DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bolanos_garcia-2011-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252389X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252378X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252386X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-4 in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252382X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-5 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252379X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252383X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-7 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252380X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-8 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252384X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-9 in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252387X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-10 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252390X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-11 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252381X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-12 in vitro In vitro cd circular dichroism CD CD cd predetermined predetermined participant predetermined Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252385X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation bolanos_garcia-2011-13 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. DIP original experiment: DIP-252388X Structure (London, England : 1993) 2011 IMEx Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site. Bolanos-Garcia VM., Lischetti T., Matak-Vinković D., Cota E., Simpson PJ., Chirgadze DY., Spring DR., Robinson CV., Nilsson J., Blundell TL. Only protein-protein interactions imex curation knl1_human Kinetochore scaffold 1 KNL1 ALL1-fused gene from chromosome 15q14 protein Bub-linking kinetochore protein CASC5 Cancer susceptibility candidate gene 5 protein Cancer/testis antigen 29 KIAA1570 Kinetochore-null protein 1 Protein CASC5 Protein D40/AF15q14 protein protein human Homo sapiens Human MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEGCSAMVPSQLQLLPPGFKRFSCLSLPETETGENLLLIQNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH bub1b_human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta MAD3/BUB1-related protein kinase Mitotic checkpoint kinase MAD3L SSK1 BUB1B BUBR1 MAD3L Protein SSK1 protein protein human Homo sapiens Human MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFESSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFDENADEASTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVPAVLPSFTPYVEETARQPVMTPCKIEPSINHILSTRKPGKEEGDPLQRVQSHQQASEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSSSVCQVNCCARETSLAENIWQEQPHSKGPSVPFSIFDEFLLSEKKNKSPPADPPRVLAQRRPLAVLKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFVSTPFHEIMSLKDLPSDPERLLPEEDLDVKTSEDQQTACGTIYSQTLSIKKLSPIIEDSREATHSSGFSGSSASVASTSSIKCLQIPEKLELTNETSENPTQSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ bub3_human Mitotic checkpoint protein BUB3 BUB3 protein protein human Homo sapiens Human MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT cdc20_human Cell division cycle protein 20 homolog CDC20 p55CDC protein protein human Homo sapiens Human MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSASSGDILQLLQMEQPGEYISSVAWIKEGNYLAVGTSSAEVQLWDVQQQKRLRNMTSHSARVGSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNLVNVWPSAPGEGGWVPLQTFTQHQGAVKAVAWCPWQSNVLATGGGTSDRHIRIWNVCSGACLSAVDAHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTSRVLSLTMSPDGATVASAAADETLRLWRCFELDPARRREREKASAAKSSLIHQGIR md2l1_human Mitotic spindle assembly checkpoint protein MAD2A MAD2L1 MAD2 Mitotic arrest deficient 2-like protein 1 protein protein human Homo sapiens Human MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND q8ng31-2 Kinetochore scaffold 1 KNL1 ALL1-fused gene from chromosome 15q14 protein Bub-linking kinetochore protein CASC5 Cancer susceptibility candidate