DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2011-6 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. DIP original experiment: DIP-219159X Nature 2011 IMEx Structure and mechanism of the hexameric MecA-ClpC molecular machine. Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. Only protein-protein interactions imex curation wang-2011-7 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. DIP original experiment: DIP-219161X Nature 2011 IMEx Structure and mechanism of the hexameric MecA-ClpC molecular machine. Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. Only protein-protein interactions imex curation wang-2011-8 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. DIP original experiment: DIP-219160X Nature 2011 IMEx Structure and mechanism of the hexameric MecA-ClpC molecular machine. Wang F., Mei Z., Qi Y., Yan C., Hu Q., Wang J., Shi Y. Only protein-protein interactions imex curation clpc_bacsu Negative regulator of genetic competence ClpC/MecB clpC mecB BSU00860 protein protein bacsu Bacillus subtilis MMFGRFTERAQKVLALAQEEALRLGHNNIGTEHILLGLVREGEGIAAKALQALGLGSEKIQKEVESLIGRGQEMSQTIHYTPRAKKVIELSMDEARKLGHSYVGTEHILLGLIREGEGVAARVLNNLGVSLNKARQQVLQLLGSNETGSSAAGTNSNANTPTLDSLARDLTAIAKEDSLDPVIGRSKEIQRVIEVLSRRTKNNPVLIGEPGVGKTAIAEGLAQQIINNEVPEILRDKRVMTLDMGTVVAGTKYRGEFEDRLKKVMDEIRQAGNIILFIDELHTLIGAGGAEGAIDASNILKPSLARGELQCIGATTLDEYRKYIEKDAALERRFQPIQVDQPSVDESIQILQGLRDRYEAHHRVSITDDAIEAAVKLSDRYISDRFLPDKAIDLIDEAGSKVRLRSFTTPPNLKELEQKLDEVRKEKDAAVQSQEFEKAASLRDTEQRLREQVEDTKKSWKEKQGQENSEVTVDDIAMVVSSWTGVPVSKIAQTETDKLLNMENILHSRVIGQDEAVVAVAKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESIFGDEESMIRIDMSEYMEKHSTSRLVGSPPGYVGYDEGGQLTEKVRRKPYSVVLLDEIEKAHPDVFNILLQVLEDGRLTDSKGRTVDFRNTILIMTSNVGASELKRNKYVGFNVQDETQNHKDMKDKVMGELKRAFRPEFINRIDEIIVFHSLEKKHLTEIVSLMSDQLTKRLKEQDLSIELTDAAKAKVAEEGVDLEYGARPLRRAIQKHVEDRLSEELLRGNIHKGQHIVLDVEDGEFVVKTTAKTN 9592231886C2E870 mint Competence gene repressor; required for cell growth at high temperature. Negative regulator of comK expression. May interact with mecA to negatively regulate comK meca1_bacsu Adapter protein MecA 1 mecA BSU11520 protein protein bacsu Bacillus subtilis MEIERINEHTVKFYMSYGDIEDRGFDREEIWYNRERSEELFWEVMDEVHEEEEFAVEGPLWIQVQALDKGLEIIVTKAQLSKDGQKLELPIPEDKKQEPASEDLDALLDDFQKEEQAVNQEEKEQKLQFVLRFGDFEDVISLSKLNVNGSKTTLYSFENRYYLYVDFCNMTDEEVENQLSILLEYATESSISIHRLEEYGKLIISEHALETIKKHFAS F6E377D8EA241B7D clpc-meca-4 3223018 N/A 3223021 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false D1-M domain Region 150-485 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3223022 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 121-218 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P37958:p.Glu184Arg E184R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clpc-meca-5 3223019 N/A 3223021 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3223022 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 108-218 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false clpc-meca-6 3223020 N/A 3223021 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3223022 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 121-218 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false