DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lim-2011-5 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. DIP original experiment: DIP-219167X Nature 2011 IMEx The novel gene twenty-four defines a critical translational step in the Drosophila clock. Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. Only protein-protein interactions imex curation lim-2011-6 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. DIP original experiment: DIP-219169X Nature 2011 IMEx The novel gene twenty-four defines a critical translational step in the Drosophila clock. Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. Only protein-protein interactions imex curation lim-2011-7 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. DIP original experiment: DIP-219170X Nature 2011 IMEx The novel gene twenty-four defines a critical translational step in the Drosophila clock. Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. Only protein-protein interactions imex curation lim-2011-8 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. DIP original experiment: DIP-219168X Nature 2011 IMEx The novel gene twenty-four defines a critical translational step in the Drosophila clock. Lim C., Lee J., Choi C., Kilman VL., Kim J., Park SM., Jang SK., Allada R., Choe J. Only protein-protein interactions imex curation pabp_drome Polyadenylate-binding protein pAbp CG5119 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASLYVGDLPQDVNESGLFDKFSSAGPVLSIRVCRDVITRRSLGYAYVNFQQPADAERALDTMNFDLVRNKPIRIMWSQRDPSLRRSGVGNVFIKNLDRAIDNKAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREKELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGKDMGEGKSLYVARAQKKAERQQELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVALAQRKEERKAHLASQYMRHMTGMRMQQLGQIYQPNAASGFFVPTLPSNQRFFGSQVATQMRNTPRWVPQVRPPAAIQGVQAGAAAAGGFQGTAGAVPTQFRSAAAGARGAQPQVQGTHAAAAAANNMRNTGARAITGQQTAAPNMQIPGAQIAGGAQQRTSNYKYTSNMRNPPVPQLHQTQPIPQQLQGKNSEKLIASLLANAKPQEQKQILGERLYPMIEHMHANLAGKITGMLLEIENSELLHMIEDQEALKAKVEEAVAVLQVHRVTEPAN q9w4m7_drome tyf CG485 Dmel\CG4857 EG:EG0007.12 EG:EG0007.4 TYF Dmel_CG4857 CG4857 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNNEQTPSSSDTPADRVASGGAAAKLPTIIPDTPIVSIAAGTMSESTTTTTVTKTTPTPFPQEQLMTMMSDHVAKADAQLDEACRNQGAKDEDSQGAAPVAANHVATSGSSSRGNDPKKSSSPSNSHSSGNHAATADAAKGSAAEAAAGDTSFSQVFAMLKDSPSAELSRPNRRVLQYGATAKGKTQDDQNVGKRQKKKKTKYDTWEDSDFNDLDDASLKKLLEEAYWYRNPGDRKNKSERFLQMLKKAEYDEEISYRAIKSCLTINPSALTSSAATGSSGGSSSSAGHNSSYNSTNHRSTDPGQRHKQGGSLQDLVEAAHRSELATTSAAAAAAATAAATQRFNCDYQLSGRSNRRQQQQQQQHNQNATSSASASKKIKNLNVSGRQREGGSLPSSVNFEPASSAMEAKQNKQQAQPSTEAAGGSFSGSVGNKHHHRSSTGSCSSLITQSRDTEANNQFDLDMDDATGVGASAVVGASGDQDDNMLEQLDPQNPSMKFLIDALNDTPLEARYTGAGKKFLIDDRLHFSVLELSARRKLQQQAQYLAALTGQFGLGLEEEKRKVEAGYVSLNDNYTACSSVYGGGAGSPAAVEGSKPSTSSSAVSGGSNCGASSDALSSDLRPAKIGRMVPAAAAVAAAPMGGKTTTRQLDENGNALGDGFGGSAASGSSGASGASAAASSSNGNGNANQFTALITPTVGSSVPTSTVAHRQNSVSTLLSTGTNTTTTAMAAAAVAASYSQLQQAPVGGAAGTVSGIGIGMQQPTKQKTKKKSQQERNTTTVDVESVAGYRGNDPVEQLVKYIENDVNGGGNSAAGQRKKERKKQNKLKKSNSLEELRTCSKMEVDDLKRQSATTEMMRHKKGTNNASSGGSSSTASGSSNSGKHNSASVADINKNCNKEQQQQSTPTVQVRNSNNPGTQQRKGERRSWGTEELQYLGDHQEISAAWSELETVGQKELPLALPALARMSELDALNTVLSETAEFHVVTKKKKPKKQRAVTMDDAAVAAAAVTGGNLQRMQQITKSASSNIMSQRMHYYTPYNSNNGGGSVNYKQQQQQSQQYQEHYQAPQGQHHHHHHHHHHHHHHHSGSAVSNQVDSSRRKSTSSMPPSEKSDSSDLDSVHSLPIQTGKKKSLGGGNNKQRGAPSASQRQAKQNNNAAAPISYADIARNKQEALNNATASDTELELGDKMQIKSGGKSKSRPDFPELPGAVTAVAASGGAANQATVVSSQNTPPSSSSSISYSQSLNATPPSSSSDAESTNSPELLAQVQIPAYSMITPTLTASMISSVSSSVASTSSTSCSPPALQKSKSVEHDTSYSCNSSNLDQQYPALEKTVKRHSTNNVSVAVAASTSASASSSLYNFAAAAKQQLTENTHSAVSPPAAVTTTSTAVAASAPAPTTVTVPAAVTVPCYSSSSTHGSTQTSTSTASSAIAALPSGKAKAKPKELSPSSSCSKKPTKPSQDVPSISLSISTTPRATTSTSTTTTQKTTAATQTEGAKKLIGGIHKLPSSSCIAKGAGAVLEATAGRRAVIILNDDREAGRSNNEFIFGDFNEDELKLFDDNLDDEEEGQHKQSSNKKQQTEGESDVTQDDTEDVDQDLDKELECLGRRPEKKQERQQDISASSDQHLNDSGAASDAVNSSASLDMLSISAEAAQSSPNAGATATSSSVASPINSSTPSGASSASASTSTSSSSVSISSSSGGNGAACLASVSGMLGGVANQSGDSGIYAAANTSIKEHVNTFLSKASSSEETLPSPNSISMQQLETCNDIEAAIIAAARAAAAARSTSCSRSNSQEQDALQPQVKTKATPNPEAKMVLPVFVTYNNNNNDDDDDEEDDESLQELSFMADLKADAATEDIAMQPPPPSCPAMMTNTELIVDYIAKTWNAIANSKYVTYYNEQEQEQET if4e_drome Eukaryotic translation initiation factor 4E1 eIF4E1 Eif4e eIF-4E eIF-4F 25 kDa subunit mRNA cap-binding protein CG4035 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSDFHRMKNFANPKSMFKTSAPSTEQGRPEPPTSAAAPAEAKDVKPKEDPQETGEPAGNTATTTAPAGDDAVRTEHLYKHPLMNVWTLWYLENDRSKSWEDMQNEITSFDTVEDFWSLYNHIKPPSEIKLGSDYSLFKKNIRPMWEDAANKQGGRWVITLNKSSKTDLDNLWLDVLLCLIGEAFDHSDQICGAVINIRGKSNKISIWTADGNNEEAALEIGHKLRDALRLGRNNSLQYQLHKDTMVKQGSNVKSIYTL tyf-pabp-1 3222727 N/A 3222731 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3222732 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false eif_4e-pabp-1 3222728 N/A 3222733 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3222731 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false pabp-tyf-1 3222729 N/A 3222731 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3222732 unspecified role unspecified role prey prey N-terminal domain Region 1-1167 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false eif_4e-pabp-2 3222730 N/A 3222733 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3222731 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3222732 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly association association false false false