DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ morgan-2011-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218498X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions imex curation morgan-2011-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218496X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions imex curation morgan-2011-3 in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218500X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions imex curation morgan-2011-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218497X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions imex curation morgan-2011-5 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. DIP original experiment: DIP-218499X Nature structural & molecular biology 2011 IMEx Structural basis for engagement by complement factor H of C3b on a self surface. Morgan HP., Schmidt CQ., Guariento M., Blaum BS., Gillespie D., Herbert AP., Kavanagh D., Mertens HD., Svergun DI., Johansson CM., Uhrín D., Barlow PN., Hannan JP. Only protein-protein interactions imex curation cfah_human Complement factor H CFH HF HF1 HF2 H factor 1 protein protein human Homo sapiens Human MRLLAKIICLMLWAICVAEDCNELPPRRNTEILTGSWSDQTYPEGTQAIYKCRPGYRSLGNVIMVCRKGEWVALNPLRKCQKRPCGHPGDTPFGTFTLTGGNVFEYGVKAVYTCNEGYQLLGEINYRECDTDGWTNDIPICEVVKCLPVTAPENGKIVSSAMEPDREYHFGQAVRFVCNSGYKIEGDEEMHCSDDGFWSKEKPKCVEISCKSPDVINGSPISQKIIYKENERFQYKCNMGYEYSERGDAVCTESGWRPLPSCEEKSCDNPYIPNGDYSPLRIKHRTGDEITYQCRNGFYPATRGNTAKCTSTGWIPAPRCTLKPCDYPDIKHGGLYHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDHIHCTQDGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGKSIDVACHPGYALPKAQTTVTCMENGWSPTPRCIRVKTCSKSSIDIENGFISESQYTYALKEKAKYQCKLGYVTADGETSGSITCGKDGWSAQPTCIKSCDIPVFMNARTKNDFTWFKLNDTLDYECHDGYESNTGSTTGSIVCGYNGWSDLPICYERECELPKIDVHLVPDRKKDQYKVGEVLKFSCKPGFTIVGPNSVQCYHFGLSPDLPICKEQVQSCGPPPELLNGNVKEKTKEEYGHSEVVEYYCNPRFLMKGPNKIQCVDGEWTTLPVCIVEESTCGDIPELEHGWAQLSSPPYYYGDSVEFNCSESFTMIGHRSITCIHGVWTQLPQCVAIDKLKKCKSSNLIILEEHLKNKKEFDHNSNIRYRCRGKEGWIHTVCINGRWDPEVNCSMAQIQLCPPPPQIPNSHNMTTTLNYRDGEKVSVLCQENYLIQEGEEITCKDGRWQSIPLCVEKIPCSQPPQIEHGTINSSRSSQESYAHGTKLSYTCEGGFRISEENETTCYMGKWSSPPQCEGLPCKSPPEISHGVVAHMSDSYQYGEEVTYKCFEGFGIDGPAIAKCLGEKWSHPPSCIKTDCLSLPSFENAIPMGEKKDVYKAGEQVTYTCATYYKMDGASNVTCINSRWTGRPTCRDTSCVNPPTVQNAYIVSRQMSKYPSGERVRYQCRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYSRTGESVEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKR co3_human Complement C3 C3 CPAMD1 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 protein protein human Homo sapiens Human MGPTSGPSLLLLLLTHLPLALGSPMYSIITPNILRLESEETMVLEAHDAQGDVPVTVTVHDFPGKKLVLSSEKTVLTPATNHMGNVTFTIPANREFKSEKGRNKFVTVQATFGTQVVEKVVLVSLQSGYLFIQTDKTIYTPGSTVLYRIFTVNHKLLPVGRTVMVNIENPEGIPVKQDSLSSQNQLGVLPLSWDIPELVNMGQWKIRAYYENSPQQVFSTEFEVKEYVLPSFEVIVEPTEKFYYIYNEKGLEVTITARFLYGKKVEGTAFVIFGIQDGEQRISLPESLKRIPIEDGSGEVVLSRKVLLDGVQNPRAEDLVGKSLYVSATVILHSGSDMVQAERSGIPIVTSPYQIHFTKTPKYFKPGMPFDLMVFVTNPDGSPAYRVPVAVQGEDTVQSLTQGDGVAKLSINTHPSQKPLSITVRTKKQELSEAEQATRTMQALPYSTVGNSNNYLHLSVLRTELRPGETLNVNFLLRMDRAHEAKIRYYTYLIMNKGRLLKAGRQVREPGQDLVVLPLSITTDFIPSFRLVAYYTLIGASGQREVVADSVWVDVKDSCVGSLVVKSGQSEDRQPVPGQQMTLKIEGDHGARVVLVAVDKGVFVLNKKNKLTQSKIWDVVEKADIGCTPGSGKDYAGVFSDAGLTFTSSSGQQTAQRAELQCPQPAARRRRSVQLTEKRMDKVGKYPKELRKCCEDGMRENPMRFSCQRRTRFISLGEACKKVFLDCCNYITELRRQHARASHLGLARSNLDEDIIAEENIVSRSEFPESWLWNVEDLKEPPKNGISTKLMNIFLKDSITTWEILAVSMSDKKGICVADPFEVTVMQDFFIDLRLPYSVVRNEQVEIRAVLYNYRQNQELKVRVELLHNPAFCSLATTKRRHQQTVTIPPKSSLSVPYVIVPLKTGLQEVEVKAAVYHHFISDGVRKSLKVVPEGIRMNKTVAVRTLDPERLGREGVQKEDIPPADLSDQVPDTESETRILLQGTPVAQMTEDAVDAERLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDETEQWEKFGLEKRQGALELIKKGYTQQLAFRQPSSAFAAFVKRAPSTWLTAYVVKVFSLAVNLIAIDSQVLCGAVKWLILEKQKPDGVFQEDAPVIHQEMIGGLRNNNEKDMALTAFVLISLQEAKDICEEQVNSLPGSITKAGDFLEANYMNLQRSYTVAIAGYALAQMGRLKGPLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKITHRIHWESASLLRSEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN c3-cfh-5 3222617 N/A 3222622 unspecified role unspecified role prey prey CCP19-20 domain Region 1107-1231 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P08603:p.Asp1119Gly D1119G mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P08603:p.Arg1182Ser R1182S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P08603:p.Arg1203Ser R1203S mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P08603:p.Arg1215Gln R1215Q mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3222623 unspecified role unspecified role bait bait C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false c3-cfh-6 3222618 N/A 3222622 unspecified role unspecified role unspecified role unspecified role CCP19-20 domain Region 1107-1231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3222623 unspecified role unspecified role unspecified role unspecified role C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cfh-c3-1 3222619 N/A 3222622 unspecified role unspecified role bait bait CCP19-20 domain Region 1107-1231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3222623 unspecified role unspecified role prey prey C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false c3-cfh-7 3222620 N/A 3222622 unspecified role unspecified role unspecified role unspecified role CCP19-20 domain Region 1107-1231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3222623 unspecified role unspecified role unspecified role unspecified role C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false c3-cfh-8 3222621 N/A 3222622 unspecified role unspecified role prey prey CCP19-20 domain Region 1107-1231 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3222623 unspecified role unspecified role bait bait C3d domain Region 996-1306 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P01024:p.[Glu1110Ala;Asp1115Ala] E1110A/D1115A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P01024:p.Glu1110Ala E1110A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P01024:p.Asp1115Ala D1115A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P01024:p.Glu1153Ala E1153A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P01024:p.Ile1157Ala I1157A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false