DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ guo-2011-3 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. DIP original experiment: DIP-218600X Nature structural & molecular biology 2011 IMEx An ALS-associated mutation affecting TDP-43 enhances protein aggregation, fibril formation and neurotoxicity. Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. Only protein-protein interactions imex curation guo-2011-4 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. DIP original experiment: DIP-218603X Nature structural & molecular biology 2011 IMEx An ALS-associated mutation affecting TDP-43 enhances protein aggregation, fibril formation and neurotoxicity. Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. Only protein-protein interactions imex curation guo-2011-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. DIP original experiment: DIP-218604X Nature structural & molecular biology 2011 IMEx An ALS-associated mutation affecting TDP-43 enhances protein aggregation, fibril formation and neurotoxicity. Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. Only protein-protein interactions imex curation guo-2011-6 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. DIP original experiment: DIP-218601X Nature structural & molecular biology 2011 IMEx An ALS-associated mutation affecting TDP-43 enhances protein aggregation, fibril formation and neurotoxicity. Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. Only protein-protein interactions imex curation guo-2011-7 in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. DIP original experiment: DIP-218602X Nature structural & molecular biology 2011 IMEx An ALS-associated mutation affecting TDP-43 enhances protein aggregation, fibril formation and neurotoxicity. Guo W., Chen Y., Zhou X., Kar A., Ray P., Chen X., Rao EJ., Yang M., Ye H., Zhu L., Liu J., Xu M., Yang Y., Wang C., Zhang D., Bigio EH., Mesulam M., Shen Y., Xu Q., Fushimi K., Wu JY. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG tadbp_human TAR DNA-binding protein 43 TARDBP TDP43 protein protein human Homo sapiens Human MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM tardbp-ub-1 3227271 N/A 3227276 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3227277 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false tardbp-5 3227272 N/A 3227277 unspecified role unspecified role unspecified role unspecified role Region 286-331 Region 286-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false tardbp-6 3227273 N/A 3227277 unspecified role unspecified role unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false tardbp-7 3227274 N/A 3227277 unspecified role unspecified role unspecified role unspecified role Region 286-331 Region 286-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false tardbp-8 3227275 N/A 3227277 unspecified role unspecified role unspecified role unspecified role Region 286-331 Region 286-331 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false