gene 5 protein Cancer/testis antigen 29 KIAA1570 Kinetochore-null protein 1 Protein CASC5 Protein D40/AF15q14 protein protein human Homo sapiens Human MDGVSSEANEENDNIERPVRRRHSSILKPPRSPLQDLRGGNERVQESNALRNKKNSRRVSFADTIKVFQTESHMKIVRKSEMEETETGENLLLIQNKKLEDNYCEITGMNTLLSAPIHTQMQQKEFSIIEHTRERKHANDQTVIFSDENQMDLTSSHTVMITKGLLDNPISEKSTKIDTTSFLANLKLHTEDSRMKKEVNFSVDQNTSSENKIDFNDFIKRLKTGKCSAFPDVPDKENFEIPIYSKEPNSASSTHQMHVSLKEDENNSNITRLFREKDDGMNFTQCHTANIQTLIPTSSETNSRESKGNDITIYGNDFMDLTFNHTLQILPATGNFSEIENQTQNAMDVTTGYGTKASGNKTVFKSKQNTAFQDLSINSADKIHITRSHIMGAETHIVSQTCNQDARILAMTPESIYSNPSIQGCKTVFYSSCNDAMEMTKCLSNMREEKNLLKHDSNYAKMYCNPDAMSSLTEKTIYSGEENMDITKSHTVAIDNQIFKQDQSNVQIAAAPTPEKEMMLQNLMTTSEDGKMNVNCNSVPHVSKERIQQSLSNPLSISLTDRKTELLSGENMDLTESHTSNLGSQVPLAAYNLAPESTSESHSQSKSSSDECEEITKSRNEPFQRSDIIAKNSLTDTWNKDKDWVLKILPYLDKDSPQSADCNQEIATSHNIVYCGGVLDKQITNRNTVSWEQSLFSTTKPLFSSGQFSMKNHDTAISSHTVKSVLGQNSKLAEPLRKSLSNPTPDYCHDKMIICSEEEQNMDLTKSHTVVIGFGPSELQELGKTNLEHTTGQLTTMNRQIAVKVEKCGKSPIEKSGVLKSNCIMDVLEDESVQKPKFPKEKQNVKIWGRKSVGGPKIDKTIVFSEDDKNDMDITKSYTIEINHRPLLEKRDCHLVPLAGTSETILYTCRQDDMEITRSHTTALECKTVSPDEITTRPMDKTVVFVDNHVELEMTESHTVFIDYQEKERTDRPNFELSQRKSLGTPTVICTPTEESVFFPGNGESDRLVANDSQLTPLEEWSNNRGPVEVADNMELSKSATCKNIKDVQSPGFLNEPLSSKSQRRKSLKLKNDKTIVFSENHKNDMDITQSCMVEIDNESALEDKEDFHLAGASKTILYSCGQDDMEITRSHTTALECKTLLPNEIAIRPMDKTVLFTDNYSDLEVTDSHTVFIDCQATEKILEENPKFGIGKGKNLGVSFPKDNSCVQEIAEKQALAVGNKIVLHTEQKQQLFAATNRTTNEIIKFHSAAMDEKVIGKVVDQACTLEKAQVESCQLNNRDRRNVDFTSSHATAVCGSSDNYSCLPNVISCTDNLEGSAMLLCDKDEEKANYCPVQNDLAYANDFASEYYLESEGQPLSAPCPLLEKEEVIQTSTKGQLDCVITLHKDQDLIKDPRNLLANQTLVYSQDLGEMTKLNSKRVSFKLPKDQMKVYVDDIYVIPQPHFSTDQPPLPKKGQSSINKEEVILSKAGNKSLNIIENSSAPICENKPKILNSEEWFAAACKKELKENIQTTNYNTALDFHSNSDVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQTVHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKSHNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDINHLETQPVSSKDSGIGSVAGKLNLSPSQYINEENLPVYPDEINSSDSINIETEEKALIETYQKEISPYENKMGKTCNSQKRTWVQEEEDIHKEKKIRKNEIKFSDTTQDREIFDHHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQMESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNFTVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKLLVDINKNLWEKMRHCSDKELKAFGIYLNKIKSCFTKMTKVFTHQGKVALYGKLVQSAQNEREKLQIKIDEMDKILKKIDNCLTEMETETKNLEDEEKNNPVEEWDSEMRAAEKELEQLKTEEEELQRNLLELEVQKEQTLAQIDFMQKQRNRTEELLDQLSLSEWDVVEWSDDQAVFTFVYDTIQLTITFEESVVGFPFLDKRYRKIVDVNFQSLLDEDQAPPSSLLVHKLIFQYVEEKESWKKTCTTQHQLPKMLEEFSLVVHHCRLLGEEIEYLKRWGPNYNLMNIDINNNELRLLFSSSAAFAKFEITLFLSAYYPSVPLPSTIQNHVGNTSQDDIATILSKVPLENNYLKNVVKQIYQDLFQDCHFYH bub1b-bub3-2 3231214 N/A 3231227 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3231228 unspecified role unspecified role bait bait Venus-tag Venus-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3231229 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3231230 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3231231 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false knl1-bub1b-1 3231215 N/A 3231228 unspecified role unspecified role prey prey Region 57-220 Region 57-220 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3231232 unspecified role unspecified role bait bait Region 227-250 Region 227-250 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false bub1b-knl1-1 3231216 N/A 3231228 unspecified role unspecified role unspecified role unspecified role Region 57-220 Region 57-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O60566:p.[Tyr141Ala;Leu142Ala] Y141A/L142A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O60566:p.[Leu128Ala;Leu131Ala] L128A/L131A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231232 unspecified role unspecified role unspecified role unspecified role Region 212-222 Region 212-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false bub1b-knl1-2 3231217 N/A 3231228 unspecified role unspecified role unspecified role unspecified role Region 57-220 Region 57-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231232 unspecified role unspecified role unspecified role unspecified role Region 212-222 Region 212-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q8NG31-2:p.