DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kmmel-2011-1 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. DIP original experiment: DIP-218667X Nature structural & molecular biology 2011 IMEx Complexin cross-links prefusion SNAREs into a zigzag array. Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. Only protein-protein interactions imex curation kmmel-2011-2 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. DIP original experiment: DIP-218668X Nature structural & molecular biology 2011 IMEx Complexin cross-links prefusion SNAREs into a zigzag array. Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. Only protein-protein interactions imex curation kmmel-2011-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. DIP original experiment: DIP-218672X Nature structural & molecular biology 2011 IMEx Complexin cross-links prefusion SNAREs into a zigzag array. Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. Only protein-protein interactions imex curation kmmel-2011-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. DIP original experiment: DIP-218671X Nature structural & molecular biology 2011 IMEx Complexin cross-links prefusion SNAREs into a zigzag array. Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. Only protein-protein interactions imex curation kmmel-2011-5 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. DIP original experiment: DIP-218670X Nature structural & molecular biology 2011 IMEx Complexin cross-links prefusion SNAREs into a zigzag array. Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. Only protein-protein interactions imex curation kmmel-2011-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. DIP original experiment: DIP-218669X Nature structural & molecular biology 2011 IMEx Complexin cross-links prefusion SNAREs into a zigzag array. Kümmel D., Krishnakumar SS., Radoff DT., Li F., Giraudo CG., Pincet F., Rothman JE., Reinisch KM. Only protein-protein interactions imex curation p60880-2 Synaptosomal-associated protein 25 SNAP25 SNAP Super protein Synaptosomal-associated 25 kDa protein SNAP-25a protein protein human Homo sapiens Human MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG stx1a_rat Syntaxin-1A Synaptotagmin-associated 35 kDa protein Neuron-specific antigen HPC-1 Stx1a Sap protein protein rat Rattus norvegicus (Rat) Rat MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKKIMIIICCVILGIIIASTIGGIFG vamp2_human Vesicle-associated membrane protein 2 Synaptobrevin-2 VAMP2 SYB2 protein protein human Homo sapiens Human MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST cplx1_human Complexin-1 Complexin I Synaphin-2 CPLX1 protein protein human Homo sapiens Human MEFVMKQALGGATKDMGKMLGGDEEKDPDAAKKEEERQEALRQAEEERKAKYAKMEAEREAVRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTVIKYLPGPLQDMLKK cplx1-snap25-4 3228778 N/A 3228784 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 141-203 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228785 unspecified role unspecified role unspecified role unspecified role Region 191-253 Region 191-253 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228786 unspecified role unspecified role unspecified role unspecified role Region 29-60 Region 29-60 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228787 unspecified role unspecified role unspecified role unspecified role Region 26-83 Region 26-83 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228784 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 7-82 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false snap25-cplx1-2 3228779 N/A 3228784 unspecified role unspecified role fluorescence donor fluorescence donor C-terminal domain Region 141-203 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stilbene-label Stilbene-label fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228787 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-134 Region 1-134 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Bimane-label Bimane-label bimane bimane label bimane certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cplx1-snap25-5 3228780 N/A 3228784 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 141-203 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228785 unspecified role unspecified role unspecified role unspecified role Region 191-253 Region 191-253 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228786 unspecified role unspecified role unspecified role unspecified role Region 29-60 Region 29-60 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228787 unspecified role unspecified role unspecified role unspecified role Region 26-83 Region 26-83 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228784 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 7-82 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false snap25-snap25-5 3228781 N/A 3228784 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 141-203 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228785 unspecified role unspecified role unspecified role unspecified role Region 191-253 Region 191-253 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228786 unspecified role unspecified role unspecified role unspecified role Region 29-60 Region 29-60 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228784 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 7-82 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false snap25-cplx1-3 3228782 N/A 3228784 unspecified role unspecified role fluorescence donor fluorescence donor C-terminal domain Region 141-203 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stilbene-label Stilbene-label fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228787 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-134 Region 1-134 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Bimane-label Bimane-label bimane bimane label bimane certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false snap25-snap25-6 3228783 N/A 3228784 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 141-203 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228785 unspecified role unspecified role unspecified role unspecified role Region 191-253 Region 191-253 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228786 unspecified role unspecified role unspecified role unspecified role Region 29-60 Region 29-60 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3228784 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 7-82 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false