DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ rai-2011-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. DIP original experiment: DIP-220461X Nature structural & molecular biology 2011 IMEx The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection. Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. Only protein-protein interactions imex curation rai-2011-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. DIP original experiment: DIP-220459X Nature structural & molecular biology 2011 IMEx The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection. Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. Only protein-protein interactions imex curation rai-2011-3 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. DIP original experiment: DIP-220465X Nature structural & molecular biology 2011 IMEx The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection. Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. Only protein-protein interactions imex curation rai-2011-4 human Homo sapiens Human pull down pull down predetermined predetermined participant predetermined Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. DIP original experiment: DIP-220463X Nature structural & molecular biology 2011 IMEx The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection. Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. Only protein-protein interactions imex curation rai-2011-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. DIP original experiment: DIP-220462X Nature structural & molecular biology 2011 IMEx The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection. Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. Only protein-protein interactions imex curation rai-2011-6 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. DIP original experiment: DIP-220460X Nature structural & molecular biology 2011 IMEx The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection. Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. Only protein-protein interactions imex curation rai-2011-7 human Homo sapiens Human pull down pull down predetermined predetermined participant predetermined Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. DIP original experiment: DIP-220464X Nature structural & molecular biology 2011 IMEx The E3 ubiquitin ligase Rnf8 stabilizes Tpp1 to promote telomere end protection. Rai R., Li JM., Zheng H., Lok GT., Deng Y., Huen MS., Chen J., Jin J., Chang S. Only protein-protein interactions imex curation pote1_mouse Protection of telomeres protein 1 Pot1 Pot1a POT1-like telomere end-binding protein protein protein mouse Mus musculus Mouse MSLVSTAPYTYTPLNLLKEGTIANVYGVVKFFKPPYVSKGTDYCSVVTIVDQTNVKLTCMLFSGNYEALPIIYKVGDIVRFHRLKIQVYKNELQGINCSGFASLTFEGTVGMPVTARTSSKVFSFTPQDQKMVEALRVWASKHISASSTLVQLCDAQPMQYYDLTCQLLGKAQVDSTAFLLKVWDGTQTVLPSWRVSTQDLTFEGDLSHIERLQSLVVDILVYDNHVQVARSIEVGCFLRLYSLHTKLQPGNSETSSSESLRLEFHLHGGTSYGRGIRVLPDTSPCVDQLKKALEGANLPVTETSTGICQSENGDSSALSNSGSGAVSPYEEERCQQVSATILTNHQHLEKTPLCAILTQKAPQQYRVRAKLRSYLPRRLSQSVKLLCPKCHSVQEVPHGDSLDKILQDAATEAPDIKLKATSLYYSKVWTTEDQGGRQVAVHFVKNNGILPASSECLILIEGGRLCEVSKLSSKFHSVMPVRSGPESLELLTLSAPFLIQGKVHHYGCKQCSSLKPIQNLNSRFHKGPWTPSSVAEALGVVPLQYVFVMVFTLDDGTGVLEAYLKDSEHFFKIPASEVLTDDDLQRSLETIMDMICPPGIKVDAYPWLECLLKSYNVTIGTERRICYQIFDTTVAENVV mint Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity acd_human Adrenocortical dysplasia protein homolog POT1 and TIN2-interacting protein ACD PIP1 PTOP TINT1 TPP1 protein protein human Homo sapiens Human MAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTLIASPPSSPSSSGTPALPGHMSSEESGTSISLLPALSLAAPDPGQRSSSQPSPAICSAPATLTPRSPHASRTPSSPLQSCTPSLSPRSHVPSPHQALVTRPQKPSLEFKEFVGLPCKNRPPFPRTGATRGAQEPCSVWEPPKRHRDGSAFQYEYEPPCTSLCARVQAVRLPPQLMAWALHFLMDAQPGSEPTPM rnf8_mouse E3 ubiquitin-protein ligase RNF8 Rnf8 ActA-interacting protein 37 LaXp180 RING finger protein 8 RING-type E3 ubiquitin transferase RNF8 protein protein mouse Mus musculus Mouse MGEPDPLVSGQLAARRSWCLRRLGMDCEWLQLEAGTEVTIGRGLSVTYQLISKVCPLMISRSHCVLKQNPEGQWTIMDNKSLNGVWLNRERLAPLQGYCIRKGDHIQLGVPLESRETAEYEYEVIEEDWESLAPCLAPKNDQRMEKHKGSRTKRKFSSPGLENLPAEGSSDLRCPLANVASKPIEPEKLHGKGDASSQSLGCLCPGLTSLKASERAAGPHACSALPKVLELSCPKKQKACRPSASQNSLELFKVTMSRMLKLKTQMQEKQIAVLNVKRQTRKGSSKKIVRMEKELRNLQSQLYAEQAQQQARVEQLEKTFQEEAHYLQGLEKEQGECDLKQQLVQALQEHQALMEELNCSKKDFEKIIQAKNKELEQTKEEKDKVQAQKEEVLSHMNDLLENELQCIICSEYFIEAVTLNCAHSFCSFCINEWMKRKVECPICRKDIESRTNSLVLDNCISKMVDNLSSDVKERRSVLIRERRAKRLS ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG acd-pot1-9 3232966 N/A 3232973 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3232974 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false acd-rnf8-1 3232967 N/A 3232975 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3232974 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false acd-mub-1 3232968 N/A 3232976 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3232974 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse physical association physical association false false false hub-acd-1 3232969 N/A 3232977 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3232974 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false pot1-acd-6 3232970 N/A 3232973 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3232974 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false rnf8-acd-1 3232971 N/A 3232975 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3232974 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false hub-acd-2 3232972 N/A 3232977 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3232974 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false