[Ile213Ala;Phe215Ala;Phe218Ala] I213A/F215A/F218A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8NG31-2:p.[Phe215Ala;Phe218Ala] F215A/F218A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8NG31-2:p.[Phe215Ala;Ile219Ala] F215A/I219A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false knl1-bub1b-2 3231218 N/A 3231228 unspecified role unspecified role prey prey Region 57-220 Region 57-220 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O60566:p.[Leu128Ala;Leu131Ala] L128A/L131A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O60566:p.[Tyr141Ala;Leu142Ala] Y141A/L142A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3231232 unspecified role unspecified role bait bait Region 225-250 Region 225-250 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false bub1b-knl1-3 3231219 N/A 3231228 unspecified role unspecified role unspecified role unspecified role Region 57-220 Region 57-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231232 unspecified role unspecified role unspecified role unspecified role Region 208-226 Region 208-226 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false bub3-bub1b-1 3231220 N/A 3231228 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3231229 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false bub1b-knl1-4 3231221 N/A 3231228 unspecified role unspecified role unspecified role unspecified role Region 57-220 Region 57-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231232 unspecified role unspecified role unspecified role unspecified role Region 208-226 Region 208-226 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false bub1b-knl1-5 3231222 N/A 3231228 unspecified role unspecified role unspecified role unspecified role Region 57-220 Region 57-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O60566:p.[Tyr141Ala;Leu142Ala] Y141A/L142A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O60566:p.[Leu128Ala;Leu131Ala] L128A/L131A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231232 unspecified role unspecified role unspecified role unspecified role Region 212-222 Region 212-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false bub1b-knl1-6 3231223 N/A 3231228 unspecified role unspecified role unspecified role unspecified role Region 57-220 Region 57-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231232 unspecified role unspecified role unspecified role unspecified role Region 208-226 Region 208-226 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false bub1b-knl1-7 3231224 N/A 3231228 unspecified role unspecified role unspecified role unspecified role Region 57-220 Region 57-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231232 unspecified role unspecified role unspecified role unspecified role Region 212-222 Region 212-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q8NG31-2:p.[Ile213Ala;Phe215Ala;Phe218Ala] I213A/F215A/F218A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8NG31-2:p.[Phe215Ala;Phe218Ala] F215A/F218A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8NG31-2:p.[Phe215Ala;Ile219Ala] F215A/I219A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false bub1b-knl1-8 3231225 N/A 3231228 unspecified role unspecified role unspecified role unspecified role Region 57-220 Region 57-220 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3231232 unspecified role unspecified role unspecified role unspecified role Region 208-226 Region 208-226 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false bub1b-cdc20-4 3231226 N/A 3231228 unspecified role unspecified role bait bait O60566:p.[Tyr141Ala;Leu142Ala] Y141A/L142A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O60566:p.[Leu128Ala;Leu131Ala] L128A/L131A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Venus-tag Venus-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3231230 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3231231 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false