IMEx Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. Only protein-protein interactions imex curation patino-2011-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. DIP original experiment: DIP-220176X Structure (London, England : 1993) 2011 IMEx Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. Only protein-protein interactions imex curation patino-2011-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. DIP original experiment: DIP-220177X Structure (London, England : 1993) 2011 IMEx Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. Only protein-protein interactions imex curation patino-2011-4 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. DIP original experiment: DIP-220178X Structure (London, England : 1993) 2011 IMEx Structure analysis of the IL-5 ligand-receptor complex reveals a wrench-like architecture for IL-5Rα. Patino E., Kotzsch A., Saremba S., Nickel J., Schmitz W., Sebald W., Mueller TD. Only protein-protein interactions imex curation q01344-2 Interleukin-5 receptor subunit alpha IL5RA IL5R Soluble-S1 CDw125 protein protein human Homo sapiens Human MIIVAHVLLILLGATEILQADLLPDEKISLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNVNLEYQVKINAPKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHAPPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVNGSSKHSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQPIYVGFSR il5_human Interleukin-5 IL5 T-cell replacing factor Eosinophil differentiation factor B-cell differentiation factor I protein protein human Homo sapiens Human MRMLLHLSLLALGAAYVYAIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES il5-il5ra-3 3233751 N/A 3233755 unspecified role unspecified role prey prey Extracellular domain Region 21-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3233756 unspecified role unspecified role bait bait P05113:p.Arg110Ala R110A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false il5-il5ra-4 3233752 N/A 3233755 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 21-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3233756 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false il5-il5ra-5 3233753 N/A 3233755 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 21-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3233756 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false il5-il5ra-6 3233754 N/A 3233755 unspecified role unspecified role prey prey Extracellular domain Region 21-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Glu78Pro E78P mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Asp75Glu D75E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Arg80Ala R80A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Ser84Ala S84A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Ile89Ala I89A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Trp178Ala W178A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Ile203Ala I203A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Arg208Lys R208K mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Asp228Ala D228A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Met315Ala M315A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Leu43Ala L43A mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q01344-2:p.Tyr77Ala Y77A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3233756 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false